食品科学

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脱色对百蕊草多糖的结构及应用特性的影响:H2O2脱色和活性炭脱色的比较

邵丽君1,琚飞龙2,钟杨3,周颖娣1,李贝贝3,凌依凡1,孙玥3,梁进3,李雪玲1   

  1. 1. 安徽农业大学
    2. 安徽省合肥市安徽农业大学中西部大楼246
    3. 安徽农业大学茶与食品科技学院
  • 收稿日期:2022-07-13 修回日期:2022-08-31 出版日期:2022-09-27 发布日期:2022-09-27
  • 通讯作者: 李雪玲
  • 基金资助:
    安徽省中央引导地方发展专项;安徽省自然科学基金;安徽省教育厅科研项目

Effect of decolorization treatment on the structure and application properties of polysaccharides from Thesium chinense Turcz.: Comparison of activated carbon and hydrogen peroxide decolorization

Fei-Long JU 2, 2, 2, 2, jin liang   

  • Received:2022-07-13 Revised:2022-08-31 Online:2022-09-27 Published:2022-09-27

摘要: 本研究采用过氧化氢氧化和活性炭吸附对百蕊草多糖进行脱色得到HTP(过氧化氢脱色处理)和CTP(活性炭脱色处理)两种样品,采用现代仪器手段(X-衍射、热重分析仪、激光粒度仪、流变仪)分析二者的物理特性,并比较CTP和HTP抑制肝癌细胞增殖活性,及其对卡拉胶凝胶温度的影响。实验结果表明,CTP在X-衍射图谱中表现出更加明显的晶体结构;在相同浓度的情况下其表观粘度也高于HTP;热重曲线表明,HTP热分解程度高于CTP(失重率:HTP 59.33%,CTP 53.21%);当CTP浓度达到一定浓度(≥7mg/mL)就表现出抑制人肝癌细胞株HepG2增殖的活性,而HTP在实验浓度范围内(1-10mg/mL)没有表现出抑制癌细胞增殖活性;同时,CTP对文中复配胶的温度滞后增加幅度大于HTP。因此,不同脱色方法会影响百蕊草多糖的结构及应用特性。

关键词: 百蕊草多糖, 脱色, 结构特性, 卡拉胶, HepG2

Abstract: In this study, two polysaccharides from Thesium chinense Turcz. were obtained, with hydrogen peroxide decolorized polysaccharide named HTP and activated carbon decolorizing polysaccharide named CTP. The physical properties of CTP and HTP were analyzed by XRD, thermogravimetric analyzer, laser particle size analyzer and rheometer, and the inhibition of CTP and HTP on liver cancer cells (HepG2) and their effects on the temperature of carrageenan gel were compared.?The results showed that the crystal structure of CTP was more obvious in the XRD pattern, and its apparent viscosity was also higher than HTP at the same concentration. The curve of thermal weight showed that the HTP thermal decomposition degree was higher than the CTP, where the HTP weight loss rate was 59.33%, while the CTP weight loss rate was 53.21%. When the CTP concentration reaches a certain concentration (≥7mg/mL), it showed the activity of inhibiting the proliferation of human hepatoma cell line HepG2, while HTP did not show in the experimental concentration range (1-10mg/mL). At the same time, the temperature hysteresis increase of CTP on the compound gel in the text was greater than that of HTP. Therefore, different decolorization methods can affect the structure and application characteristics of the polysaccharide?from Thesium chinense Turcz.

Key words: Thesium chinense Turcz. Polysaccharide, decolorization, structural characteristics, carrageenan, HepG2

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