Protective effect of yak casein antioxidant peptide on oxidative stress injury in HEK293 cells

Abstract

Abstract: In this study, HEK-293 cells were induced by H2O2 to establish a oxidative damage cell model, and the optimal treatment concentration of H2O2 and time were determined. The effects of five antioxidant peptides (AFK, IEQI, FPFF, LPVPQ and RELEEL) from yak casein protein on the survival rate, malondialdehyde content, antioxidant enzyme activity level, reduced glutathione and oxidized glutathione content of HEK293 cells damaged by H2O2 were studied, and the antioxidant mechanism of antioxidant peptides from yak casein protein was discussed to provide theoretical basis for its development and application in high value-added biological products and functional foods. The results showed that HEK293 cells were treated with 400 μmol/L H2O2 for 12h, and the inhibition rate was 46.21±0.40%. Cytotoxicity test showed that five antioxidant peptides had no toxic or side effects on HEK-293 cells, nor did they promote proliferation. The antioxidant peptides can significantly reduce the content of malondialdehyde and oxidized glutathione in HEK-293 cells damaged by oxidation, and enhance the activity of antioxidant enzymes. Among them, antioxidant peptide RELEEL can significantly reduce the content of malondialdehyde (0.062±0.0004) U/104 cell, enhance the activity of glutathione (61.17±2.479) μg/106cell and maintain GSH/GSSG (64.93±0.95). The antioxidant peptide LPVPQ can significantly reduce the content of oxidized glutathione (0.74±0.26) μg/106cell l and increase the activity of superoxide dismutase (1.17±0.016) U/104 cell. Antioxidant peptide AFK can significantly enhance catalase activity (0.60±0.094) U/104 cell. These results show that antioxidant peptides from yak casein have positive effects on cells damaged by oxidation, which can provide reference for the development of related products in the next step.

Key words: yak casein, antioxidant peptides, HEK293 cells, oxidative damage, protective effect

CLC Number:

TS201.2

登宇吴. Protective effect of yak casein antioxidant peptide on oxidative stress injury in HEK293 cells[J]. FOOD SCIENCE, 0, (): 0-0.

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