食品科学

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蓝靛果硒多糖的理化性质、结构表征及红细胞氧化损伤保护活性

徐雅琴,邵春天,耿荧,郑秀雯,王茹娟,杨昱   

  1. 东北农业大学文理学院
  • 收稿日期:2022-07-11 修回日期:2022-09-03 出版日期:2022-09-27 发布日期:2022-09-27
  • 通讯作者: 杨昱
  • 基金资助:
    国家自然科学基金资助项目;黑龙江省自然科学基金项目;东北农业大学大学生创新创业计划项目

Physicochemical Properties, Structural Characterization and Protective Effects on Erythrocyte Oxidative Damage of Selenized Lonicera caerulea L. Polysaccharide .

2, 2, 2, 2,   

  • Received:2022-07-11 Revised:2022-09-03 Online:2022-09-27 Published:2022-09-27

摘要: 以蓝靛果果实多糖为原料,采用微波辅助HNO3 - Na2SeO3法制备蓝靛果硒多糖,后经柱色谱分离后得到高含量硒多糖(purification selenized Lonicera caerulea L. polysaccharid, PSLP)。PSLP硒含量为(0.184 ±0.03)mg/g,分子量为58828.3 kDa,由半乳糖醛酸、鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖六种单糖组成,其物质量比为1:3.06:6.18:0.29:1.78:13.01。核磁分析表明PSLP中存在六种糖苷键,分别为:→3)-β-D-半乳糖(1→、→4)-β-D-甘露糖-(1→、→6)-α-D-葡萄糖-(1→、→4)-α-D-半乳糖醛酸-(1→、→2,4)-α-L-鼠李糖-(1→、α-L-阿拉伯糖-(1→。红细胞氧化损伤保护试验结果表明:同H2O2处理红细胞损伤组相比,当保护组PSLP质量浓度为10.0 mg/mL,H2O2诱导的红细胞氧化溶血率降低(32.52 ± 1.23)%;活性氧(reactive oxygen species,ROS)水平降低(44.93 ± 1.88)%;丙二醛(malondialdehyde, MDA)含量减少17.04 ± 0.86 nmol/mL;超氧化物歧化酶(superoxide?dismutase,SOD)、过氧化氢酶(catalase, CAT)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)分别降低20.07 ±1.35 mU/mg蛋白、9.95 ± 0.56 mU/mg蛋白、163.93 ±2.16 mU/mg蛋白。可见蓝靛果硒多糖对红细胞氧化损伤具有一定的保护作用。

关键词: 蓝靛果, 硒化多糖, 理化性质, 结构特征, 红细胞损伤保护

Abstract: The?selenized Lonicera caerulea L. polysaccharide (PSLP) with high content was obtained by microwave assisted HNO3–Na2SeO3 method and chromatography column separation. The selenium content of PSLP was (0.184 ±0.03) mg/g, molecular weight was 58828.3 kDa, and PSLP was composed of galacturonic acid, rhamnose, arabinose, mannose, glucose and galactose with a molar ratio of 1:3.06:6.18:0.29:1.78:13.01. Nuclear magnetic analysis showed that the six glycosidic bonds in PSLP were →3)-β-D-galactose-(1→, →4)-β-D-mannose-(1→, →6)-α-D-glucose-(1→, →4)-α-D-galacturonic acid-(1→, →2,4)-α-L-rhamnose-(1→ and α-L-arabinose-(1→, respectively. Erythrocyte oxidative damage protection test results showed that, compared with red blood cell injury group treated with H2O2, when the concentration of PSLP was 10.0 mg/mL, the erythrocyte hemolysis rate decreased (32.52 ± 1.23)%, reactive oxygen species (ROS) level decreased (44.93 ± 1.88)%, malondialdehyde (MDA) content decreased 17.04 ± 0.86 nmol/mL. In addition, the contents of superoxide?dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px)?decreased 20.07 ± 1.35 mU/mg, 9.95 ± 0.56 mU/mg protein, 163.93 ± 2.16 mU/mg protein respectively. The present conclusions showed that se-polysaccharides from Lonicera caerulea L. fruits had certain protective effect on erythrocyte oxidative damage

Key words: Lonicera caerulea L., selenized polysaccharide, physicochemical properties, structure feature, erythrocyte hemolysis protection

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