食品科学

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慕萨莱思酿酒酵母产果胶酶及β-葡萄糖苷酶定性分析

薛菊兰1,2,程玉来1,张佰清1,朱丽霞2,*   

  1. 1.沈阳农业大学食品学院,辽宁 沈阳 110866;2.塔里木大学生命科学学院,新疆 阿拉尔 843300
  • 出版日期:2013-10-15 发布日期:2013-09-27
  • 通讯作者: 朱丽霞
  • 基金资助:

    国家自然科学基金项目(31060223);新疆兵团塔里木盆地生物资源保护利用实验室开放课题(BR0907)

Qualitative Analysis of Pectinase and β-Glucosidase Secreted by Saccharomyces cerevisiae Strains Isolated from Musalais

XUE Ju-lan 1,2,CHENG Yu-lai1,ZHANG Bai-qing1,ZHU Li-xia2,*   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;
    2. College of Life Science, Tarim University, Alaer 843300, China
  • Online:2013-10-15 Published:2013-09-27
  • Contact: ZHU Li-xia

摘要:

利用定性培养基对慕萨莱思酿酒酵母产果胶酶和β-葡萄糖苷酶活性进行测定。结果显示:分离自不同厂家(或作坊)的436株慕萨莱思酿酒酵母普遍具有果胶酶和β-葡萄糖苷酶的活性,且主要分布于较弱和中等两个等级。21株慕萨莱思酿酒酵母果胶酶活性明显高于5株商用酵母,其主要来自于现代化厂家,10株β-葡萄糖苷酶高产菌主要集中于当地作坊,说明酿造工艺与环境对野生酵母胞外酶的释放具有一定影响。

关键词: 慕萨莱思;酿酒酵母;果胶酶;&beta, -葡萄糖苷酶

Abstract:

Polygalacturonic acid agar medium and Esculin glycerol agar (EGA) medium were used to qualitatively analyze
the pectinase and β-glucosidase secreted by Saccharomyces cerevisiae strains isolated from Musalais. Results showed that
436 wild strains isolated from different manufacturers (or traditional workshops) demonstrated a low or medium ability
to secret pectinase and β-glucosidase. Compared to 5 commercial wine yeast strains, 21 wild strains from modernized
manufacturers were able to produce pectinase with higher activity while 10 strains with high β-glucosidas activity were
mainly from the local workshops. Our results revealed that brewing process and environment influenced the release of
extracellular enzymes from wild yeast.

Key words: Musalais;Saccharomyces cerevisiae;pectinase;&beta, -glucosidase

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