食品科学

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不同聚合度壳寡糖单体在小鼠体内的吸收分布

王斌1,汪玲2,闫华1,姜启兴2,于沛沛1,夏文水1   

  1. 1. 江南大学食品学院
    2. 江南大学
  • 收稿日期:2021-12-16 修回日期:2022-03-11 出版日期:2022-04-25 发布日期:2022-04-25
  • 通讯作者: 王斌
  • 基金资助:
    国家重点研发计划“蓝色粮仓科技创新”重点专项课题项目;国家自然科学基金青年基金项目

Absorption and distribution of chitosan oligosaccharide monomers with different polymerization degrees in mice

  • Received:2021-12-16 Revised:2022-03-11 Online:2022-04-25 Published:2022-04-25

摘要: 荧光探针标记操作简单并且荧光的检测灵敏度比紫外-可见等传统方法高3~4个数量级,借助荧光探针测定分析不同聚合度壳寡糖单体在小鼠体内的吸收分布。本实验通过制备异硫氰酸荧光素标记的壳寡糖单体,并灌胃给小鼠,借助小动物活体光学成像系统和荧光定量检测,分析聚合度2~5的壳寡糖单体在小鼠体内的吸收分布的差异性。结果显示,荧光标记的壳寡糖单体灌胃给小鼠 1 h后,在小鼠体内的荧光信号达到峰值,壳寡糖能吸收入血,能跨过血脑屏障分布到脑组织中,主要分布在肾和肝脏中,其次分布在心和脾,少量分布在肺和脑;不同聚合度壳寡糖单体在肾和肝的分布量与聚合度呈正相关,而在血清、心、脾、肺和脑中的峰值分布量为壳二糖最多,壳四糖其次,壳五糖最少。明确不同聚合度壳寡糖单体的体内吸收分布的差异性,对于壳寡糖功能活性的构效关系的研究有着一定的指导意义,对于壳寡糖的功能活性机制研究以及加快壳寡糖在食品功能性食品的开发和应用具有重要意义。

关键词: 关键词:壳寡糖, 聚合度, 异硫氰酸荧光素, 吸收分布, 活体成像

Abstract: Fluorescent probe labeling is easy to operate and the detection sensitivity of fluorescence is 3 ~ 4 orders of magnitude higher than traditional methods such as UV Vis. The absorption and distribution of chitosan oligosaccharide (COS) monomers of different degrees of polymerization (DP) in mice is analyzed with the fluorescent probe labeling. In this study, we analyzed the differences in absorption and distribution of COS monomers with DPs of 2~5 in mice by preparing fluorescein isothiocyanate (FITC) labeled COS monomers and gavaging them to mice. Then we detected fluorescence signal by small animal intravital optical imaging system and fluorescence quantification. The results showed that after 1 h of intragastric administration of fluorescently labeled COS monomers to mice, the fluorescence signal in the mice peaked. COS can be absorbed into the blood and can cross the blood-brain barrier to distribute into the brain tissue. COS mainly distributed to the kidney and liver, secondly to the heart and spleen, and a small amount to the lung and brain. The amount of COS monomers distributed in kidney and liver was positively correlated with the DP, while the peak amount of distribution in serum, heart, spleen, lung, and brain was chitobiose>chitotetraose>chitotriose> chitopentose. To clarify the differences in the absorption and distribution of COS monomers with different DPs has certain guiding significance for the study of the structure-activity relationship of COS functional activity. It is of great significance for the study of the functional activity mechanism of COS and accelerating the development and application of COS in food functional food.

Key words: Keywords: Chitosan oligosaccharide, Degree of polymerization, Absorption and distribution, In vivo imaging, Fluorescein isothiocyanate(FITC)

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