食品科学 ›› 2018, Vol. 39 ›› Issue (6): 116-121.doi: 10.7506/spkx1002-6630-201806019

• 生物工程 • 上一篇    下一篇

剁辣椒发酵过程中菌群与有机酸变化规律分析

叶陵1,王晶晶1,王蓉蓉1,李勇2,刘成国1,蒋立文1,邓放明1,周辉1,*   

  1. (1.湖南农业大学食品科学技术学院,湖南省食品科学与生物技术重点实验室,湖南?长沙 410128;2.张家界灵洁绿色食品有限公司,湖南?张家界 427000)
  • 出版日期:2018-03-25 发布日期:2018-03-14
  • 基金资助:
    国家自然科学基金面上项目(31571811);国家自然科学基金青年科学基金项目(31601525); 湖南省教育厅科学研究项目(16K043)

Changes in Microflora and Organic Acid Contents during the Fermentation of Chopped Pepper

YE Ling1, WANG Jingjing1, WANG Rongrong1, LI Yong2, LIU Chengguo1, JIANG Liwen1, DENG Fangming1, ZHOU Hui1,*   

  1. (1. Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China;2. Zhangjiajie Lingjie Green Food Co. Ltd., Zhangjiajie 427000, China)
  • Online:2018-03-25 Published:2018-03-14

摘要: 以自然发酵为对照,以植物乳杆菌W-4进行剁辣椒纯种发酵。研究结果表明:在发酵过程中,接种发酵能使乳酸菌快速成为优势菌,较快地降低剁辣椒的pH值。接种发酵具有较高数量的菌落总数和乳酸菌,酵母菌在自然发酵和接种发酵中数量相当,变化趋势基本一致,而大肠菌群只在发酵前期出现,而后消失。有机酸测定结果表明接种发酵具有较高含量的乳酸,而自然发酵具有较高含量的柠檬酸和苹果酸,而乙酸只在自然发酵的前期出现。

关键词: 植物乳杆菌W-4, 剁辣椒, 菌群, 有机酸

Abstract: Lactobacillus plantarum W-4 was used as a starter culture for the fermentation of chopped pepper and spontaneous fermentation was also performed as a control. The results obtained indicated that lactic acid bacteria dominated the fermentation process, and the pH of chopped pepper inoculated with L. plantarum W-4 was lower than that of spontaneous fermentation. The chopped pepper fermented with L. plantarum W-4 had higher numbers of lactic acid bacteria and colony count than spontaneous fermentation. The count and changing trend of yeast in the spontaneous fermentation were similar to those of inoculated fermentation, and?coliform?bacteria were present only in the early stage of fermentation but died at the later stage. The analysis of organic acids indicated that the chopped pepper fermented with the starter culture maintained higher content of lactic acid, while spontaneous fermentation had higher content of citric acid and malic?acid; acetic acid was present only in the early stage of spontaneous fermentation.

Key words: Lactobacillus plantarum W-4, chopped pepper, microflora, organic acids

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