Isolation, Purification, and Structural Analysis of Four Functional Saccharides from Chinese Dates
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Crude oligosaccharides were obtained from Chinese dates by hot water extraction, decolorized using (201 × 7) 717 type strongly alkaline anion exchange resin, and purified on DEAE-52 cellulose column to obtain three fractions, JuBO1, JuBO2 and JuBO3. JuBO1 was further purified by Sephadex G-25 column chromatography to obtain two single components, JuBO1-1 and JuBO1-2. Besides, JuBO2 and JuBO3 were desalted by Sephadex G-15 column chromatography, along with JuBO1-1 and JuBO1-2, lyophilized and analyzed by HPLC and GC-MS for monosaccharide composition. The monosaccharide composition consisted of arabinose, mannose, galactose and glucose for JuBO1-1, arabinose, rhamnose, fructose, mannose, galactose and glucose for JuBO1-2, arabinose, fructose and glucose for JuBO2 and arabinose, rhamnose, fructose and glucose for JuBO3. The average molecular weights of JuBO1-1, JuBO1-2, JuBO2 and JuBO3 were determined by gel filtration chromatography to be 5952, 545, 108629 D and 2624 D, respectively.