食品科学

• 生物工程 • 上一篇    下一篇

氯氰菊酯降解菌的分离鉴定及其降解底物谱研究

唐 洁,曾朝懿,张 庆,史 颖   

  1. 西华大学生物工程学院,四川 成都 610039
  • 出版日期:2014-06-15 发布日期:2014-07-03

Isolation, Identification, Degradation Characteristics of a Bacterial Strain Capable of Degrading Cypermethrin

TANG Jie, ZENG Chao-yi, ZHANG Qing, SHI Ying   

  1. School of Bioengineering, Xihua University, Chengdu 610039, China
  • Online:2014-06-15 Published:2014-07-03

摘要:

采用富集培养法,从长期受氯氰菊酯农药污染的果园土壤中,分离筛选得到1 株可降解氯氰菊酯的菌株N-02。经形态、生理生化特征及16S rDNA序列分析,初步鉴定为点状气单胞菌(Aeromonas punctata)。菌株N-02在35 ℃、pH 7.5、氯氰菊酯质量浓度为20 mg/L的LB培养基中培养96 h,对氯氰菊酯的降解率达到62.31%。该菌株N-02对拟除虫菊酯的降解谱较宽,培养96 h对20 mg/L高效氯氰菊酯、溴氰菊酯和氟氯氰菊酯降解率分别达到40.17%、33.26%和30.45%。

关键词: 氯氰菊酯, 点状气单胞菌, 生物降解, 降解底物谱

Abstract:

A bacterial strain named N-02 capable of degrading cypermethrin was isolated from long-term cypermethrin
contaminated orchard soil by using enrichment culture. Based on the morphology, physio-biochemical characteristics, and
16S rDNA sequence analysis, strain N-02 was identified as Aeromonas punctata. The degradation rate of cypermethrin was
62.31% when the strain were cultured at 35 ℃ for 96 h in LB medium containing 20 mg/L cypermethrin at pH 7.5. A wide
degradation spectrum of pyrethroid by strain N-02 was observed, and the degradation rates of β-cypermethrin, deltamethrin
and fluoride cypermethrin after 96 h were 40.17%, 33.26% and 30.45%, respectively.

Key words: cypermethrin, Aeromonas punctata, degradation characteristics, degradation spectrum