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Table of Content

15 April 2002, Volume 23 Issue 4
An Investigation on Inhibition of Lipoxygenase Activity,Related to Ripening and Senescence of Vegetables &Fruits
ZHANG Yin-Liang, MA Qing-Yi, LIU Yuan-Yuan, ZHANG Xun
2002, 23(4):  25-27. 
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Lipoxygenase is a key enzyme in ripening and senescence of vegetables and fruits . Lipoxygenase was extracted from soybean and the inhibition of it’s activity was investigated so as to select more inhibitors with enzymatic kinetic methods in vitro. The results showed that cysteine、 vitamin C 、BHT 、 TBHQ and 95% ethanol extracts of HOUPU、BAISHAO etc had good inhibitory effect on lipoxygenase .
Study on Heterotrophic Growth of Chlorella Ellipsoidea
PAN Xin, LI Jian-Hong, DAI Chuan-Chao, HAO Yun-Tao, MA Yu-Xiang, WANG Xue-Feng
2002, 23(4):  28-33. 
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The optimal condition of Chlorella ellipsoidea L1 strain under heterotrophic culture to get high biomass were: BG-11 medium with 10g/L of glucose as the carbon source and 0.2-1g/L of Urea as the nitrogen source,25~30℃ cultivation temperature, initial pH 5.5~6.5, 100ml medium in 250ml flask and seeding 10%(V/V). By inoculating the pre-culture to a conventional 2L aerated and stirred the minijar ferment containing 1.5L of the medium, we got dry cell weight 5.42g/L. The productivity was about 20 times as much as that for photoautotrophically grown cultures. Compared with phototrophic, the lipids content increased, whereas the protein contents and ashes decreased.
Studies on Preparation and Properties of Hydroxypropylated-Phosphate Monoester Corn Starch
LI Guang-Lei
2002, 23(4):  34-37. 
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In this paper,the properties and the optimum conditions for the proparation of hydroxypropylated- phosphate monoester corn starch were studied. The results showed: the hydroxypropylated-phosphate monoester compound modified corn starch gained the merits of the single modified starches and overcame at the same time their shortcomings and thence forth the degree of transparency, the freeze-thaw stability, the effects of cane sugar and foam-holding capacity were improved.
Study on Extraction Purification and Propertes Analysis of siraitia grosvenorii Root starch
CHEN Quan-Bin, TANG Gui-Mei, YI Xiang-Hui
2002, 23(4):  37-41. 
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In this paper, studies were carried out on the extraction and purification process of starch of Siraitia grosvenorii’s root. Survey was made on the contents of starch and of reducing sugar, the solubility of the starch, the transparency and subsidence of the dextrin. The results showed as follow: the degree of white of the starch was 94.5 and the content of pure starch in the drying root(two years old ) amounted to 30.89%. In this work, the promising future of the development of the starch was predicted.
Identification of Enzymatic Browning Substrates in Fresh-cut Lotus Roots
YU Zhi-Fang, ZHAO You-Xing, LI Ning, XIA Zhi-Hua, PENG Gui-Xia
2002, 23(4):  41-44. 
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The enzymatic browning and its main substrates in fresh-cut lotus roots were studied during storage period. The results were as followsing: The newly synthesized phenolic substances were mainly the FP, which were the primary phenols catalyzed by PPO to cause browning; The changes of free phenols (FP) and browning degree (BD) after 6 days storage had significantly negative relationship (r=0.959); The FP, catechol and PPO activity showed the synchronous change pattern which increased first and decreased afterwards;The content of catechol in Fresh-cut lotus roots measured by thin layer chromatography and HPLC decreased significantly after browning; The absorption spectrums of catalytic product of catechol and browning product in fresh-cut lotus roots are identical, and Catechol therefore was considered as the major browning substrate in fresh-cut lotus roots.
Study on Extration of Flavonoid in Zingiber Officinalis and Determination of Antioxidation Activity
YANG Yang
2002, 23(4):  45-50. 
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The extraction technology for flavonoid in zingiber officinalis was studied, the results showed that the highest extraction rate of flavonoids could be obtained with 80% aqueous ethanol refluexing at a radio of solid to liquid as 1:2 , at temperature 80℃ and vibrating extraction 3h .The stability and antioxidative astivity of zingiber officinalis extract were studied at different conditions.
Probe into Enzyme Method of Pinctada Martensii Hydrolysis
HAO Ji-Ming, ZHANG Jing, CHEN Su-Hua, HONG Peng-Zhi, ZHANG Chao-Hua
2002, 23(4):  51-53. 
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In this paper ,we studied useing proteinase to hydrolyze Pinctada martensii meat. The optimal conditions for hydrolysis by enzyme method were : B.Subctilis neutral proteinase hydrolyzed at pH6.5,temperature 40℃, enzyme consistency 3×103U/ml , K+ concentraction 0.12%,and time 3.0h.Then add stomach proteinase at pH3.0,temperature 60℃, enzyme consistency 4.5U/ml , and time 3.0h, The degree of hydrolysis of mussel meat is 66.2% by this method. The hydrolysis liquid can be used to make high quality seafresh seasoner and fundational protein drink.
Technology Study on Producing Flavor Food From Eucheuma
LI Liu-Dong, LI Lai-Hao, CHEN Pei-Ji, YANG Xian-Qing, DIAO Shi-Qiang, WU Yan-Yan, HUANG Jian-Nan
2002, 23(4):  53-56. 
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The technique and control point on producing flavor food of Eucheuma are introduced in this paper. The intensity and flexibility of this kind of seaweed are increased and senses in mouth are increased when Eucheuma was dipped in water solution of 10% NaOH liquor for 24h. The sauce ingredients of flavor food of Eucheuma are chosen strictly by orthogonal design method. The result in general evaluate of color and savory and sapor shows that the sauce effects are the best for all of the food when the ratio of the soybean sauce : mature vinegar:capsicum oil : pimiento oil is 2.0:3.0:2.0:1.5.that is just an ideal combination for this sauce formula.
Studies on Extraction,Separation and Purification of the Submerged-fermentation Morchella Polysacharide
JIA Jian-Hui, LU Xiao-Lian , FAN Li-Qing
2002, 23(4):  59-63. 
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We have gotten Morchella polysaccharide by submerged fermentation,the main material is from Morchella mycelia and fermented liquid.We have made comparative experiments amond elements which influence extract rate and puried polysaccharide analysed components of polysaccharide.
Study on Herbal Medicinal and Edible Antialcoholism Functional Drink
ZHANG Hui-Xiang, LIU Lin-Wei
2002, 23(4):  63-65. 
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The processing technology for both herbal medicinal and edible antialcoholism functional drink with Hovenia dulci Thunb and Pueraria lobata (Willd) chwias the main raw meterials was introduced in the paper.Selecting the optimum recipe and making animal experiments for antialcoholism function of drink were reborted in the experiment.
Functional Study on Baking Food Enriched in Dietary Fiber and Oligosaccharides
ZHANG Yi-Ming, WANG Ya-Wei, MA Li-Qing
2002, 23(4):  66-69. 
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The selection of the type of the products suitable to be enriched with dietary fiber and oligosacchide,the optimum recipe the processing technology and the enriched amount of dietary fiber were studied.The results showed that the amount and kind of dietary fiber and oligosacchride took a vital effect on the appearance and intrinsic quality of the baking food.
Clarification Study on the Removing Method for Non-biological Precipitate of Thick Wine
LI De-Min, WU Hong, SUN Xin-Ji, GUO An-Ming, WANG Ying-Feng, PU Bin, LIU Lei
2002, 23(4):  70-73. 
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The removing method for non-biological precipitate of thick wine by enzymatic diatomaceous earth bentonite polyvinylpy rrolidone or food gelatin was studied.The results indicated that the filtering effect was very good by combining diatomaceous earth with polyvingylpolyrr-olidone or food gelatin with polyvinylpolyrrolidone.
Technology Study on Acid Hydrolysis of Soybean Isoflavone
LI Wen-Liang, HAN Ji-Fu, CUI Hong-Bin
2002, 23(4):  73-75. 
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The acid hydrolysis of soybean isoflavone sugar-moiety to produce a new soybean isoflavone containing low sugar and high activity daidzin was studied.The optimal reaction conditions of the acid hydrolysis of soybean isoflavone were:0.1mol/L hydrochloric acid for 3 hours,temperature 40℃and the ratio of hydrochloric acid and sample 5∶1.Genistein content was 5.95% and the ratio of break 5.25%.
Study on Supercritical CO2 Continuous Extraction of EPA and DAH from Fish Oil
ZHENG Mei-Yu
2002, 23(4):  76-78. 
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Continuous extraction of EPA and DHA form fish oil by using supercritical carbon dioxide was studied .The effects on separation performance,including flow rate ratio of carbon dioxide to fish oil pressure and temperature were studied.And the technical conditions were researched.
Study on Cellulase Degradation of Soybean Cell wall to Produce Beancurd
YU Shu-Yi, WANG Chuan-Huai
2002, 23(4):  79-82. 
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The effects of utilizing some cellulase,a product being brought by high biological technology were investigated to increase the ratio of swelled beans and the yield of bean curd by steeping in water to degrade the cell walls and extract protein during the process of the production of bean curd .The tested results showed that cellulase and hemicellulase could well degrad of the cell walls when steeping the soybean in water.The most suitable condition was steeping the soybean with 0.1% cellulase at 28℃ during 6h.The pH range of 5~6 was generally suitable.
Study on Glutamine Metabolically Controlled fermentation Technology
WANG Wei-Ping, WU Si-Fang, YANG Jin-Shu, WANG Shi-Hua
2002, 23(4):  82-85. 
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We made an experiment of optimization on the technological conditions of Glutamine metabolic ally controlled fermentation by high production glutamine strain SH77 which was obtained by mutation. Under optimal fermentation technology condition, it was able to accumulate an average of 5.3% and the highest of 5.62% of glutamine.
Clarification Study on Wild Vaccinium Vitisidaea Beverage
LING Chao-Yang, WAN An-Liang
2002, 23(4):  86-88. 
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Clarification effects of four kinds of clarifiers on wild Vaccinium vitisidaea beverage were studied.The results showed that beverage clarifier Ⅱ had the best and fast clarification effects.It was separated easily with large floceulent particle deposit.After being clarified with clarifier Ⅱ the beverage showed high transparency and stability without undesirable effect on its color and flavor.Mean while this clarifying technology was easy processed with low cost.The best dosage was 0.05%.
Technology Study on Vacuum Flexible Packaging of Poly-staros
SHI Xiao-Qiong, DENG Jin-Xing, ZHANG Ying-Bin
2002, 23(4):  89-92. 
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The optimum technological conditions and subsidiary technology on the vacuum flexible packaging of poly-staros were studied.The results showed that the shape of the product was whole,the quality was excellent and the storage life could reach 1 year with the room temperature 20℃~30℃,the content of lactic acid of poly-taroes could reach 0.6% by humid-state fermentation of lactic acid for 5~7 days.Then the vacuum flexible packaging added with 0.05% lemon liquid as juice and adopted the germicidal technological conditions of 10’~30’/100℃ to make normal pressure sterilization.
Studies on the Industrial Process of Producing Kudzu Starch
SUN Qing-Jie, YANG Ming-Yi
2002, 23(4):  93-94. 
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By filtration and centrifugation, the starch of kudzu is recovered. The recovery could be over 90 percent. This is an industry process which could yield the starch over 10 tons per day.
HPLC Simultaneous analysis of mono-saccharide in Pumpkin
ZHOU Hong, YANG Xiang-Liang, ZHANG Xiao-Yu
2002, 23(4):  95-96. 
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Mono-saccharide in pumpkin was simultaneously assayed by HPLC within 8mins ,on a Spherisorb NH2 column by using a differential refract-meter detector and a mobile phase of acetonitrile-water (85:15).RSD of the sample was less than 2.20%.
Study on GC Assay of Benzoyl Peroxide Contentin Wheat Flour
LIN Qiu-Ping
2002, 23(4):  97-98. 
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The benzoyl peroxide in wheat flour was changed into benzioc acid. Benzioc acid was analyzed by gas chromatography method. Such results showed that the calibration curve was in linear relationshipin the range of 0~200μg/ml, whereas the correlation coefficient was 0.9998. The recovery rate was 84.3%~108%. This method was simple、fast and accurate. It was a good way to assay the concentration of benzoy1 peroxide in wheat flour.
Study on Assaying Methods of Meat Elasticity
JIANG Yu-Jian, ZHOU Yan
2002, 23(4):  99-102. 
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The assaying methods of meat elasticity with the high quality equipment,TA-XT2i,were described. The tested data were compared with the sensory values. The results showed that it was suitable to express meat elasticity by the biggest force in one compression movement.
Immunochemistry Assays of Lactoferrin-Enzyme-linked Immunosorbent Assays
LU Rong-Rong, SUN Zhen, XU Shi-Ying, WANG Zhang
2002, 23(4):  102-104. 
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Enzyme-linked immunosorbent assays (ELISA) were developed for lactoferrin determination of bovine colostrum. The detectable concentration was linear from 3.1 ng /mL to 200 ng/mL. Minimal detectable concentration was 1.5 ng /ml. The recovery rate was 91.82%~115.56%.
Study on the Determination Methed of the Flavonoids of Ginkgo Biloba Extract
SHI Hong-Qi, MIAO Jin-Lai, HAN Li, ZHANG Ai-Jun, LU Pei-Ding , SHI Shu-He
2002, 23(4):  105-107. 
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The quantity of Ginkgo Flavonol Glycosides in GBE (Ginkgo Biloba Extract) was determined by FPLC techniques using external standard method. The column was packed with C18 (4.6μm×250mm). The mobile phase consisted of method-phosphate-water(60:5:35).The flow rate was 1.5ml/min and detection was affected at 360nm. It showed that the quantity of sample and the area of peak were in linear relationship from 2μg/ml to180μg/ml. The average recovery was 97.5%. The relative standard deviation was 1.60%. After the hydrolysis of the flavonoids , the content of quercentin decreased, but the content of kaempferol increased and isorhamnetin remained the same level.
Assay of Reducing Sugar in Wine and honey by Diffuse Reflectance Spectroscopy
ZHANG Ping, YAO Yan
2002, 23(4):  108-110. 
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A new method for determination of reducing sugar in wine and honey by Diffuse Reflectance Spectroscopy was described, and the conditions of assay were discussed. The advantages of this method were the simplicity of operation, the sensitivity of determination (the detection limit was 10μl) and the small amount of regents required (<1ml). The amount of reducing sugar in the range of 20~130μg showed a good linear relationship. Using the is method to determine some samples, the results were satisfactory.
Analysis of Essential oil Collected at Night from Cestrum Nocturnum(Cestrum Nocturnum Linn)
CHEN Zhi-Xing, YOU Xin-Kui, ZANG Xiao-Ping
2002, 23(4):  110-112. 
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The essential oil of branch at night from Cestrum nocturnum Linn was analyzed and determined by GC/MS. 49 compounds were obtained and identified in the oil . The main constituents were 1-Heneicosyl formate(11.28%); Docosane(9.70%); 2-mono-Linolein(7.89%); Dibutyl phthalate; 10-Heneicosene(c,f); 1,3-dimethy Benzene; 1,2,3-trimethyl Benzene ; 4-ethyl-1,2-dimethyl Benzene; Tetracosane; 2,4-bis(1,1-dimethylethyl)-Phenol ; 1-ethyl-3-methyl Benzene, etc. Their toral content was 80.98% of the identified volatile compounds.
Assay of Reducing Sugar in Raw Aloe by Two Wavelengths Method
WU Guang-Feng, WU Yan, WU Sheng-Fang, TANG Jian
2002, 23(4):  112-114. 
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The reducing sugar content in raw aloe was assayed by UV at two different wavelengths to avoid the interference of anthracene derivatives.Using this method the reducing sugar content in Curacao Aloe (Aloe vera L).and Chinese Aloe (Aloe vera L.,var.Chinese (Haw)Berger) were detected to be 2.15mg/ml and 2.74mg/ml respectively.
Study on Catalytic Dynamics Photometric method to Assay I2 Content in Salt
TU Jian-Ping
2002, 23(4):  114-117. 
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This paper was based on the catalytic activity of I- in color increasing reaction in which KIO4 oxidiaed in bright green (sf),so as to establishe a new ,high sensitive and high selective method of assaying I2 content in salt by catalytic dynamics photometric method.Linear assaying range was 0.5~12.5μg/25ml and measuring limit was 2.9×10-1μg/ml.Repetition of this method was fine accurate and operation simpler and process prompt.The satisfactory effects were obtained.
Research on MAP Storage of Chike Longan
ZHANG Qing, XIE Jing, XU Shi-Qiong
2002, 23(4):  118-120. 
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This paper discussed MAP storage of Chike longan. The packaging bag material and store temperature were determined, chemical absorption was used to eliminate C2H4 and CO2 ,The bag was washed with N2 to lower the O2 level. The results indicated that compared with cold store, MAP could increase the quality and storage time of longan and that the effect of chemical absorbent was encouraging.
Study on Effects of Cutting Mode and Ca Treatment on Mp Pineapple Quality
PAN Yong-Gui, WANG Guo-Mei, LU Liang-Quan
2002, 23(4):  121-123. 
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The effect of cutting mode and Ca treatment on MP pineapple quality were studied,It is indicated that vertically cutting was beneficial to keeping product’s quality;But effect of Ca on product’s quality was complicated,For vertically cut product,0.4 % CaCl2 was good,While 0.6% was better than 0.4% for across cutting.
Study on Chitosan Coating Effects of Shelly Bamboo Shoots During Storage
HUA Shu-Nan, LI Gong-Guo
2002, 23(4):  123-126. 
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Shelly bamboo shoots were soaked in a solution of chitosan to form a coating on their surfaces. The treated shelly bamboo shoot were stored at 4℃ and 96% RH for 25 days.Weight loss,cellulose,VC and total amino acid were measured during storage.Effect of concentrations of coating agents on shelly bamboo shoot quality was studied and compared.A solution consisting of 1.5% chitosan mixed with 1.0% pethylhy droxy benzoate had a better effect on storing shelly bamboo shoot.
Effects of Anti-browning Treatments on the Sliced Potato and Water Chestnut During Low Temperature Storage
PANG Xue-Qun, ZHANG Zhao-Qi
2002, 23(4):  126-129. 
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The effects of several anti-browning treatments on the minimally processed potato and water chestnut during low temperature storage were studied.The results indicated that sulfur dioxide was still the best color preservative,while phytic acid could almost be a substitute of sulfur dioxide on water chestnut but useless on potato.Vacuum package could inhibit browning of potato and water chestnut to some extent.The activity of PPO and POD of potato was very high,usually 10~20 times higher than water chestnut and thus the browning of potato was typical enzymatic browning.Though the activity of PPO and POD of water chestnut was very low yet it still browned severely,whereas the spectrum of its browning products was significantly different to that of potato.The results impleied that the browning mechanism f potato and water chestnut was different.
Effect of CPP on the Absorption of Calcium in Human Body
CHEN Ya-Fei, CHEN Jin-Xian, ZHU Shao-Juan, XU Wen-Xia
2002, 23(4):  130-132. 
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The effect of CPP on the absorption of calcium in human body Was studied. The results of the test showed that there was a significant increase in urinary calcium of group test (with CPP) than the contros group (without CPP). The enhancement of urinary calcium was 19.7%. It indicated that CPP could raise the absorption of calcium in human body.
Assay Study on Nutritive Components of Pleurotus Eryngii
WANG Feng-Fang
2002, 23(4):  132-135. 
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The contents of ten nutritive components of dry Pleurotus eryngii were assayed. The nutritive components of Pleurotus eryngii and those of Xianggu mushroom, Tremella fuciformis Berk and Auricularia auricular (L. ex Hook) Underw were compared. In Pleurotus eryngii the contents of ash (inorganic salt) and protein were higher, and the contents of mannitol and free amino acids were also abundant. But the contents of fat and total sugar were low. So it was especially suitable for the aged.
An Investigation on Pullution of Mold Fungus in laver for Consumption
MA Qun-Fei, SHA Ji-Hui, LIN Sheng-Qing, JIN Yu-Ling
2002, 23(4):  135-137. 
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Study on the mycoflora in dried products of laver was carried out. Fifty three samples of dried laver were collected from its main producing area on the coastland of Fujian and from the markets. The fungal colony counts were determined on rose bengal agar plates incubated at 27℃ and moulds were classified. Mould was found in all the samples shown 100% positive with total counts ranging from <5~1.2×104CFU/g-1. In the fresh samples collecting from the producing area, fungal quantity was lower and Fusarium was the genus found most frequently. This field mould is the commonly autochthonous flora of plant. The dried lavers obtained at the market stage yielded greater counts. Aspergillus ornatus, A. niger, A. versicolor etc. were the dominan tgroup. They are common storage mould. It was believed that the contamination of these moulds was a result of improper storage, transportation and sale. It suggests a maximun guideline of mould count of 2.0×102 CFU/g-1 in dried products of laver.
Study on Antimicrobial Action of Aloe Extracts
YAO Shu-Min
2002, 23(4):  137-139. 
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The antimicrobial action of a series of the extracts of Aloe could effectively inhibit the growth of bacterium.The anticrobial minimum dlosages of extracts of Aloe were as follows:Staphylococcus aureus 10%,Salmonell a Sp 8%,Escherichia coli 9% ,Enterobacter aerogenes 9% and Bacillus subitlis 11%,The extracts were characterised by their heat stable ability.
Study on Variation of Nutritional Value of Soy Protein Isolate A fter Being Processed into Films
OU Shi-Yi , GUO Qian-Chu
2002, 23(4):  139-142. 
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Pepsin and 2,4,6-trinitrobenzenesulphonic acid were used to test in vitro the soy protein isolate digestibility and the content of reactive lysine of film proteins respectively. The results showed that in vitro protein digestibility and content of reactive lysine of film proteins decreased with increase in pH of film forming solution and addition of cross-linking agents such as tannin, ferulic acid and hydrogen peroxide, also with increase of mechanical properties of films. However, cornstarch seemed an exclusive, which increased the mechanical properties of films but almost kept unchanged in vitro protein digestibility and the content of reactive lysine of film proteins. It can be concluded that the nutritional value of soy protein isolate decreased after it was used to prepare film. Thus, it appeared that it was more reasonable to make films prepared from soy protein isolate as biodegradable films rather than as edible films.
Development and Expectation of Research on Soybean isoflavone
YIN Li-Jun, LI Li-Te, LI Zai-Gui, CHEN Si-Ying-San
2002, 23(4):  152-154. 
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The research on recent development of soybean isoflavones were reviewed. Some reports about isoflavones were analy zed. It showed that processing conditions had large effects on isoflavone glucosidic formation and activities. Fermentation and enzyme engineering could increase the activity of isoflavone. The diversity of gene and environment would lead to different contents of isoflavones in soybean. it would be very significant to develop soybean isoflavone’s product.