食品科学 ›› 2005, Vol. 26 ›› Issue (4): 55-58.

• 基础研究 • 上一篇    下一篇

一株高产γ-亚麻酸丝状真菌菌株的发酵接种方式研究

 戴传超, 史央, 王安琪, 袁志林   

  1. 南京师范大学生命科学学院
  • 出版日期:2005-04-15 发布日期:2011-09-19

Studies on Inoculation Types of γ-linolenic Acid Fermentation by a High-producing Filamentous Fungus Strain

 DAI  Chuan-Chao, SHI  Yang, WANG  An-Qi, YUAN  Zhi-Lin   

  1. College of Biology Science, Nanjing Normal University
  • Online:2005-04-15 Published:2011-09-19

摘要: 从土壤中筛选到一株高产γ-亚麻酸(GLA)的丝状真菌,GLA占总脂肪酸的12.85%,菌丝含油量为19.70%。经鉴定为根霉属真菌。比较两种不同的接种方式发现,孢子接种发酵所得菌丝体干物质产量、GLA含量以及油脂含量分别比菌丝接种高25.98%、51.90%和43.87%。用菌丝制作液体种子二级发酵,其GLA含量可以达到孢子接种水平,但菌丝生物量仍然低于孢子接种组。

关键词: &gamma, -亚麻酸, 根霉, 孢子接种, 菌丝接种, 液体种子

Abstract:  A filamentous fungi strain identified as Rhizopus sp., producing high γ-linolenic acid (GLA) was obtained. In its mycelium, GLA amounted to 12.85% of the fatty acid while oil amounted to 19.70% of the mycelia. Two inoculation types were compared. By the spore inoculation the biomass, GLA composition and oil content of the mycelia were respectively 4.17g/L, 18.41% and 28.34%. By using mycelia inoculation gots the results were 25.98%, 51.90%, 43.87% higher than those above respectively. Using the mycelium as the secondary inoculums the final GLA content could reach the same level as that of the spores’; but the biomass could not meet the level of the spore inoculation.

Key words: γ-linolenic acid, Rhizopus sp., spore inoculation, mycelia inoculation, liquid seed