快速滤过型净化结合液相色谱-蒸发光散射法检测茶叶中5种糖类成分

摘要/Abstract

摘要： 首次采用聚酰胺粉作为净化吸附剂材料，基于快速滤过型净化（Multi-plug filtration cleanup，m-PFC）前处理方法，开发了适用于茶叶样品的专用前处理净化小柱，结合高效液相色谱-蒸发光散射法建立了同时检测茶叶中5种糖类成分的分析方法。茶叶样品加水振荡提取，加入二氯甲烷反萃杂质，取上层溶液经m-PFC柱净化，高效液相色谱法测定，外标法定量。结果表明鼠李糖、果糖、葡萄糖、蔗糖、麦芽糖等5种糖类成分在各自相应线性范围内，峰面积的对数与其质量浓度的对数线性关系良好，线性相关系数大于0.99，检出限范围为0.04~0.1 g/100g，定量限范围为0.10~0.20 g/100g。低、中、高三水平的平均添加回收率为91.0%~103%，相对标准偏差为1.0%~4.6%。本方法操作简便，净化效果好，适合茶叶中5种糖类成分的定量检测。

关键词: 快速滤过型净化, 茶叶, 糖, 液相色谱-蒸发光散射法

Abstract: Polyamide powder was used as purification sorbent in the method of multi-plug filtration cleanup method (m-PFC) for the first time. A method was established for simultaneous detection of 5 saccharides in tea by m-PFC method combined with high performance liquid chromatography-evaporative light scattering detector. The saccharides in tea samples were extracted with water by shaking, and then dichloromethane was added to extract impurities. The supernatant was purified by an m-PFC column and determined by high performance liquid chromatography. The accurate quantification was performed by the external standard method. The results showed that the peak area logarithm was linearly related to the concentration logarithm of 5 carbohydrate components, including rhamnose, fructose, glucose, sucrose, and maltose within their respective linear ranges. The linear correlation coefficients were higher than 0.99. The detection limits were in the range of 0.04~0.1 g/100g, and the quantitation limits were 0.10~0.20 g/100g. The average recoveries at three spiked levels were in the range of 91.0%~103%, with relative standard deviations (RSDs) of 1.0%~4.6%. This method has the advantages of simple operation and good purification effect, and is suitable for the quantitation of 5 saccharides in tea.

Key words: multi-plug filtration cleanup, tea, saccharides, Evaporative light scattering detector-high performance liquid chromatography

中图分类号:

TS207.3

董晓倩白春蓉兰韬田旭祁娟王翠丁洁刘松南. 快速滤过型净化结合液相色谱-蒸发光散射法检测茶叶中5种糖类成分[J]. 食品科学, 0, (): 0-0.

Xiao-qian DONG. Determination of 5 Kinds of Saccharides in Tea by Multi-Plug Filtration Cleanup Method Combined with High Performance Liquid Chromatography-Evaporative Light Scattering Detector[J]. FOOD SCIENCE, 0, (): 0-0.

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