食品科学 ›› 2008, Vol. 29 ›› Issue (6): 460-465.

• 专题论述 • 上一篇    下一篇

ATP生物发光法在微生物检验中的应用

 唐倩倩, 叶尊忠, 王剑平, 盖铃, 应义斌, 李雁斌   

  1. 浙江大学生物系统工程与食品科学学院; 阿肯色大学 浙江杭州310029; 浙江杭州310029; 美国阿肯色州菲也特维尔72701;
  • 出版日期:2008-06-15 发布日期:2011-08-26

Application of ATP Bioluminescence in Microbial Detection

 TANG  Qian-Qian, YE  Zun-Zhong, WANG  Jian-Ping, GAI  Ling, YING  Yi-Bin, LI  Yan-Bin   

  1. 1. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China; 2. University of Arkansas, Fayetteville, Arkansas 72701, USA
  • Online:2008-06-15 Published:2011-08-26

摘要: ATP生物发光法是一种快速的微生物检测方法,具有简便、灵敏度高、可实时检测等优点。本文就ATP生物发光法的检测原理、检测步骤、特点、检测的影响因素及在食品工业中的应用进行了综述,并提出了目前ATP生物发光法检测中存在的问题。

关键词: ATP生物发光法, 荧光素-荧光素酶, 提取剂, 温度, pH

Abstract: ATP bioluminescence is one kind of rapid detection method for microorganisms, since it is easy, highly sensitive and timely, etc. In this paper, principles, procedures, characteristics, influencing factors of ATP bioluminescence and its application in food industry are introduced. Meanwhile, its drawbacks are presented. To sum up, ATP bioluminescence is potential fora pplication.

Key words: ATP bioluminescence, luciferin-luciferase, extractant, temperature, pH