鸡红细胞Cu,Zn-SOD的纯化及部分性质研究

食品科学 ›› 2008, Vol. 29 ›› Issue (2): 266-270.

鸡红细胞Cu,Zn-SOD的纯化及部分性质研究

张兰杰, 候冬岩, 辛广, 袁勤生

鞍山师范学院化学系；华东理工大学生命科学学院辽宁鞍山114007；辽宁鞍山114007；上海200237；

出版日期:2008-02-15 发布日期:2011-08-24

Study on Purification and Properties of Superoxide Dismutase from Erythrocytes of Chicken

ZHANG Lan-Jie, HOU Dong-Yan, XIN Guang, YUAN Qin-Sheng

1.Department of Chemistry, Anshan Normal University, Anshan 114007, China;2.College of Life Science, East China University of Science Technology, Shanghai 200237, China

Online:2008-02-15 Published:2011-08-24

摘要/Abstract

摘要： 目的:从鸡红细胞分离铜锌超氧化物歧化酶(Cu,Zn-SOD),并对其部分性质进行研究。方法:用氯仿-乙醇除杂蛋白、丙酮沉淀、热变性、DEAE-32柱色谱等方法,对鸡红细胞Cu,Zn-SOD进行分离纯化。结果:得到Cu,Zn-SOD干粉5.9mg,比活10196.27U/mg蛋白,活力回收70.43%,纯化倍数为125.82。理化性质分析表明:该酶的最大紫外吸收波长264nm,相对分子量约为31000D,相对亚基分子量约为16500D,pH在6～9范围内稳定性很好,在40～60℃内保温30min酶活基本不变,2mmol/LSDS对此酶没有明显的抑制,该酶对H2O2和KCN敏感。结论:鸡红细胞含有丰富的Cu,Zn-SOD。

关键词: 鸡红细胞, 铜锌超氧化物歧化酶, 纯化, 性质

Abstract: Objective:To purify copper/zinc superoxide dismutase (Cu, Zn-SOD) from erythrocytes of chicken and to study some properties of purified Cu, Zn-SOD. Methods:Cu, Zn-SOD has been purified from erythrocytes of chicken by chloroform-ethanol fractionation, acetone precipitation, thermal denaturalization and DEAE-32 chromatography. Results:5.9 mg of dry powder enzyme was obtained, the purified specific activity of the enzyme was 10196.27 U/mg protein, the yield was 70.46%, and its purification time was 125.82. The investigation of physiochemical properties of the SOD showed that the ultraviolet absorption peak of the enzyme was found to be at 264 nm, which has two identical subunit, the molecular weight about 31000 D and the molecular weigh of subunit about 16500 D. The enzyme was found to have good stability at the pH range from 6 to 9 and good thermal stability at the 40 ℃ to 60 ℃ range over 30 mimutes incubation period. The Cu, Zn-SOD was not obviously inhibited by 2 mol/L SDS, but obviously inhibited by H2O2 and KCN. Conclusion:Cu, Zu-SOD is rich in the erythrocytes of chicken.

Key words: erythrocytes of chicken, copper and zinc superoxide dismutase, purification, property

张兰杰, 候冬岩, 辛广, 袁勤生. 鸡红细胞Cu,Zn-SOD的纯化及部分性质研究[J]. 食品科学, 2008, 29(2): 266-270.

ZHANG Lan-Jie, HOU Dong-Yan, XIN Guang, YUAN Qin-Sheng. Study on Purification and Properties of Superoxide Dismutase from Erythrocytes of Chicken[J]. FOOD SCIENCE, 2008, 29(2): 266-270.

[1]	项婷，夏琛，刘健华，王超然，沈建福. 蛹虫草中新化合物的分离纯化、结构鉴定及抗肿瘤活性[J]. 食品科学, 2021, 42(8): 235-242.
[2]	王丽波，高婧宇，李腾飞，李莲玉，刘博，张洪龑，杨昱，徐雅琴. 硒化蒲公英多糖的制备、结构表征及益生菌促增殖活性[J]. 食品科学, 2021, 42(7): 169-175.
[3]	王伟，李津津，迟海. 解淀粉芽孢杆菌素Amylocyclicin W5的纯化及其抑菌机理[J]. 食品科学, 2021, 42(7): 29-34.
[4]	翟一潭，柏玉香，李晓晓，王禹，邱立忠，卞希良，金征宇. 酶法改性淀粉颗粒的研究进展[J]. 食品科学, 2021, 42(7): 319-328.
[5]	张启月，张士凯，郗良卿，杜海云，吴澎. 不同提取方法对樱桃酒渣水溶性膳食纤维结构、理化与功能性质的影响[J]. 食品科学, 2021, 42(7): 98-105.
[6]	吴晓娟，吴伟. 米糠酸败诱导的蛋白质氧化对米糠清蛋白界面性质的影响[J]. 食品科学, 2021, 42(6): 8-15.
[7]	杨蒙，薛正莲，甘玉飞，周杰，王洲，刘艳. 辅助蛋白基因的剪接对磷脂酶A1酶活的影响[J]. 食品科学, 2021, 42(6): 104-110.
[8]	王存堂，高增明，姜辰昊，孔保华. 茶叶乙醇提取物对蜡质玉米淀粉回生性质的影响[J]. 食品科学, 2021, 42(6): 53-60.
[9]	鲁振杰，李娟，陈正行，李诚，李亚男. 麦麸阿拉伯木聚糖的羧甲基化改性及理化性质表征[J]. 食品科学, 2021, 42(6): 61-67.
[10]	周一鸣，杜丽娜，李云龙，周小理，陈智东. 高静水压和热处理对荞麦蛋白功能性质的影响[J]. 食品科学, 2021, 42(5): 77-83.
[11]	魏晨业，包晓玮，王娟，何梦梦，曾兰君，张亚涛. 沙棘多糖分离纯化及抗氧化活性[J]. 食品科学, 2021, 42(4): 227-232.
[12]	吴晓娟，王晓婵，张佳妮，沈佳丽，李依，金曼芹，吴伟. pH值碱性偏移结合热处理对米糠蛋白结构和功能性质的影响[J]. 食品科学, 2021, 42(4): 23-30.
[13]	李妍，崔维建，赵城彬，吴玉柱，王思琪，曹勇，许秀颖，刘景圣. 玉米淀粉-玉木耳多糖复配体系理化及结构性质[J]. 食品科学, 2021, 42(4): 58-64.
[14]	彭常梅，方锐琳，赖敏，邓泽元，刘小如，李静. 不同提取方法对牡丹籽油品质的影响[J]. 食品科学, 2021, 42(3): 104-111.
[15]	胡晓，周雅，陈星星，李来好，杨贤庆，陈胜军，吴燕燕，杨少玲. 罗非鱼肌浆蛋白源抗氧化肽的制备、分离纯化及其体外抗氧化活性[J]. 食品科学, 2021, 42(3): 63-70.