FOOD SCIENCE ›› 0, Vol. ›› Issue (): 0-0.

• Marine Functional Food •     Next Articles

Protective effect of marine-derived functional peptides on oxidative stress damage in Caco-2 cells

1, 1,Xu Chen 1, 3, 1   

  • Received:2022-11-13 Revised:2023-03-03 Online:2023-04-15 Published:2023-04-19

Abstract: The purpose of this study was to investigate the effect of crimson snapper scale peptides (CSSPs) on H2O2-induced oxidative damage in Caco-2 cells, and to lay a foundation for its application in functional foods. The levels of LDH and IL-8 in the cell supernatant, the activity of intracellular antioxidant enzymes, the release of intracellular ROS and MDA, and the expression of related apoptosis genes was measured. The antioxidant effects of CSSPs on intestinal cells were elucidated from the aspects of cellular redox state and apoptosis. Results showed that CSSPs mitigated H2O2 stimulation through the antioxidant defense system, mainly including increasing the activities of antioxidant enzymes to inhibit LDH release, intracellular ROS accumulation, MDA and IL-8 production. Furthermore, CSSPs inhibited apoptosis of oxidative damaged cells by regulating the mitochondrial apoptotic pathway, which significantly increased mitochondrial membrane potential, down-regulated the mRNA expression of Caspase-3, Caspase-9 and Bax, up-regulated the mRNA expression of Bcl-2. In conclusion, CSSPs can effectively reduce the degree of cell damage by maintaining the oxidation-reduction balance and inhibiting apoptosis.

Key words: Marine-derived peptides, antioxidant, Caco-2 cells, oxidative stress damage, cell apoptosis

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