基于磁性Fe3O4纳米材料的电化学传感器法快速测定烤肉中的苯并(a)芘

摘要/Abstract

摘要： 本文以磁性四氧化三铁(Fe3O4)、羧基化多壁碳纳米管(MWCNTs-COOH)和Nafion为复合修饰材料，制备了Fe3O4/MWCNTs-COOH/Nafion/GCE电化学传感器，建立了一种快速而灵敏测定烧烤肉制品中苯并(a)芘(BaP)的电化学分析方法。通过循环伏安法对传感器的性能进行表征，研究BaP在不同电极上的电化学行为，并且利用线性扫描伏安法对烧烤肉制品中BaP进行检测。优化结果如下：电解质溶液为磷酸氢二钠-磷酸二氢钾溶液，pH=2.5，最佳分散剂为0.5 %的Nafion溶液，修饰液修饰量为6 μL(2 mg/ml)、富集时间为15 min。在最优条件下，研究BaP浓度对其峰电流的影响，二者在0~100 nmol/L范围内具有良好的线性关系，相关系数 R2=0.9970，检出限为3.12×10?10 mol/L (S/N=3)。该电化学传感器稳定性和重现性较好，利用该法对烧烤五花肉样品中BaP进行检测，加标回收率为86.65 %~96.97 %。可以用于烧烤肉类食品中BaP快速检测。

关键词: 关键词：苯并(a)芘, 食品安全, 电化学分析方法, 肉类食品, 快速检测

Abstract: In this paper, Fe3O4/MWCNTs-COOH/GCE electrochemical sensors were prepared using magnetic ferric oxide (Fe3O4), multi-walled carbon nanotubes (MWCNTs-COOH) and Nafion as composite modification materials, and an electrochemical analysis method for the rapid and sensitive determination of benzo(a) pyrene in barbecue meat products was established. The performance of the sensor was characterized by cyclic voltammetry, the electrochemical behavior of BaP on different electrodes was studied, and benzo(a)pyrene in grilled meat products was detected by linear scanning voltammetry. The optimization results are as follows: the electrolyte solution is disodium hydrogen phosphate-potassium dihydrogen phosphate solution, pH= 2.5, and the optimal dispersant is 0.5% Nafion solution, the modification amount is 6 μL (2 mg/ml), and the enrichment time is 15min. Under optimal conditions, the effect of benzo(a)pyrene concentration on its peak current was studied, and the two had a good linear relationship in the range of 0 to 100 nmol/L, the correlation coefficient R2=0.9970, and the detection limit was 3.12×10-10mol/L (S/N=3). The electrochemical sensor has good stability and reproducibility, and the benzo(a)pyrene in the grilled pork belly sample was detected by this method, and the standard recovery rate was 86.65%~96.97%. It can be used for rapid detection of benzo(a)pyrene in grilled meat products.

Key words: Keywords: benzo(a)pyrene, Food safety, Electrochemical analysis methods, Meat food, Rapid detection

中图分类号:

TS251.7

田野宋紫薇李书国. 基于磁性Fe3O4纳米材料的电化学传感器法快速测定烤肉中的苯并(a)芘[J]. 食品科学.

宋紫薇宋 LI Shu-Guo. Preparation of Nano Electrochemical Sensors for Rapid Determination of Benzo(a)pyrene in Roasted Meat Based on Magnetic Fe3O4 /MWCNT-COOH/Nafion Composite Materials[J]. FOOD SCIENCE.

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