关键词: 毛细管电泳, 三聚氰胺, 含量测定, 奶制品

Abstract:

A method using high performance capillary electrophoresis (HPCE) was presented for determination of melamine in dairy products. An HPCE apparatus equipped with an untreated fused-silica capillary (75 μm × 78 cm, 70 cm effective length) was used. Mobile phase was 30 mmol/L phosphate buffer at pH 5.0. UV detection wavelength was set at 240 nm and separation voltage at 10 kV. The regression curve for peak area versus melamine concentration revealed a good linearity in the range of 10.0－50.0μg/mL, with a correlation coefficient of 0.9992. The spike recovery was averaged at 97.04%, with a relative standard deviation (RSD) of 1.24% (n = 3). The method exhibits characteristics of simplicity, rapidity and good repeatability, and consequently can be used for melamine determination in dairy products.

Key words: HPCE, melamine, content determination, dairy products