UPLC-MS/MS检测肉制品中16 种杂环胺前处理方法筛选

摘要/Abstract

摘要： 本文采用串联固相萃取法（Extrelut-PRS-C18固相萃取柱萃取法、PRS-C18固相萃取柱萃取法）、MCX固相萃取柱萃取法和分散固相萃取法（QuECHERS法（Quick、Easy、Cheap、Effective、Rugged、Safe））4种前处理方法对肉制品中16种杂环胺（heterocyclic amines，HAs）进行提取，并从方法学和提取成本角度综合比较了4种方法。结果表明：4种提取方法得到的16种HAs的回收率和精密度及检出限（detection limits，LODs）和定量限（quantification limits，LOQs）存在显著差异，其中QuECHERS法回收率和精密度均优于其他三种方法。MCX固相萃取柱萃取法和QuECHERS法的LODs和LOQs优于其他两种方法；MCX固相萃取柱萃取法和QuECHERS法所需经济和时间成本较低。综合分析，QuECHERS法具有快速，简便，便宜，有效，稳固和安全的特点，有利于肉制品中HAs的准确检测，并值得推广应用。

关键词: 杂环胺, 肉制品, 回收率, 固相萃取, QuECHERS法（Quick、Easy、Cheap、Effective、Rugged、Safe）

Abstract: In this paper, tandem solid phase extraction (Extrelut-PRS-C18 solid phase extraction column extraction method, PRS-C18 solid phase extraction column extraction method), MCX solid phase extraction column extraction method and dispersive solid phase extraction method (QuECHERS method (Quick , Easy, Cheap, Effective, Rugged, Safe)) were used to extract 16 kinds of heterocyclic amines in meat products, and the 4 methods were comprehensively compared in terms of methodology and cost. The results showed that the recovery and precision, detection limits (LODs) and quantification limits (LOQs) of 16 HAs obtained by the 4 extraction methods were significantly different. Compared to the other three methods, QuECHERS method had better recovery and precision. The LODs and LOQs of MCX solid phase extraction column extraction method and QuECHERS method were better than the other two methods; Both MCX and QuECHERS extraction methods showed lower cost and shorter time for 16 kinds of HAs extraction. In summary, QuECHERS method is fast, simple, cheap, effective, stable and safe, which is conducive to the accurate detection for HAs in meat products and is worth to popularized and widely applied.

Key words: Heterocyclic amines, Meat products, Recovery, Solid Phase Extraction, QuECHERS(Quick、Easy、Cheap、Effective、Rugged、Safe)

中图分类号:

TS251.1

丁晓倩惠腾白雪王振宇张德权. UPLC-MS/MS检测肉制品中16 种杂环胺前处理方法筛选[J]. 食品科学, 0, (): 0-0.

雪白 Zhen-Yu WANG. Screening on the extraction methods of 16 kinds of heterocyclic amines detected in meat products by UHPLC-MS/MS[J]. FOOD SCIENCE, 0, (): 0-0.

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