Abstract: In this work, a cyclodextrin and peptide ligand-assisted noncompetitive detection model was developed using chlorpyrifos (CPF) as typical target. The possibility of CPF encapsulated by β-cyclodextrin (β-CD) was first confirmed through the use of molecular modeling and UV and fluorescence spectroscopy. Then, bovine serum albumin (BSA)-β-CD conjugate, which was still able to include CPF, was synthesized and five positive peptide ligands specifically bound to the CPF:β-CD inclusion complexes rather other β-CD were screened from a commercialized phage-display liner dodecapeptide library. At last, a noncompetitive detection model was developed using BSA-β-CD conjugate and the most active peptide (Clone 8). The developed detection model could specially detect CPF, but not profenofos, dichlofenthion, or bromophos without the need for the modification and immobilization of the target small molecules, thereby providing an alternative method for the rapid detection of small molecules.

Key words: β-cyclodextrin, peptide, chlorpyrifos, phage display