Autoxidation of (+)-catechin in three different solutions ( control solution;Tween-20-micellar solution; lecithin-liposomal dispersion) was monitored by HPLC. The rate of (+)-catechin oxidation was higher at pH4.5 than at pH3.5. Comparedwith the control, addition of Tween-20 (micellar structure) and lecithin (liposomal structure) significantly decreased the degra-dation of (+)-catechin. In the presence of lecithin (+)-catechin autoxidation was slower than in the presence of Tween-20.

A strain M014, identified as Trichoderma sp., with high β-1,3-glucanase was isolated from the soil. The effects ofthe different nutrients (carbon source and nitrogen source) and cultural conditions (temperature and time) on β-1,3-glucanaseproduction by M014 were studied. The maximal activity of β-1,3-glucanase was reached at 30℃ at 150r/min with 6d incubationin a liquid medium(pH6.0), containing 3% Fuling powder, 0.5% yeast cream and mineral salts. Enzymatic properties of the crudeenzyme filtration from M014 were investigated. The optimal pH and temperature for the enzyme reaction were pH4.5 and60℃, respectively. At the temperature of 40℃ and 50℃ to be preserved for 12h, the activity of β-1,3 glucanase was still stableHowever, over the temperature of 60℃, it descended rapidly.

Edulis and medicinal fungus were sort of traditional healthy foods of China. Not only Chinese people liked them,but also many people in other countries liked them too. With the increasing of its output and export China, we found more andmore countries asked for the detection of genetically modified ingredients in the fungus. This required the study of transgenicmaterial detection techniques in fungus. The first problem to be resolved was the genomic DNA extraction method and the waysto analyze the quality of the extracted DNA. A high quality extracted genomic DNA was the basis of the following exact resultsof transgenic material detection. So this article set out a study on the genomic DNA extraction method appropriate for most ofthe common edulis and medicinal fungus by analyzing the quality of the extracted genomic DNA.Following 15 species of the common edulis and medicinal fungi(16 samples) were studied here, namely: Pleurotucomucopiae、Volvariella volvacea、Pleurotus sajur-caju、Agrocybe cylindraca maire、Ganoderma lucidum、Russulavinosa Lindbe、Pleurotus eryngii、Lentinus edodes、Agaricus bisporus、Dictyophora indusiata、spore of Ganodermalucidum、Tremella fuciformis Berk、Auricularia auricula、Flammulina velutipes、Pleurotus geesteyanus. Singer andAgaricus blazie. Three commonly used DNA extraction methods were selected, including CTAB method, Food DNA ExtractionKit and Qiagen DNA Extraction Kit. Of them the CTAB method refered for plant introduced in <Short Protocols in MolecularBiology> with a few improvings was adopted. The Food DNA Extraction Kit and Qiagen DNA Extraction Kit refered to theirmethod were provided with the kit. All extracted DNAs were assayed by UV spectrophotometer, agarose electrophoresis andRAPD(Random Amplified Polymorphic DNA) respectively. UV spectrophotometer detection results showed the quality of allextracted genomic DNA was poor as expected. Agarose electrophoresis results showed that all three methods could extract theDNA from the following 7 fungi, Pleurotu comucopiae, Russula vinosa Lindbe, Lentinus edodes, Agaricus bisporus, Dictyophoraindusiata, Auricularia auricula and Agaricus blazie, while CTAB method could extract the DNA of P.geesteyanus.Singer. TheFood DNA Extraction Kit could extract the DNA of V. volvacea and Qiagen DNA Extraction Kit could extract the DNA of T.fuciformis Berk additionally. The RAPD results showed that CTAB method could be used to extract all 15 fungi’ s DNA for PCR,while the other two methods could be used to extract most of the 15 fungi’ s DNA for PCR. According to the above results, wesuggested the choice of the CTAB method for DNA extraction when testing the transgenic ingredients for these edulis and medicinalfungi.All experiment results were confimed by another researcher in our programme.Due to all the values of UV spectrophotometer detection in this study were always abnormal, a comparison with the sameCTAB method on E. coli was made. The results showed that the values of DNA from E. coli were all good. While agaroseelectrophoresis could not be applied to all of the fungi, we had to select RAPD to analyze the DNA for PCR. How to improvethe extraction method to gain high quality DNA from edulis and medicinal fungus if there would be any better method speciallyfor analyzing them would be still needed for further studies.

This test adopted the tap water as the solvent. The conditions for extracting antimicrobial substance from the fruitof Common Macrocarpium have been researched. The optimum extracting condition is A3B2C1. The antimicrobial effect of thesubstance against food spoilage and food-borne pathogens was tested. The results showed that the substance could effectivelyinhibit the growth of bacteria, yet not so much to mould and yeast. The antimicrobial minimum dosage on different flora actedas 50%, and the pH range of inhibiting bacteria as 3～5. Moreover, the heat stability was good.

The parallel drying experiments of potato were made with both High Electric Drying(HED) and Heat Drying. Thevitamin C retained in experiments were assayed. The results indicated that the drying rate of potato was higher on high electricdying and the vitamin C could be retained more than on heat drying(43.5%) more. The mechanism of high electric drying wasenvisaped. And it was thought that the drying mechanism on high electric field produced the injecting of ion, the effect of unevenelectric field and the formation of ion wind.

Abstact:In the thesis the rheology of trigonella bean gum was studied by studying the apparent viscosity changing affectedby concentration, shearing, temperature, pH, freazing-thawing and synergic effect with xanthan gum. Results showed that theviscosity of trigonella bean gum dissolved in hot-water or cold-water rised exponentially with the rising of concentration; it wasnon-newton fluid and its shear-thinning capability could resile instantaneously, while its viscosity reduced half-logarithmicallywith the rising of temperature and it had strong heat-resistant characteristics; the effect of pH on trigonella bean gum was small;its viscosity reduced with the freezing-thawing; whereas trigonella had little synergy with xanthan gum or Sodiun alginates, itshowed strong synergic effect with Sodiun carboxy methyl cellulose.

Submerged fermentation process and amino acid analysis of Antrodia camphotrata strain were studied. Experimentalresults showed that the composition of the optimal fermentation medium was as fellows:2% Bran, 0.2% Peptone, 0.3% K2HPO4,0.05% MgSO4 and 10mg/100ml VB1. The optimum cultural period was 5 d at 25℃ in 250ml shake flasks at 150r/min. The bestaeration ratio was 12:25 (medium volume: flask volume). The inoculation volume was 15%. The highest dry weight of myceliaof Antrodia camphotrata was obtained (about 1.0g/100ml) in the optimum culture. Comparison of the content of amino acid andpolysaccharide between the mycelia and the filtrate of fermented product was made. It was found that mycelia were equally avery good source for health food.

Zizyphus jujuba MILL was extracted with ethanol and 80～100℃water, the water elution was concentrated at reducepressure, fractions ZJ-A,ZJ-B,ZJ-C were obtained by Fractional precipitation of the water elution with ethanol.And thenprotein was cleared by CCl3COOH. While ZJ-6 was obtained by further purification of ZJ-B with DEAE -Cellulose and Sepchcryls-300. The molecular weight of ZJ-6 was estimated to be 4206 with HPGPC. Spectral and chromatographic analysis (1HNMR,13CNMR,HPLC) were used to investigated the structural characterization of the polysaccharide.Results showed that ZJ-6’mainchain was (1→4)-linked α-D-galacturonic acid ,and it’s side chains included glucose、xylose、arabinose.

The physical and chemical propeties of 3 grain sizes rice powder ground by superfine grinding were comparied withgenerally ground rice powder. The results showed that the smaller the grain diameter distribution, the lower the protein content,the pasted temperature, the dextrin transparency, or the freeze-thaw stability, but the higher the rate of starch hydrolysis withenzyme, the heat stability of dextrin, the cooking character and dissolved solubility, or the larger the stop angle and slide angle.The subsidence and foam-holding capability were improved. It was stable for acid-resisting.

The Oviductus Ranae was used as substrate and hydrolyzed by papain in the study.The optimum enzyme tohydrolyze the Oviductus Ranae was papain. Compared with the Ovidutus Ranae, hydrolysate showed excellent solubility.Thecontent of dissociated oestradiol increased and viscosity dereased with the increasing of the hydrolysis degree.

Three enzymes EI、EII and EIII were purified from crude A.niger inulinase Fructozyme by DEAE-Sepharose CL-6B ion-exchange chromatography.EI was identified as endoinulinase,its molecular weight was 65,000.The optimum reaction pHof endoinulinase was about 4.6 and optimum temperature was 60℃ when inulin was used as substrate. Thus Michaelis constantKm was 16mmol/L and endoinulinase was fairly stable to pH and temperature. The application of endoinulinase was approachedpreliminarily.

The effects of gluten concentration, glycerol concentration, pH of the film-forming solution and temperature of heat-treatment on various film properties were studied by Response Surface Methodology. The results showed that glycerolconcentration, pH and their interactive function had strong effects on tension strength and elongation of protein films. Watervapor permeability of protein films was evidently influenced by glycerol concentration, temperature, gluten concentration andinteractive effects between glycerol concentration and temperature. At the same time, effects of gluten concentration, glycerolconcentration, pH, temperature and their interaction on oxygen permeability of protein films were obvious.

A regression equation was obtained by a central composite design in which the yield of naphthaquinonic compoundsas function of temperature and pressure and its response surface analysis were made.The results showed that regressionequation could anticipate the yield up to 4.1%～4.6% at 27MPa, 35℃ by the figure of response surface.

In this paper , principle model and realization process of ant colony algorithm were reviewed. When the algorithmwas applied in optimization model of Formula of Infant Nutrient Rice Flour the results shows that it was feasible and the algorithmgave a new way for solving similar food formula problems.

Optimization of the solid substrate and culture conditions was a very important measure to decrease production cost,increase enzyme activity and realize industrial production of cellulase. In this experiment, solid fermentation of TrichodermaKoningii F244 in line with uniform design U15(58) had been performed with two different temperatures culture, as 30℃ duringthe first 30 h and at 27℃ in the following hours continuously. Consequently, the activities of FPA, CMCase, cotton lyase andβ-glucosidase were regarded as dependant in Multivariate Regression Analysis in SPSS and then the mathematical model of overallcellulase activity was constructed by adding the corresponding activity equations together with weight. Furthermore, themaximum of cellulase model was figured out by constraint mathematical programming. It revealed that in cellulase production bysolid fermentation with two temperatures culture from Trichoderma Koningii, 72h were proper fermentation time under theconditions of natural aeration, natural substrate pH(about 6.5) and an environmental humidity of 60%. Furthermore, ideal solidsubstrate constituents were rice straw powder 50%, wheat bran 42%, (NH4)2SO4 8% and water about 4.9 time weight of solidconstituents. Moreover, Tween80 in small quantity was helpful for enzyme activity.

A natural red food pigment was extracted from Salvia miltiorrhiza. The constituents were identified by thin-layerchromatography and in comparison with standard substance as 5 Tanshinone: tanshinoneIIA cryptotanshinone dihydrotanshinoneⅠ methylenetanshinquinone and tanshinoneⅠ. Several extraction methods and purification techniques of tanshen red pigmentfrom Salvia miltiorrhiza were compared and selected. The extracting conditions of tanshen red pigment were studied by using theextracting rate, cost, and absorbance as indices with orthogonal test. The results showed that the optimum parameterswere: alcohol reflux extraction three times, 1h at a time, and V(75%alcohol):m(tanshen)=5:1. The optimum purification methodwas:acetidin extraction. These researches provided the academic basis and direction for the use and exploitation of the tanshenred pigment.

Roasted Peach kernels were stored respectively with film and without in the opening air at 36℃. Every other 15d,the contents of amygdalin, oil acid value, POV, CV and moisture were measured . After 60 d the changes of the contents of protein,fat, suger, and iodine value, soap value of fatty oil were also determined respectively in this paper. The results showed that theeffect of preservation with film was better than that without film in the opening air.

The ethanol extract conditions propolis by and their antioxidant activities were studied simultaneously. The resultsshowed the best extract conditions was: ethanol concentration 94.51%, extracting temperature 34.44℃, extracting time 33.86hand ratio of propolis to liquid 1:15.91 by orthogonal test according to Responds Surface Methodology. Anti-oxidative effect ofethanol extract of propolis(EEP)on lard was studied.The results showed that EEP could inhibit the reaction of lipid peroxidationof oil significantly. The anti-oxidative effect of EEP on lard was better than VE and tea polyphenols. Propolis was a potentialproducts of natural ,safe and effective antioxidant.

The object of this paper is to extract mucopolysaccharide from goat cartilage and separate the extraction byDiehlaminoethy so as to determine the optimal extraction and separation technology. In addition, the reason is analyzed in thispaper.

The encapsulation of grape polypheols by chitosan-sodium alginate was studied. Also the controlled release of grapepolyphenols from microcapsules was investigated under the conditions of mimic gastric and intestinal juice. The results showedthat the loading efficiency of microcapsules was mostly affected by the concentration of chitosan. The microcapsulesprepared at pH6.0 exhibited slower release of grape polyphenols than that at pH5.0. The larger the molecular weight ofencapsulated substances, the longer the release time from the chitosan-sodium alginate microcapsules.

The paper studied the extraction of polysaccharide in Schisandra. Single factor test and orthogonal experiment designmethods L9(33) were applied to analyze the influence of the factors such as solid-liquid ratio and temperature and time on theextraction percent of the polysaccharides. Experimental results indicated that temperature and solid-liquid ratio would signifi-cantly affect the extraction percent of the soluble polysaccharides.The optimum solid-liquid ratio was: 1:25, temperature is 100℃ and the extraction time 4h.In this condition, the extraction percent of soluble polysaccharides in Schisandra was 5.38%. Inaddition, the traditional method of polysaccharides precipitated with alcohol was improved. And Sevag method combined withenzyme method based on traditional Sevag method to eliminate protein was adopted. This method greatly decreased the time ofeliminating protein. Also polysaccharide was assayed by improval anthroic ketone-sulphuric acid method.

The cell-disrupting methods, solvent extracting and working conditions for extracting carotenoids form theAzotobacter strainβ-C1 were studied. The method of hot hydrochloric acid was the best to bread the cell wall, and the optimalconditions obtained by orthogonal experiment were: concentration of HCl 3mol/L, HCl addition 30ml/g(dry cell), temperature100℃ and time 2min .Ethanol was the suitable solvent as far as the toxin problem and application convenience were concerned.Besides the optimal conditions were: alcohol addition 30ml/g (dry cell), extracting temperature 50℃ and extracting time 45min.Under the optimal conditions for disrupting cell and extracting carotenoids, the extraction rate was 3.45mg/g.

In this paper the technics about water elute and anhychous alcohol regurgitant methods to distill total flavonoidcompound in Lithocarpus polysachyus rehd were studied. The optimal technics by orthogonal experiment design were applied.Results indicated: the optimal technics was A2B3C2, i.e. , 25 times quantity of the 80% alcohol cinculating 2h.

The preparation approach and quality analysis egg yolk lecithin were discussed in this paper. The 70% rough lecithinfrom fresh egg yolk was extracted three times with ethanol under different conditions and precipitated by acetone. 10% ZnCl2precipitator was used to purify rough lecithin further. About 80% recovery of refined lecithin was obtained, with the content ofphospholipid over 90%. The maximum ultraviolet absorb peak of product occurred at 215nm. The TLC results showed that thelecithin prepared had two clear spots, PC and PE.

Preliminary investigation on processing technology of no sugar Puerariae Radix yoghurt with nutritional and healthyfunctions was reported. The results showed that good quality and white Puerariae Radix juice could be produced by using 0.05%VC+0.1%CA as color protecting agents. Good quality and tasteful Puerariae Radix yoghurt without sugar could be obtained byadopting following conditions: raw materials ratio of Puerariae Radix juice and fresh milk as 3:7, and addition of Aspartame0.05%, gelatin 0.15%, fermentation agent of 1:1 mixture of L.bulgaricus and St.thermophilus with inoculation of 3%, fermentationtemperature 42℃ and fermentation time 5h.

In this study, the four media in quick boiling of potato i.e., Pure water or 1%CH3COOH or 1%NaCl or 1%NaHCO3were assayed respectively by method of single factor experiment. Finally, rotate regression experiment for the boiled potato wascarried out. The target was the VC of potato and crisp. The technology was studied and the optimum conditions were: for NaClmedium 0.3% NaCl, the ratio of potato and media 1 to 3, and boiling time 4.5min;and for CH3COOH medium 0.045%CH3COOH,the ratio between potato and acid 1 to 3, and boiling time 5min. In these conditions, not only the VC of potatowas well protected, but also the edible quality was achieved at a better level. The potato of quick boiling by NaCl or CH3COOHcould be cooked for various dishes.

The comparative effects of different treatment methods applied in Aloe gel juice extraction, viz. freshly crushing,and steam heating-crushing, freezing-crushing, pectinase-added treatments on extraction yield and quality of Aloe gel juice wereinvestigated. It was indicated that the order of juice yield extracted with different methods was pectinse-added>steam heating>freezing>freshly crushing. Compared to the juice squashed from Aloe gel treated with pectinase, the juice crushed from Aloegel treated with steam heating had low nutrient loss, high retention and low viscosity degradation. It suggested that steam heatingwas adaptable to the extraction of Aloe gel juice.

Mashing characteristics of potato used as auxiliary material for beer brewing were studied. The optimum mashingconditions for potato were : adding 30% potato as auxiliary material, and mashing for 60 minutes at 65% after smashed and screenedwith 60-mesh Screen. The resulted wort was confirmed as qualified to the state standard and with higher content of amino acids.The corresponding fermented beer qualily was found to conform to the state standard GB4927.

Using limewater as solvent, rutin was extracted with the operation of microwave. The optimum technology conditionswere obtained as follows: crushing the flower bud scholartree to (0.28mm<d<0.45mm), extracted 2 times by microwave, water-material ratio 1:16, time 10min and power defrost. Then filtered and regulating the filtrate’s pH2～3 with hydrochloric acid, addingsurfactant OP and laid about 30min to deposit completely , filtered and washed with water, dried the residue, was the rutinproduct obtained. The yield was 17.1% and content was about 94.1%. The results demonstrated that less time,less energy andhigher extracting rate by microwave treatment were obtained in comparison with traditional boiling water extraction.

The sausage was made by adding four kinds of the common mashed vegetables(amaranth, carrot, tomato and onion)with the adding proportion of 8%～32% by weight. The color and gelatin properties (hardness, springiness and cohesiveness)of the sausage were examined. The effect of the added mashed vegetables on the sausage quality was also investigated. Theresearch offered the optimum addition for the vegetable sausage.

The effects of emulsifiers Tween 80, Tween 60, TritonX-100, sucrose ester, and SSL on Q9415 fermentation wereexamined respectively both in shake flask and in 30L bioreactor. The addition of Triton X 100 to 30L bioreactor produced28.5g/L exopolysaccharides at 86h and resulted with more viscosious product.

From the single factor and systematical linear tests experiments, we have fixed the optimum extracting conditionfor β-Glucan from oat brans. On the other hand, oatβ-Glucan was purified either by dialysis, ultrafiltration, solventprecipitation, or polyamide column isolation and so on., and the purify result of these methods was compared.

Using two kinds of chromatographic column, Nova-Pak C18 and Bond-Pak C18, the carotenoids in Dunaliella bardawilwere analyzed through high pressure liquid chromatography. The results showed that in the same experimental conditions, theseparation of carotenoids with the Bond-Park C18 was more effective than that of Nova-Pak C18 The qualitative analysis ofcarotenoids to the result of Bond-Park C18 showed that there were over 16 carotenoids in Dunalidlla bardawil. Comparing thechromatograms of D. bardawil with that of D. salina, it was found that, between the two species, the chromatograms showedvery high similarity and the main carotenoids contents were of little difference

Deoxynojirimycin, the physiological active constituents of silkworm (Bombyx mori. L.), was first analyzed domesticall by ion chromatography . By smashing the larva of silkworm , distilling the active constituents by ultrasonic method and injectingit to ion chromatography, four aldaloids were isolated. The relative content of 1- deoxynojirimycin was 0.1890% with areanormalization method . The extraction rate of ultrasonic method was seven times higher than that of the distillation with water.

The organophosphorus pesticide residues of more than 20 kinds of leaf-vegetables, such as those in Cruciferae、Convolvulaceae、Umbelliferae、Composite、Amaranthaceae etc.were studied with FT-NIR for the first time.Methamidophos, the principal materials, and other three kinds of pesticide (representing high、middle、low poisonaccordingly) were investigated. The differences in the spectra of different vegetables were discussed. The organophosphoruscharacteristic radicals in the first overtone、difference technology、derivative pre-treatment etc. were used to decipher theprimary peak.To compare with GC-MS method, FT-NIR proved to be an easy、fast、reliable and practical method in thedeterment of organophosphorus pesticide residues.

A gas Chromatography-electron impact mass spectrometric method was developed for analyzing the compositionof the fatty acids form purslane 2-amino-2-methylpropanol was used as chemical modifying reagent to the fatty acids. Modifyingcarboxyl group into nitrogen-contaning heterocycle effectively suppressed the migration of double bonds in the fatty acid chainunder the electron impact source and led to regular informative mass spectra regarding the position of the olefinic bonds in thechain. The position of double bonds of the fatty acids form purslane was located by analyzing electron impact mass spectra of2-amino-2-methylpropanol derivatives of the fatty acids. 10 fatty acids were identified according to the analysis of GC-EIMS.Purslane consisted of the fatty adids of C16～C22 and 70.64% of them were polyunsaturated fatty acids. The main componentof the polyunsaturated fatty acids was 9,12,15-linolenic acid whose relative content was 50.08. Besides, 5,8,11,14,17-eicosapentaenoic acid(EPA)and 4,710,13,16,19-docosahexenoic acid(DHA)which existed rarely in other plants were identified.

A new method is presented for the determination of tin in food. The method is based on that zinc can be reduced toSn4+ to Sn2+. The reaction of Sn2+ with phosphomolybdate ammonium formed blue substance on certain conditions. Beer’s lawwas obeyed in the range of 0～20g/ml for tin. The detection limit was 0.001g/ml, and the recovery was 96.9%～102.06%.

The contents of amino acids, trace elements and toxic heavy metals were determined in the fruit bodies of Lentinusedodes cultivated with different sawdust-saving cultivation substrates. The nutritional value and security of the fruit bodies ofL.edodes cultivated with different sawdust-saving cultivation substrates were also discussed. The experimental results showedthat both sample1and sample2 contained 17 kinds of amino acids, 8 kinds of trace elements and 4 kinds of toxic heavy metals,but the contents of toxic heavy metals were lower than that of the State Standards on Food Hygiene. Comparison of the contentsof amino acids, trace elements and heavy mentals between sample1and sample2 was also made. It was found that two samplesfor test were equally good and health foods.

To study the antiproliferative effects of isoflavones on gastric cancer cell lines in vitro, we used stomach cancer cellline SGC-7901 as the model in vitro. We adopted MTT, mitotic index experiment and clone formation experiment to study theinhibition effect of isoflavones. Genistein had an prominent antiproliferative effects on stomach cancer cells in dose- and time-dependent. The antiproliferative effects of other isoflavones on stomach cancer cells were very weak. Through the experiment,the antiproliferative effects of various isoflavones were investigated. The antiproliferative effects of genistein were proved. Thestudy results provided the theoretical base for the use and exploitation of isoflavones.

The scavenging effects of Litchi fruit pulp and Longan fruit pulp on O2-• were studied by using ammoniumpersulfate /N,N,N’,N’ tetramethyl ethylene diamine system (AP-TEMED) method. The cleaning capability of polysaccharidesof Euphoria Longan (Lour.) Steud and the Litchi Chinensis Sonn for oxygen free radicals was determined. The results showed thattwo kinds of fruit scavenging effect on activated oxygen were rather strong, and the result of Longan was better that Litchi. Theability of the polysaccharides of Litchi to weeded out the oxygen radical was us with the amount of polysaccharide but Longanhad not the similar ability.

The Propolis’s basic component is the flavonoids,about 20 kinds or so. Besides, it also includes multivitamin, aminoacids, microelements, many active enzymes,spirits,lipids and acids.The experiment showed that in comparison with the miceof the control which were fed on the high lipids, the high dosage proplis could obviously reduce the concentration of TC in theblood serum, and obviously increase the HDL-C/TC ratio. Both of them had the obvious sginificance by the statistics(p<0.05).The effect of proplis on the level of the triglyceride in the blood serum had not been found in the expenriment. According to thejudgement standard of 《The way and procedure of evaluating healthy food function》 it was believed that propolis showedthe regulation effect of the blood lipids.

Water-maze and step-down method were used to test whether theanine would affect Mice’s learning and memorialcapabilities. The test turned out with the results that certain dosage of theanine would shorten the time to reach the final in thewater maze and reduce the mistakes as well. The theanine could also extend the time of memory so as to significantly lessen themistakes. Therefore, the conclusion could be made that the theanine could help the mice to improve their learning and memorialcapabilities.

Object: To evaluate the nature occurred new anti-leukemia candidate drug, apoptosis inducing effect of main ingredient(-)epigalocatechin galtate (EGCG) of tea polyphenols was studied in vitro. Methods: The apoptosis inducing effect of EGCG and theoptimal duration and concentration of its action were observed on the cultured human promyelocytic leukemia HL-60 cell line in vitrounder light and transmission electro-microscopy as well as DNA agarose gel electrophoresis. Result: The cultured HL-60 cells growthwas inhibited and DNA ladder was clearly shown on electrophoresis and the ultrastructure of the cells exhibited significant alterationsafter treated with EGCG. At the concentration of 250μg/ml for 6h showed the most evident apoptosis effect. Conclusion: Thetea ingredient EGCG did induce apoptosis of HL-60 cells in vitro, and might be a candidate of anti-leukemia remedy.

Cold and controlled atmosphere (CA) storage for vitamin C, chlorophyll a, chlorophyll b and firmness were studiedfor broccoli. The optimum storage conditions were: 2℃ and CA with O2 3%, CO2 2% and N2 95%. The broccoli could be keptfresh over 28d and the ratio of sound ones about 84%. The storage temperature effect was tested outweigh the CA conditions.The key to extend the storage of broccoli was to inhibit the bud efflorescing and yellowing.

Fuji”apples were treated with 0℃、CO2>40ml/L and 0℃、CO2<40ml/L respectively with 0℃ storageas control test. The browning index and activities of SOD, POD and CAT, MDA level of the flesh tissue were investigatedrespectively. The results showed that CO2>40ml/L could induce flesh browning. Activities of SOD, POD and CAT decreasednotably and MDA level and relative electrical conductivity increased sharply in the browning fruits during storage.

Litchi fruits were stored at 20℃ after respective treatment with mixed gas of 10%CO+90%N2 and 20%CO+80%N2for 24h. Appearance, color of pericarp, respiration rate and components of nutrition of Litchi fruits were investigated duringstorage to evaluate the effect of high concentration CO2 short time treatment on storage quality of Litchi fruits. The results showedthat both high concentration CO2(10% and 20%) short time treatment could lead to a higher fresh rate of fruits, retaining purerred of pericarp (higher chroma and lower hue angle). Meanwhile CO2 treatment could not affect respiration rate, total sugar, titratableacidity, and vitamin C of Litchi fruits significantly in comparison with CK.

The relationships between the post-harvest ethylene level in Litchi fruits and its rate of respiration and between rindbrowning and its water transport system were investigated. It was found that the maximum amount of ethylene produced duringthe initial period after harvest was 0.195μl/L and then dropped and kept somewhat steady in the range of 0.04～0.09μl/L. Thechange in ethylene level was found to be in good agreement with the rate of respiration. During the later period of the test, partialrind browning occurred, with a slight rise in ethylene level, suggesting it might have something to do with its physiologicaldeterioration. Treatment with ethrel at normal temperature (25～26℃) induced a respiratory climax, while treatment with ethrelat low temperature(13℃) showed an erratic change in respiration. This reaction pattern of Litchi fruits to ethylene was in goodaccordance with that of a typical non-climacteric fruits. Also observed is the special feature that the localized water loss in itsrind was hardly compensated through its water transport system, and thus caused rind browning. Therefore, the primary reasonfor rind browning might be physiological, not pathological. Thus for the storage of septic fruits such as litchi, the physiologicalproblems should be taken into account first and foremost, and then the pathological ones.

The microbial mutagenic molecular mechanism has been increasingly well investigated, thanks to the rapiddevelopment of molecule biology,cell biology and molecule genetics and so on. The paper expoundsed emphatically themolecular mechanism of DNA damage and gene mutation, as well as elucidated varieties of mutagenesis and hereditary effects.And then it enumerated mutants utilized in meat industry.

A probiotics is a live microbial feed supplement that exerts beneficial effects for the host via improvement of themicrobiological balance in the intestine. They have many kinds of other normal gut flora of incomparable physiological functionto organism. The probiotics that use both at home and abroad are lactobacillus and bifidobacteria mainly. At present, foreignprobiotic products are various in styles. Probiotics was developed in Japan earlier and its products have formed commercialscale. Many countries of EU have had varieties of probiotic products. Though USA starts relatively late in this respect, has alreadydeveloped a series of probiotics products rapidly. Our country catch probiotics and its products research of concern were alsorelatively backward in reaearch and rather different in benefit. Hope to have some domains of break-through in the future. In awords, the prospect of probiotic products is surely to be very bright.

This paper introduces the U.S. Meat and Poultry Inspection System from several aspects including acts andregulations, organizations, the controlling measures of germina, the system of HACCP, the Residues Surveillance System,inspection in imports/exports, and puts forward some concrete suggestions aiming at the facts in our country.

With silver carp fish as the raw material, the single factor experiment was combined with orthogonal tests to studymanufacturing of soft can of fragrant silver carp fishes. It was found that 1% sodium chloride showed the best oder removal for60 minutes. The Optimun factors were: deep frying 2～3 minutes under the 170～180℃.Furthermore, to soak in different timefor the flavor, the optimum effect was soaking in 2 minutes. For germs sterilization the formula for the germicidal conditions 20′-30′-20′/121.2℃ were obtained. The product could be kept for a long time, above 12 months.

The new retort pouch food was developed with special flavor, enough nutrition and convenient in eating with the chestnutand pig hoof as the main material and mixed with the soy, cooking wine and fragrant hard material etc. The optimal technical formulaand processing technique were obtained by the orthogonal test, shelling test, good color and fragrance reaction test.

In compounding the gum with right amount the lotus leaf for bacteriostasis, it showed health care function on theoral cavity, due to the green original acid and different green original acid liquid of the Honeysuckle. Having cleared away bodyheat and detoxified efficient component it would increase the health care function of the gum.