关键词: 微生物组，分析流程，果蔬采后，合成群落，病害防治

Abstract: Uncultured sequencing technology makes it possible to study uncultured microorganisms. This method can not only obtain the information of the species composition of the research object, but also further study the information at the level of functional genes. The commonly used sequencing methods include amplicon sequencing and metagenomic sequencing. A large number of microorganisms are hidden in postharvest fruits and vegetables. But most agri-related microbial research were focused on a relatively small number of pathogens or a single antagonist, so little is known about the diversity and composition of those microbial communities found in agricultural products and the factors that influence their structure. Therefore, we're going to summarize some of the studies that have been done to evaluate the microbial community structure on agricultural products by sequencing, and this paper introduces the definition of microbiome, the analysis process of main sequencing methods and matters needing attention. The application of microbiome sequencing technology in postharvest fruits and vegetables and related research progress were analyzed. On this basis, some possible research directions are put forward to provide some theoretical basis for the research on the microbiome of fruits and vegetables after harvest.

Key words: microbiome, analysis process, postharvest fruit and vegetable, synthesis community, biological control