鼠李糖脂处理对早酥梨抗黑斑病的诱导作用及其机理

摘要/Abstract

摘要： 为探讨生物源表面活性剂鼠李糖脂对梨果黑斑病的控制效果及其机理，本文以梨果黑斑病菌 Alternaria alternata 为研究对象，研究了鼠李糖脂（Rhamnolipids，RLS）对梨果黑斑病的诱导抗病作用以及其作用机理。结果表明20 mg/mL RLS处理能有效增强早酥梨对A. alternata的诱导抗病性，处理12d后，其病斑直径仅为对照的64.29%。进一步研究发现RLS处理显著提高了梨果组织的苯丙氨酸解氨酶（PAL）、肉桂酸-4-羟化酶（C4H）、4-香豆酸辅酶A连接酶（4CL）和肉桂醇脱氢酶（CAD）的活性，以及总酚和类黄酮的含量；显著诱导了几丁质酶和β-1, 3-葡聚糖酶的活性。提高了NADPH氧化酶（NOX）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）的活性及H2O2和O2.-的含量，同时活性氧清除系统AsA-GSH循环被激活，维持了梨果组织ROS动态平衡，进而降低了梨果细胞膜透率和丙二醛（MDA）的含量。综上表明鼠李糖脂采后处理可通过调节梨果组织苯丙烷代谢、活性氧代谢及病程相关蛋白进而增强其抗病性。

关键词: 梨果黑斑病菌, 鼠李糖脂, 早酥梨, 诱导抗病性

Abstract: In order to evaluate the control effect and mechanism of rhamnolipid on pear black spot. The induction effect of rhamnolipids (RLS) treatment on pear black spot was studied, and possible mechanism was also evaluated. The results showed that 20 mg/mL RLS effectively enhanced the resistance of pear against A. alternata. The lesion diameter of the pear fruit treated with 20 mg/mL RLS was only 64.29% of that of the control 12 d after treatment. Further studies showed that RLS significantly increased the activities of phenylalanine ammonia lyase (PAL)、cinnamate acid 4-hydroxylase (C4H)、4-coumarate coenzyme A ligase (4CL)、and cinnamyl alcohol dehydrogenase (CAD), as well as the contents of total phenols and flavonoids in pear fruit tissue; The activity of chitinase and β-1, 3-glucanase was significantly induced.The activity of NADPH oxidase (NOX), superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and the contents of H2O2 and O2.- were also increased by RLS treatment. At the same time, the reactive oxygen scavenging system ASA-GSH cycle was activated to maintain the ROS dynamic balance state of pear tissue, and then reduced the membrane permeability and malondialdehyde (MDA) content of pear tissue. The results suggested that rhamnolipid post-harvest treatment might enhance disease resistance by regulating phenylpropane metabolism, reactive oxygen metabolism and pathogenesis related proteins in pear fruit tissue.

Key words: Alternaria alternata, rhamnolipids, ‘Zaosu’ pear, Induced disease resistance

中图分类号:

TS255.36

卢玉慧李永才谭芸秀王小晶毕阳. 鼠李糖脂处理对早酥梨抗黑斑病的诱导作用及其机理[J]. 食品科学, 0, (): 0-0.

Yu-Hui LU LI Yong-cai Yang BI. Induction effect of rhamnolipids treatment against black spot of pear fruit and possible mechanisms involved[J]. FOOD SCIENCE, 0, (): 0-0.

参考文献

[1]张鑫楠, 孙平平, 杜艳民, 等. ‘早酥’梨及其芽变果实品质、酚类组分和色素合成基因表达分析[J]. 果树学报, 2020, 37(12): 1787-1797. DOI: 10.13925/j.cnki.gsxb.20200225.
[2]张山林, 陈秀蓉. 早酥梨叶枯病危害损失与防治指标研究[J]. 甘肃林业科技, 2000(02): 14-18. DOI: 10.3969/j.issn.1006-0960.2000.02.004.
[3]Pétriacq P, López A, Luna E . Fruit decay to diseases: can induced resistance and priming help?[J]. Plants, 2018, 7(4): 77. DOI: 10.3390/plants7040077.
[4]Romanazzi G, Sanzani S M, Bi Y, et al. Induced resistance to control postharvest decay of fruit and vegetables[J]. Postharvest Biology & Technology, 2016:82-94. DOI: 10.1016/j.postharvbio.2016.08.003.
[5]Vatsa P, Sanchez L, Clement C, et al. Rhamnolipid biosurfactants as new players in animal and plant defense against microbes[J]. International Journal of Molecular Sciences, 2010, 11(12):5095-5108. DOI: 10.3390/ijms11125095.
[6]Varnier A L, Sanchez L, Vatsa P, et al. Bacterial rhamnolipids are novel MAMPs conferring resistance to Botrytis cinerea in grapevine[J]. Plant, Cell & Environment, 2009, 32(2):178-193. DOI: 10.1111/j.1365-3040.2008.01911.x.
[7]Sanchez L, Courteaux B, Hubert J, et al. Rhamnolipids elicit defense responses and induce disease resistance against biotrophic, hemibiotrophic, and necrotrophic pathogens that require different signaling pathways in Arabidopsis and highlight a central role for salicylic acid[J]. Plant physiology, 2012, 160(3):1630-1641. DOI: 10.1104/pp.112.201913.
[8]Duan X W, Liu T, Zhang D, et al. Effect of pure oxygen atmosphere on antioxidant enzyme and antioxidant activity of harvested litchi fruit during storage[J]. Food Research International, 2011, 44(7):1905-191. DOI: 10.1016/j.foodres.2010.10.027.
[9]Mondal K, Sharma N S, Malhotra S P, et al. Antioxidant systems in ripening tomato fruits[J]. Biologia Plantarum, 2004, 48(1):49-53. DOI: 10.1023/B:BIOP.0000024274.43874.5b.
[10]Mittler R. Oxidative stress, antioxidants and stress tolerance[J]. Trends in Plant Science, 2002, 7(9):405-410. DOI: 10.1016/s1360-1385(02)02312-9.
[11]Jimenez A, Creissen G, Kular B, et al. Changes in oxidative processes and components of the antioxidant system during tomato fruit ripening[J]. Planta, 2002, 214(5):751-758. DOI: 10.1007/s004250100667.
[12]Shang Q M, Liang L, Dong C J. Multiple tandem duplication of the phenylalanine ammonia-lyase genes in Cucumis sativus L[J]. Planta, 2012, 236(4): 1093-1105. DOI: 10.1007/s00425-012-1659-1.
[13]Zhou F, Xu D, Liu C, et al. Ascorbic acid treatment inhibits wound healing of fresh-cut potato strips by controlling phenylpropanoid metabolism[J]. Postharvest Biology and Technology, 2021, 181(12): 111644. DOI: 10.1016/j.postharvbio.2021.111644.
[14]Knobloch K H, Hahlbrock K. 4-Coumarate:CoA ligase from cell suspension cultures of Petroselinum hortense Hoffm. Partial purification, substrate specificity, and further properties[J]. Archives of Biochemistry & Biophysics, 1977, 184(1): 237-248. DOI: 10.1016/0003-9861(77)90347-2.
[15]Li H, Suo J, Han Y, et al. The effect of 1-methylcyclopropene, methyl jasmonate and methyl salicylate on lignin accumulation and gene expression in postharvest 'Xuxiang' kiwifruit during cold storage[J]. Postharvest Biology and Technology, 2017, 124: 107-118. DOI: 10.1016/j.postharvbio.2016.10.003.
[16]Scalbert A, Monties B, Janin G. Tannins in wood: comparison of different estimation methods[J]. Journal of Agricultural and Food Chemistry, 1989, 37(5): 4245-4245. DOI: 10.1021/jf00089a026.
[17]Cvek J, Medi?-Sari? M, Jasprica I, et al. Optimisation of an extraction procedure and chemical characterization of Croatian propolis tinctures[J]. Phytochemical Analysis, 2010, 18(5): 451-459. DOI: 10.1002/pca.1001.
[18]Zhu L Q, Zhou J, Zhu S H, et al. Inhibition of browning on the surface of peach slices by short-term exposure to nitric oxide and ascorbic acid[J]. Food Chemistry, 2009, 114( 1):174-179. DOI: 10.1016/j.foodchem.2008.09.036.
[19]Sun J, You X R, Li L, et al. Effects of a phospholipase D inhibitor on postharvest enzymatic browning and oxidative stress of litchi fruit[J]. Postharvest Biology & Technology, 2011, 62(3):288-294. DOI: 10.1016/j.postharvbio.2011.07.001.
[20]Fluhr S R. Superoxide production by plant homologues of the gp91phoxNADPH oxidase. modulation of activity by calcium and by tobacco mosaic virus infection[J]. Plant Physiology, 2001, 126(3):1281-1290. DOI: 10.2307/4279996.
[21]Bewley R. Lipid peroxidation associated with accelerated aging of soybean Axes[J]. Plant Physiology, 1980, 65(2):245-248. DOI: 10.1104/pp.65.2.245.
[22]Gao H J, Yang H Y, Bai J P, et al. Ultrastructural and physiological responses of potato (Solanum tuberosum L.) plantlets to gradient saline stress[J]. Frontiers in Plant Science, 2015, 13(5):787. DOI: 10.3389/fpls.2014.00787.
[23]Venisse J S, Gullner G, Brisset M N. Evidence for the involvement of an oxidative stress in the initiation of infection of pear by Erwinia amylovora[J]. Plant Physiology, 2001, 125(4):2164-2172. DOI: 10.1104/pp.125.4.2164.
[24]Bao G, Bi Y, Li Y, et al. Overproduction of reactive oxygen species involved in the pathogenicity of Fusarium in potato tubers[J]. Physiological & Molecular Plant Pathology, 2014, 86:35-42. DOI: 10.1016/j.pmpp.2014.01.004.
[25]Cao S, Hu Z, Zheng Y, et al. Effect of BTH on antioxidant enzymes, radical-scavenging activity and decay in strawberry fruit[J]. Food Chemistry, 2011, 125(1):145-149. DOI: 10.1016/j.foodchem.2010.08.051.
[26]Wei M L, Ge Y H, Li C Y, et al. G6PDH regulated NADPH production and reactive oxygen species metabolism to enhance disease resistance against blue mold in apple fruit by acibenzolar-S-methyl[J]. Postharvest Biology and Technology, 2019, 148:228-235. DOI: 10.1016/j.postharvbio.2018.05.017.
[27]Yu X Z, Zhang X H, Yue D M. Alternation of antioxidative enzyme gene expression in rice seedlings exposed to methylene blue[J]. Environmental Science & Pollution Research, 2014, 21(24):14014-14022. DOI: 10.1007/s11356-014-3306-9.
[28]Marangoni A G, Palma T, Stanley D W. Membrane effects in postharvest physiology[J]. Postharvest Biology and Technology, 1996, 7(3):193-217. DOI: 10.1016/0925-5214(95)00042-9.
[29]HAN X Y, MAO L C, LU W J, et al. Abscisic acid induces differential expression of genes involved in wound-induced suberization in postharvest tomato fruit[J]. Journal of Integrative Agriculture, 2018, 17(12):2670-2682. DOI: 10.1016/S2095-3119(18)62142-2.
[30]Patil S V, Kumudini B S. Seed priming induced blast disease resistance in finger millet plants through phenylpropanoid metabolic pathway[J]. Physiological and Molecular Plant Pathology, 2019, 108:101428-101428. DOI: 10.1016/j.pmpp.2019.101428.
[31]Liu H, Jiang W, Yang B, et al. Postharvest BTH treatment induces resistance of peach (Prunus persica L. cv. Jiubao) fruit to infection by Penicillium expansum and enhances activity of fruit defense mechanisms[J]. Postharvest Biology & Technology, 2005, 35(3):263-269. DOI: 10.1016/j.postharvbio.2004.08.006.
[32]Bhardwaj Garima, Ravi Indu, Kumar Sanjay,et al. Induction of β-1,3-glucanase and chitinase activity, cloning and their characterization in the defense response ofCuminum cyminum plant against the fungal pathogen Fusarium oxysporum[J]. Archives of Phytopathology and Plant Protection, 2021, 54(19-20):1617-1632. DOI: 10.1080/03235408.2021.1932016.
[33]Loon L V, Rep M, Pieterse C. Significance of inducible defense-related proteins in infected plants[J]. Annual Review of Phytopathology, 2006, 44(1):135-162. DOI: 10.1146/annurev.phyto.44.070505.143425.
[34]Mauch F, Mauch-Mani B, Boller T. Antifungal hydrolases in pea tissue:II. inhibition of fungal growth by combinations of chitinase and beta-1,3-glucanase[J]. Plant Physiology, 1988, 88(3):936-942. DOI: 10.1104/pp.88.3.936.
[35]Bernard Fritig, Thierry Heitz, Michel Legrand. Antimicrobial proteins in induced plant defense[J]. Current Opinion in Immunology,1998,10(1):16-22. DOI: 10.1016/S0952-7915(98)80025-3.
[36]刘菊. 鼠李糖脂对西瓜枯萎病防治作用的研究[D]. 浙江大学, 2012.
[37]Conrath, Beckers, Gerold JM, et al. Priming for enhanced defense[J]. Annual Review of Phytopathology, 2015, 53:97-119. DOI: 10.1146/annurev-phyto-080614-120132.
[38]Nukuntornprakit O A, Chanjirakul K, Doorn W V, et al. Chilling injury in pineapple fruit: fatty acid composition and antioxidant metabolism[J]. Postharvest Biology & Technology, 2015, 99:20-26. DOI: 10.1016/j.postharvbio.2014.07.010.
[39]南海风, 朱冠宇, 樊丽, 等. 草莓果实采后衰老过程中活性氧及保护酶活性的变化[J]. 北方园艺, 2016(15): 123-126. DOI: 10.11937/bfyy.201615030.
[40]周春华, 刘红霞, 韦军. 活性氧与果实成熟衰老[J]. 上海交通大学学报(农业科学版), 2002, (01):77-84. DOI: 10.3969/j.issn.1671-9964.2002.01.018.
[41]孔静, 易美君, 许昕, 等. 冷藏期间苹果果实能量和NADP-苹果酸酶及基因表达的变化[J]. 食品工业科技, 2017, 38(11):329-333+339. DOI: 10.13386/j.issn1002-0306.2017.11.055.
[42]李辉, 林毅雄, 林河通, 等. 1-MCP延缓采后‘油木奈’果实衰老及其与能量代谢的关系[J]. 现代食品科技, 2015, 31(04):121-127. DOI: CNKI:SUN:GZSP.0.2015-04-020.