HS-SPME-GC-Orbitrap-MS法测定果酒中的醛酮类和呋喃类化合物

摘要/Abstract

摘要： 醛酮类和呋喃类化合物是果酒的重要潜在呈香组分。本文采用顶空固相微萃取气相色谱静电场轨道阱质谱联用法（Headspace solid phase microextraction extraction gas chromatography-orbitrap-mass spectrometry，HS-SPME-GC-Orbitrap-MS）建立了同时测定果酒中5种醛酮类和4种呋喃类化合物的分析方法。通过模拟酒溶液以及枸杞酒、蓝莓酒和山楂酒中检验表明，上述化合物检出限(limits of detection, LOD)在0.004～6.300 μg/L之间，定量限(limits of quantification, LOQ)落在0.01～21.000 μg/L之间，加标回收率均在81%～120%之间，精密度（relative standard deviation, RSD）＜19.08%，该方法准确度高，能够满足果酒中醛酮类和呋喃类化合物定性定量的要求。

关键词: 果酒, 醛酮类化合物, 呋喃类化合物, 顶空固相微萃取, 气相色谱-静电场轨道阱高分辨质谱

Abstract: Aldehydes and ketones and furans are important aroma compounds of fruit wine. In this study, Headspace solid phase microextraction extraction gas chromatography-orbitrap-mass spectrometry (HS-SPME-GC-Orbitrap-MS) was used to establish a method for the simultaneous determination of 5 aldehydes and ketones and 4 furans in fruit wines. The method was validated in model wine, goji berry wine, blueberry wine and hawthorn wine. The obtained limits of detection (LODs) for all target compounds ranged from 0.004～6.300 μg/L, while the limits of quantification (LOQs) were between 0.01～21.000 μg/L. The relative recoveries of all compounds in the three types of wine were between 81% and 120% and the relative standard deviations for precision were all less than 19.08%. In a word, this method meets the requirements for the quantification of aldehydes, ketones and furans existing in fruit wines.

Key words: fruit wine, aldehydes and ketones, furans, headspace-solid-phase microextraction (HS-SPME), gas chromatography-orbitrap mass spectrometry (GC-Orbitrap-MS)

中图分类号:

TS207.3

王磊王春光刘雅冉谷佩珊刘佳妮王丽卫朱保庆. HS-SPME-GC-Orbitrap-MS法测定果酒中的醛酮类和呋喃类化合物[J]. 食品科学, 0, (): 0-0.

磊王 qing BaoZhu. Accurate determination of aldehydes, ketones and furans in nongrape wines through headspace-solid-phase microextraction (HS-SPME) combined with high-resolution gas chromatography-orbitrap mass spectrometry (GC-Orbitrap-MS)[J]. FOOD SCIENCE, 0, (): 0-0.

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