基于复合凝聚法制备红曲色素微胶囊及其稳定性表征

摘要/Abstract

摘要： 为了提高红曲色素的稳定性，采用明胶和阿拉伯胶作为壁材，辅之以谷氨酰胺转氨酶（TG酶）和羟丙基-β-环糊精，通过复合凝聚法制备了红曲色素微胶囊。利用zeta电位、浊度测定确定复合凝聚的工艺条件为明胶与阿拉伯胶的最佳比例为1:1、反应最适pH为4.0。在此基础上，以红曲色素包埋效率为响应值，通过响应面法优化微胶囊制备工艺条件为壁材浓度1.5%（w/v）、芯壁比2.89、凝聚时间42 min，在此条件下红曲色素的包埋效率为85.06%。对红曲色素微胶囊的溶解性、贮藏稳定性、光稳定性及体外模拟胃肠道释放特性进行了表征，结果显示：微胶囊显著提高了红曲色素的水溶性和贮藏稳定性，且在低温及室温条件下贮藏最为稳定；通过模拟红曲色素及其微胶囊的光降解过程，发现红曲红色素和橙色素符合一级动力学模型，黄色素符合零级动力学模型，微胶囊能显著提高红曲色素的光稳定性；微胶囊体系对延缓红曲色素在模拟胃液中的释放有良好的效果，在模拟肠液中的释放速度明显高于模拟胃液，这表明微胶囊体系可以很好地控制红曲色素在模拟胃肠液中的释放。

关键词: 红曲色素, 复合凝聚法, 微胶囊, 稳定性

Abstract: In order to improve the stability of Monascus pigment, gelatin and gum arabic were used as wall materials, supplemented with transglutaminase (TGase) and hydroxypropyl-β-cyclodextrin, and Monascus pigment microcapsules were prepared by composite coagulation. The process conditions for composite agglomeration were determined by zeta potential, turbidity determination: the optimal ratio of gelatin to gum arabic was 1:1, and the optimal pH for the reaction was 4.0. On this basis, the microcapsule preparation process was optimized by the response surface method. Taking the embedding rate as the response value, the optimal preparation process conditions were optimized as follows: wall material concentration of 1.5% (w/v), core-to-wall ratio of 2.89, and coalescence time of 42min, under which the encapsulation rate of microcapsule Monascus pigment was 85.06%. The solubility, storage stability, photostability and in vitro release activity of the microcapsules were characterized. The results showed that the microcapsule significantly improved the storage stability of Monascus pigment and was the most stable under low temperature and room temperature conditions. By simulating the photodegradation process of Monascus pigment and its microcapsules, it was found that Monascus red pigment and Monascus orange pigment conformed to the first-order kinetic model, yellow pigment conformed to the zero-level kinetic model, and microcapsules could significantly improve the photostability of Monascus pigment. In addition, the results of in vitro release experiments showed that the microcapsule system had a good effect on delaying the release of Monascus pigment in simulated gastric juice, and the release rate in simulated intestinal fluid was significantly higher than that of simulated gastric juice, which indicated that the microcapsule system could well control the release of monascus pigment in simulated gastrointestinal fluid.

Key words: Monascus pigments, Complex coacervation, Microcapsules, Stability

中图分类号:

TS201

陈师昀宋畅陈艺玲侯思文梁梓华李文龙吕旭聪倪莉. 基于复合凝聚法制备红曲色素微胶囊及其稳定性表征[J]. 食品科学.

师昀 Lv xucong Ni Li. Preparation of Monascus Pigment Microcapsules Based on Composite Coagulation Method and Characterization of their Stability[J]. FOOD SCIENCE.

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