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    Antioxidant and Antibacterial Activities of Enzymatic Hydrolysate of Laminaria japonica Aresch and Its Fermented Products by Pediococcus pentosaceus
    ZHANG Li-jiao, ZENG Yan, ZHANG Huan, ZHANG Ying, MEN Yan, SUN Yuan-xia
    FOOD SCIENCE    DOI: 10.7506/spkx1002-6630-201421032
    Accepted: 06 November 2014

    WANG Yu-Zhi, Wang-Hong-Yin, LIU Yong-Zhen, GAO Gui-Lan
    FOOD SCIENCE    1982, 3 (3): 6-7.  
    Abstract10178)   HTML4)    PDF(pc) (692KB)(395)       Save
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    Study on Determination of Reducing Sugar Content Using 3,5-Dinitrosalicylic Acid Method
    ZHAO Kai, XU Peng-Ju, GU Guang-Ye
    FOOD SCIENCE    2008, 29 (8): 534-536.  
    Abstract7916)   HTML29)    PDF(pc) (486KB)(5765)       Save
    Two kinds of 3,5-dinitrosalicylic acid(DNS) agents were respectively used for determination of reducing sugar content,and effects on determination results by the factors,such as amount of DNS reagent developing time,wavelength and storage time were discussed. The results showed that on the conditions of reagent amount around 1.5 ml,boiling bath time not less than 5 minutes,and standing for 30 minutes after developing,at the wavelength of 540 nm and 550 nm for reagent one and two respectively,the accurate results can be acquired.
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    Effect of Coating on Quality Change of Chilled Pork during Storage
    XING Xin-tao,LEI Tian-tian,SUN Jing-xin,FENG Ting,LIN Ping
    FOOD SCIENCE    DOI: 10.7506/spkx1002-6630-201322060
    Accepted: 05 December 2013

    Toxicity and Estrogen Effects of Methyl Paraben on Drosophila melanodaster
    GU Wei,XIE De-juan,HOU Xiao-wei
    FOOD SCIENCE    2009, 30 (1): 252-254.   DOI: 10.7506/spkx1002-6630-200901060
    Abstract6992)   HTML8)    PDF(pc) (299KB)(1015)       Save

    The effects of methyl paraben (MP) on the fecundity and growth and development of Drosophila melanogaster were studied in this experiment. After methyl paraben was administered to Drosophila melanogaster at different concentrations, the egg number produced by parental and filial generation, the period of development, the number of pupa and eclosion and the bodyweight were analyzed. The results showed that 0.2 percent of methyl paraben has obvious toxicity effects on Drosophila melanogaster but lower concentration of methyl paraben has potential estrogenic activities. 0.2 percent of methyl paraben decreases the number of egg, pupa and eclosion, the bodyweight of male and delays the period of development. 0.02 percent of methyl paraben increases the number of egg, pupa and eclosion and shortens the period of development.

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    The Application of HACCP in the Production of Yogurt
    QIAO Shu-Qing, ZHANG Jian-Jun, LIU Zhi-Guang
    FOOD SCIENCE    2004, 25 (11): 452-456.  
    Abstract6719)   HTML8)    PDF(pc) (43KB)(880)       Save
    The developmental direction of food is safe、healthy、high quality in 21 century. HACCP system is an effectiveand rational approach to the assurance of food safety. Based on the research on the application of HACCP in the production ofyogurt in this paper. The content of hazard analysis was analyzed, and critical control points were determined and a runningpattern of HACCP was formulated. The program of corresponding control values and monitor system was determined, so as topromote the management of production quality and ensure the safety of the products.
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    Colorimetric Determination of GABA in GAD Activity Assay
    YANG Sheng-Yuan, Lu-Zhao-Xin, 吕Feng-Xia , Bie-Xiao-Mei, SUN Li-Jun
    FOOD SCIENCE    2006, 27 (7): 205-209.  
    Abstract6420)   HTML12)    PDF(pc) (52KB)(1036)       Save
    A colorimetric method of determining gama-aminobutyric acid (GABA) in glutamate decarboxylase (GAD) activity assay with high sensitivity, accuracy and reproducibility is proposed, and the standard procedure leading to the establishment of the method is described. The reaction mixture (0.4ml) of GAD and L-Glu is mixed with 1mol/L Na2CO3 (0.1ml), 0.2mol/L borate buffer (0.5ml) and 6% phenol (1.0ml), then added 1ml of 5.2% sodium hypochlorite solution at 20℃ within 5min. After being kept at 20℃ for 4~8min, the mixture is heated by boiling water bath for 10min and promptly cooled in ice bath for 20min, then added 2ml of 60% ethanol, and kept at 20℃ for 20~40min.The optical density of the mixture is measured by the absorbance at 640nm. The accuracy and sensitivity of the determination method are also discussed, indicating that the relative error is 4. 91% in comparison with HPLC method.
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    Extraction of Quercetin from Onion
    LI Bing, WU Jian-Wen, LI Lin, CHEN Ling, LI Xiao-Xi
    FOOD SCIENCE    2005, 26 (4): 137-141.  
    Abstract6222)   HTML0)    PDF(pc) (267KB)(720)       Save
    Quercetin as a natural food antioxidant was extracted from onion. By the orthogonal experiment, the optimal extraction condition was determined by NaOH solution concentration 0.03mol/L, extraction temperature 60℃, ratio of onion to solvent 1:2, extraction time 120min and the end point of acid sedimentation pH 2. The antioxidant activity was examined by the experiment of inhibiting oxidation of linoleic acid. It showed that quercetin extracted from onion exhibited good antioxidant ability.
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    Rapid HPLC Determination of Lambda-Cyhalothrin Residue in Foods
    ZHENG Ling-ling,MOU Hai-jin*,JIANG Xiao-lu
    FOOD SCIENCE    2009, 30 (12): 222-225.   DOI: 10.7506/spkx1002-6630-200912050
    Abstract6051)   HTML32)    PDF(pc) (349KB)(1010)       Save

    A HPLC method was developed for the analysis of lambda-cyhalothrin residue in drinking water (from polluted rivers) and Brassica chinensis leaves. The analysis was performed on a SB-C18 column using acetonitrile-water (80:20, V/V) as a mobile phase at the flow rate of 1.25 ml/min and detection wavelength was set as 230 nm. Compared to other solvents, acetone-n-hexane (1:1, V/V) mixture exhibited the highest extraction efficiency to lambda-cyhalothrin (the highest recovery) from drinking water. Acetone-petroleum ether (1:1, V/V) mixture led to the highest recovery for lambda-cyhalothrin from ground Brassica chinensis leaves, and vortex-mixing extraction was adopted due to the highest the highest recovery for lambda-cyhalothrin as well as its time-saving contribution. The recoveries for lambda-cyhalothrin in drinking water and ground Brassica chinensis leaves spiked at three levels were in the rages of 97.93%-103.02% and 91.4%-113.9%, respectively, and the RSDs were less than 4%. This method is proved to be easily performed and time saving (within 30 min) and can be applied in the food safety detection.

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    Simultaneous Determination by HPLC-ELSD of Ten Kinds of Sugars and Sugar Alcohols in Foods
    LIN Hui,YAN Chun-rong,XU Chun-xiang,CAI Jing,WANG Duo-jiao
    FOOD SCIENCE    DOI: 10.7506/spkx1002-6630-201312059
    Accepted: 17 June 2013

    Formation Pathways and Detection Methods of Chlorine Propanol Esters in Edible Vegetable Oil
    LIU Jing1,2,WANG Ying-yao1,*,DUAN Zhang-qun1,ZHANG Hui2,WANG Li2,WEI Cui-ping1,LUAN Xia1
    FOOD SCIENCE    2013, 34 (21): 375-378.   DOI: 10.7506/spkx1002-6630-201321074
    Abstract5914)   HTML0)    PDF(pc) (1631KB)(1316)       Save

    Chlorine propanol esters become a new potential risk factor of wide concern in edible oils.
    3-Chloropropane-1,2-diol (3-MCPD) ester is the most important material in the chlorine propanol compounds. The
    formation mechanism, control technique, detection methods and the applications of 3-chlorine propanol esters in
    edible oils system were reviewed in this paper, which will provide the understanding and control for the hazards of
    contaminant formation in the processing of edible oils.

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    HPLC Determination of Lactoferrin in Dairy Products
    XIA Ming1,2,YING Tie-jin1,*
    FOOD SCIENCE    2010, 31 (2): 165-167.   DOI: 10.7506/spkx1002-6300-201002042
    Abstract5655)   HTML13)    PDF(pc) (251KB)(1295)       Save

    A HPLC method was developed for the determination of lactoferrin in dairy products. Lactoferrin was easier to precipitate by the introduction of additional ferrous ion and 60% ethanol solution was added to precipitate proteins for removing carbohydrates. The precipitate, whose main composition was lactoferrin, was extracted with acetic acid buffer to remove casein impurity prior to HPLC analysis. The chromatographic separation was achieved on a LiChrosorb RP-C18 column using single 0.1 g/100 mL TFA as mobile phase A and a mixture of 98% methanol and 0.1 g/100 mL TFA as mobile phase B and linear gradient elution mode was used. This method exhibited a good linearity over the concentration range of 0.4 to 2 mg/mL for lactoferrin in dairy products (R2 = 0.9980), and the average recovery for lactoferrin at five spike levels was 95.4%.

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    Quantitative Determination of Tea Saponin in Tea Seed Meal by High Performance Liquid Chromatography
    ZHANG Hai-long,ZHANG Wei-nong,HU Zhi-xiong,QI Yu-tang,GONG Ren
    FOOD SCIENCE    2013, 34 (8): 153-156.   DOI: 10.7506/spkx1002-6630-201308031
    Abstract5650)   HTML6)    PDF(pc) (2258KB)(1106)       Save
    In this work, tea saponin was purified by reversed-phase ODS-A column chromatography from degreased coldpressed tea seed meal for use as a reference standard in the quantitative determination of tea saponin by HPLC and identified by UV absorption spectrometry, FTIR and mass spectrometry. HPLC separation was achieved with reversed-phase ODS column and acetonitrile-water as eluent (an initial acetonitrile concentration of 20% held for 5 min, then increased to 100% in 5 min, and finally decreased back to the initial value) at a flow rate of 1.0 mL/min in gradient mode. UV detection was carried out at 215 nm. Under these conditions, symmetric peaks, desired retention time and good separation between tea saponin and impurity were observed.
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    Nutrient Analysis of Blackberry Leaves
    FOOD SCIENCE    2012, 33 (22): 235-237.  
    Abstract5327)   HTML0)    PDF(pc) (194KB)(1733)       Save
    The major nutrients, amino acid composition and mineral element contents of blackberry leaves were analyzed and compared with those of the leaves of other plants such as wolfberry, persimmon, mulberry, Ampelopsis grossedentata and Vaccinium dunalianum. Blackberry leaves were found to have higher contents of crude protein (20.39 g/100 g), cellulose (14.52 g/100 g) and total flavonoids (3.75 g/100 g). It contained a full range of amino acids and were higher in the contents of total amino acid and essential amino acids than the leaves of other five plants. Moreover, micro-mineral elements essential for human body such as Ca, K, Mg, Na, P, Fe, Zn, Cu, Mn showed higher levels in blackberry leaves, small amounts of Cd, Cr, and Co were also found, and the Pb content was 3.18 mg/kg, which was lower than the national limit for tea. This study demonstrates that blackberry leaves can be processed into high-quality tea and hold promise for further research and development due to abundance of flavonoids.
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    Comparison of Three Detectors Used for HPLC Determination of Monoglycerides
    FANG Min,ZHAO Mou-ming,ZHAO Qiang-zhong
    FOOD SCIENCE    2013, 34 (8): 161-163.   DOI: 10.7506/spkx1002-6630-201308033
    Abstract5164)   HTML3)    PDF(pc) (2058KB)(1078)       Save
    High performance liquid chromatography-ultraviolet detector(HPLC-UV), high performance liquid chromatography-differential refractive index detector(HPLC-RI) and high performance liquid chromatography- evaporative light scattering detector(HPLC-ELSD) were compared to measure the content of monoglycerides in emulsions.When using UV detector, the result was seriously influenced by impurities, the signal of the samples was weak and the sensitivity was low. RI detector could had a good linear relationship only in the higher concentration range, so it was not suitable for measuring low content of monoglycerides. When using ELSD detector, the linear range of monoglycerides were between 0.040~5.000 mg/mL. The correlation coefficient of ELSD was 0.9998 , the recovery rate of monoglycerides reached 92.94%, the standard deviations was 2.24 and the variation coefficients was 2.41%. HPLC-ELSD method was sensitive, accurate and reproducible and may therefore be applicable for the determination of monoglycerides in emulsions .
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    Determination of Sialic Acid in Edible Birds Nest Using Pre-column Derivatization Reversed Phase High Performance Liquid Chromatography with Photodiode Array or Fluorescence Detection
    FENG Ting-yu,XUE Chang-hu,SUN Tong,CUI Hong-bo,XU Jie*
    FOOD SCIENCE    2010, 31 (8): 233-236.   DOI: 10.7506/spkx1002-6300-201008053
    Abstract5129)   HTML15)    PDF(pc) (347KB)(2073)       Save

    A pre-column derivatization RP-HPLC method with detection with a photodiode array detector (DAD) or a fluorescecse detector (FLD) was developed for the determination of N-acetylneuraminic acid in edible birds’ nest (EBN). Samples were dissolved in 0.5 mol/L sodium bisulfate aqueous solution and kept for 30 min in 80 ℃ water bath. After cooling the derivatization was carried out using O-phenylenediamine · 2HCl as derivative. The chromatographic separation was achieved on a C18 column using a mobile phase composed of 1.0% tetrahydrofuran aqueous solution (containing phosphoric acid and 1- butylamine at the levels of 0.5% and 0.15%, respectively) and acetonitrile (95:5, V/V) at a flow rate of 1.0 mL/min in the isocratic elution mode. The DAD wavelength was set at 230 nm and the FLD Ex and Em wavelengths at 230 and 425 nm, respectively. The relationship between HPLC peak area and N-acetylneuraminic acid concentration over the range of 0.1-750 μg/mL exhibited good linearity (r > 0.9990). A complete separation of N-acetylneuraminic acid was observed within 20 min. Mean recoveries of 6 replicates for N-acetylneuraminicacid in a real sample spiked at 3 levels ranged from 85.03% to 97.14%. The limit of detection (LOD) of the method was 0.2μg /mL with a DAD and 0.005μg /mL with a FLD. Clearly, the LOD with a FLD was two orders of magnitude lower than that with a DAD. Meanwhile, less miscellaneous small peaks were observed in the FLD detection. However, a DAD presented higher detection sensitivity than a FLD at the same concentrations over the LOD resulting from DAD detection. The method demonstrates the advantages of high sensitivity, good repeatability and rapidity, and is consequently most suitable for the determination of N-acetylneuraminic acid in some samples like EBN.

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    Enzymatic Extraction of Collagen from Egg Shell Membrane
    FOOD SCIENCE    2012, 33 (16): 96-99.  
    Abstract5122)   HTML0)    PDF(pc) (201KB)(905)       Save
    In order to establish an enzymatic method for collagen extraction from egg shell membrane, the influence of enzyme type, pH, enzyme dose, material-to-water ratio and extraction time on extraction efficiency was investigated and these extraction parameters were optimized one-factor-at-a-time experiments combined with an L9(34) orthogonal array design. The optimal extraction condition was hydrolysis for 5 h with 6% papain at normal temperature and a material-to-water ratio of 1:100 (g/mL), resulting in an extraction rate of 0.91%. As a result, an efficient extraction method was obtained. Collagen extraction from egg shell membrane by this method not only avoids environmental pollution caused by abandoned egg shell membrane, but also has great practical value in the collagen production industry.
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    Study on Pectin Extraction from Banana Peel and Preparation of Pectin Fresh-keeping Film
    QIU Li-ping1,JIANG Lu2,YAO Yu-jing1,CHEN Qiong1,WEN Qi-biao2
    FOOD SCIENCE    2009, 30 (8): 106-110.   DOI: 10.7506/spkx1002-6630-200908020
    Abstract5041)   HTML4)    PDF(pc) (592KB)(705)       Save

    Pectin was extracted from banana peel by four different methods, and their extraction efficiencies were compared. The pectin structure was analyzed with Fourier transform infrared spectrometer. Furthermore, the effects of pectin concentration and modified starch on the properties of edible pectin film were investigated, the preparation conditions of the edible pectin film were optimized through single-factor and orthogonal test, and scanning electronic microscope was used to observe the cross-section structure and analyze the tensile strength (TS) of the film. The results showed that all the products are pectin extracted from banana peel by four different methods. Under the same extraction temperature, time and substrate concentration, the extraction efficient of the hydrochloric acid hydrolysis and salting-out method is the highest with an extraction rate of 1.311%. In the extraction efficient of ethanol precipitation method, sulfurous acid is better than hydrochloric acid as extraction agent. When the pectin concentration is more than 1%, TS and elongation at break (EB) both raise with the increase of the pectin concentration. Also, TS and EB are improved following with the increase of thickness of films and the amount of modified starch. The main sequence of the factors affecting TS of pectin film is as follows: glycerol concentration > pectin concentration > crossed-starch concentration. Especially, the structure and TS of films prepared by pectin, glycerol and crossed-starch are the best, and the optimum concentrations of pectin, glycerol and crossed-starch are 2.4%, 0.5% and 1.0%, respectively.

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    Determination of Fructose,Glucose,Sucrose and Maltose in Honey by Liquid Chromatography Refractive Index Detector
    ZHANG Shu-Fen, SHI Ping-Ping, WANG Quan-Lin, SHEN Jian, FU Xiao
    FOOD SCIENCE    2008, 29 (6): 280-283.  
    Abstract4981)   HTML3)    PDF(pc) (216KB)(1142)       Save
    This study discussed the method for determination of fructose, glucose, sucrose and maltose content in honey by reverse-phase high performance liquid chromatogram refractive index detector (HPLC-RID). Results indicated that not only the carbohydrate can be extracted from the honey effectively, but also the mobile phase relative coherence can be guaranteed by dissolving sample (2.5 g) with distilled water (30 ml) and then diluting to 50 ml with acetonitrile. Soluble mechanism was also presumed. The excellent stability and repeatability are obtained by the developed pretreatment method. The spiked recoveris is in the range of 97%~103% with relative standard deviation of 0.8%~1.6%. Eight different honey samples were detected and good results are obtained.
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    Isolation, Purification and Antioxidant Activity of Sesame Peptide from Solid-state Fermentation
    PENG Hui-hui1,LI Lü-mu1,2,3,*,QIAN Kun3,XU Fa-zhi2,WU Dong3,ZHOU Fen3,DING Xiao-ling2
    FOOD SCIENCE    2013, 34 (9): 66-69.   DOI: 10.7506/spkx1002-6630-201309015
    Abstract4923)   HTML1)    PDF(pc) (1775KB)(924)       Save

    The antioxidant activity of small peptide from sesame meal subjected to solid-state fermentation was studied.
    Active small peptides were extracted from fermented sesame seed meal and three peptides with different molecular weights
    were separated and purified by Sephadex G-15, named as Peptides 3, 4 and 6. The antioxidant activity of sesame peptide was
    also systematically analyzed. DPPH scavenging activity was measured and salicylic acid method for the scavenging activity
    and ferric ion reducing ability method for the determination of its total reduction capacity. DPPH radical scavenging activity
    of 1 mg/mL crude extract and the peptide with 3 or 4 amino acid residues was 90%, but the DPPH radical scavenging
    activity of the peptide with 6 amino acid residues was 80%. In addition, the hydroxyl radical scavenging rate of 0.6 mg/mL
    peptide with 3 or 4 amino acid residues was 100%, while the hydroxyl radical scavenging rate of the crude extract and the
    peptide with 6 amino acid residues was 90%. Total reducing capacity of 4 mg/mL crude extract and the peptide with 4 or 6
    amino acid residues, and 2 mg/mL peptide with 3 amino acid residues was equivalent to 0.5 mg/mL glutathione. These results
    showed that small peptides from solid-state fermentation of sesame meal have strong reducing power and strong scavenging effect on
    DPPH and hydroxyl free radicals. The antioxidant activity can be enhanced when the molecular weight of sesame peptide is reduced.

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