食品科学 ›› 2010, Vol. 31 ›› Issue (19): 292-294.doi: 10.7506/spkx1002-6630-201019063

• 生物工程 • 上一篇    下一篇

促进酵母菌乙醇发酵新方法

惠丰立,褚学英,柯 涛,张彩莹   

  1. 南阳师范学院生命科学与技术学院
  • 收稿日期:2010-03-02 修回日期:2010-09-19 出版日期:2010-10-15 发布日期:2010-12-29
  • 通讯作者: 惠丰立 E-mail:huifl@126.com
  • 基金资助:

    河南省科技厅科技攻关项目(092102110120);河南省教育厅自然科学基金项目(2009A180012)

A Novel Method for Enhancing Ethanol Fermentation by Saccharomyces cerevisiae

HUI Feng-li,CHU Xue-ying,KE Tao,ZHANG Cai-ying   

  1. College of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China
  • Received:2010-03-02 Revised:2010-09-19 Online:2010-10-15 Published:2010-12-29
  • Contact: HUI Feng-li E-mail:huifl@126.com

摘要:

在发酵培养基中添加固态基质,研究不同固态基质对酵母菌乙醇发酵的促进作用。结果表明:在培养基中分别添加2g/100mL 的麦秸杆、花生壳、稻壳和锯末,可使发酵液中乙醇质量浓度提高了15.5%~24.8%。在4种不同固态基质中,稻壳对酵母菌乙醇发酵的促进作用最显著。以稻壳作为固态基质,确定了添加固态基质条件下酵母菌乙醇发酵的最适条件为:稻壳2.5g/100mL、初糖12g/100mL、温度30℃、发酵时间48h。在适宜的发酵条件下,发酵液中乙醇质量浓度可达到51.8g/L,为理论产率的92.7%。

关键词: 酵母菌, 乙醇发酵, 固态基质, 稻壳

Abstract:

Solid matrix was added to fermentation medium for exploring the effect of solid matrix on ethanol fermentation in yeast cells. Results indicated that the addition of 2 g of straw, peanut hull, rice hull or wood shavings to 100 mL medium could improve ethanol production by 15.5%-24.8%. Rice hull was the most suitable solid matrix and the optimal technological parameters for rice hull were found to be: rice hull amount 2.5 g/100 mL; initial glucose concentration 12 g/100 mL; fermentation temperature 30 ℃, and fermentation time 48 h. Under these optimal conditions, the actual production of alcohol reached up to 51.8 g/L, which was 92.7% of the theoretical value.

Key words: yeast, ethanol fermentation, solid matrix, rice hull

中图分类号: