Determination of Sterigmatocystin in Wheat by HPLC

doi:10.7506/spkx1002-6630-201112038

Abstract

Abstract: A HPLC method has been developed to determine sterigmatocystin in wheat. The residue of sterigmatocystin in wheat samples was extracted into n-hexane and cleaned up on silica-gel SPE column for removing fat and other impurities before injection into HPLC system connected with Nova-Pak C18 column. A mobile phase composed of methanol and water (70:30, V/V) was used at a flow rate of 0.7 mL/min. The detection was performed at 246 nm. There was a good linear relationship in the range from 0.08 to 4.00μg/mL (R2 = 0.9996). The average recovery was 86.59% with a RSD of 4.70%. The limit of detection was 0.017μg/kg. This method can provide a simple, rapid and accurate method for determining sterigmatocystin in wheat and other grains.

Key words: grains, sterigmatocystin, high performance liquid chromatography (HPLC)

CLC Number:

TS212.7

YUAN Jian，DU Juan，WANG Hai-feng，JU Xing-rong. Determination of Sterigmatocystin in Wheat by HPLC[J]. FOOD SCIENCE, 2011, 32(12): 174-177.

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Determination of Sterigmatocystin in Wheat by HPLC

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