in vitro Binding of Bile Salts by Water-soluble Proteins from Tartary Buckwheat Grains

Abstract

Abstract: Water-soluble proteins were extracted from tartary buckwheat grains by salting out with ammonium sulfate and purified by DEAE-Sepharose Fast Flow chromatography. Optimal conditions for the salting out and DEAE-Sepharose Fast Flow chromatographic separation of water-soluble proteins were determined. The results of experiments on bile salt adsorption in vitro demonstrated that the obtained water-soluble proteins had hypolipidemic effect. Besides, the best results of the elution of water-soluble proteins from DEAE-Sepharose Fast Flow column were achieved using pH 6.5, 10 mmol/L phosphate buffer as eluent at a flow rate of 1.0 mL/min and room temperature. Peak 3 was shown to have the best bile salt adsorption ability among three separation peaks obtained.

Key words: buckwheat water-soluble protein, ammonium sulfate salting-out, DEAE-Sepharose Fast Flow chromatography, in vitro bile salt adsorption, lowing blood plasma

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in vitro Binding of Bile Salts by Water-soluble Proteins from Tartary Buckwheat Grains

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