食品科学 ›› 2009, Vol. 30 ›› Issue (23): 314-317.doi: 10.7506/spkx1002-6300-200923071

• 生物工程 • 上一篇    下一篇

海洋链霉菌LY-1的抗细菌特性研究

刘 姝1,房耀维1,徐炜枫2,吕明生1,王淑军1,陈 丽1   

  1. 1.淮海工学院海洋学院,江苏省海洋生物技术重点建设实验室 2.江苏省农产品质检中心
  • 收稿日期:2009-05-14 修回日期:2009-08-20 出版日期:2009-12-01 发布日期:2010-12-29
  • 通讯作者: 刘姝 E-mail:jdliushu@yahoo.com.cn
  • 基金资助:

    江苏省淮海工学院自然科学研究项目(Z2007032);江苏省淮海工学院科研启动项目(KQ07101)

Antibacterial Property of Marine Streptomlyces sp. LY-1

LIU Shu1,FANG Yao-wei1,XU Wei-feng2,LU Ming-sheng1,WANG Shu-jun1,CHEN Li1   

  1. 1. School of Marine Science and Technology, Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Insititute of Technology,
    Lianyungang 222005, China;2. Jiangsu Agro-product Quality Test Center, Nanjing 210036, China
  • Received:2009-05-14 Revised:2009-08-20 Online:2009-12-01 Published:2010-12-29
  • Contact: LIU Shu E-mail:jdliushu@yahoo.com.cn

摘要:

从连云港海域潮间带所采的土样中分离到一株产高活性抗细菌物质的链霉菌LY-1。抗菌谱测定结果表明菌株LY-1 发酵产物对枯草芽孢杆菌等5 株革兰氏阳性菌,大肠杆菌等6 株革兰氏阴性菌有显著拮抗作用。稳定性研究表明该菌所产抗细菌物质在121℃,pH1 和pH12 条件下抑菌活性均不变;紫外线照射也不影响其抑细菌活性。该抗菌产物对蜡样芽孢杆菌和大肠杆菌生长的抑制作用研究表明,该抗菌产物能够较好的抑制蜡样芽孢杆菌和大肠杆菌的生长繁殖。菌株LY-1 所产抗菌物质在海产品及食品保鲜、生防及医药方面具有潜在的应用价值。

关键词: 海洋链霉菌LY-1, 发酵产物, 抗细菌活性

Abstract:

Marine Streptomyces sp.LY-1 solated from marine samples collected in the intel tidal zone of Lianyungang, was found to produce antibacterial substance which exhibited significant inhibitory effects on 5 Gram-positive bacteria and 6 Gramnegative bacteria. The results of stability studies of the antibacterial substance showed that it had very good stability at pH1 and pH12 under 121 ℃ or under ultraviolet treatment. Sensibility and inhibition effect of antibacterial substance produced by strain LY-1 to Bacillus cereus and Escherichia coli were studied. The results showed that Bacillus cereus and Escherichia coli were very sensitive to antibacterial substance. Antibacterial substance could strongly inhibit their growth-breeding. The potential use of substance produced by strain LY-1 in bio-control, marine products, food and medical application was predicted.

Key words: Marine Streptomyces sp. LY-1, fermentation product, antibacterial activity

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