铜绿微囊藻(Microcystic aeruginosa)胞外和胞内酸性多糖分离、纯化方法的比较及产物的鉴定

食品科学 ›› 2007, Vol. 28 ›› Issue (12): 244-248.

铜绿微囊藻(Microcystic aeruginosa)胞外和胞内酸性多糖分离、纯化方法的比较及产物的鉴定

张玲, 周斌伟, 梅秋红, 王习达, 陆长梅, 吴国荣

南京师范大学生命科学院；南京师范大学生命科学院江苏南京210097；江苏南京210097；江苏南京210097深圳市北科生物科技有限公司；广东深圳518100；

出版日期:2007-12-15 发布日期:2011-11-22

Comparison of Separation and Purification Methods between Intra-and Extra-cellular Acidic Polysaccharides from Microcystic aeruginosa and Identification of Their Products

ZHANG Ling, ZHOU Bin-Wei, MEI Qiu-Hong, WANG Xi-Da, LU Chang-Mei, WU Guo-Rong

1.College of Life Science, Nanjing Normal University, Nanjing 210097, China; 2.Beike Biotechnology Co. Ltd., Shenzhen 518000, China

Online:2007-12-15 Published:2011-11-22

摘要/Abstract

摘要： 铜绿微囊藻(Microcystic aeruginosa)属于蓝藻,是水华爆发的主体藻类。本实验在不同pH值和温度条件下分别提取其胞内和胞外多糖的粗制品,采用DEAE-纤维素离子交换柱层析,分离出其中含有醛酸基团的酸性组分,再用具分子筛效应的SephadexG-150和G-100进一步纯化出具HPLC单一对称峰的两个胞外酸性多糖组分和一个胞内酸性多糖组分。分别测定和分析三种酸性多糖的分子量、糖醛酸及单糖的组成。

关键词: 铜绿微囊藻, 胞内酸性多糖, 胞外酸性多糖, 分离, 鉴定

Abstract: Microcystic aeruginosa, a kind of blue-green alga, is the main alga in algae bloom. In this study extra-and intra-cellular crude polysaccharides were purified from M.aeruginosa by different temperature and pH. Their acidic components which had alduronic acid groups were purified by DE-52, and then acidic polysaccharides of the two groups from intra-cellular and one group from extra-cellular were purified by Sephadex columns. Each of these three acidic polysaccharides presents a single HPLC peak in its respective chromatographs of DE-52 and Sephadex G-150 columns. These three acidic polysaccharides are different from each other in their molecular weights, alduronic acid contents and monosaccharides constitutents.

Key words: Microcystic aeruginosa, intracellular acidic polysaccharide (IAPS), extracellular acidic polysaccharide (EAPS), isolation, identify

张玲, 周斌伟, 梅秋红, 王习达, 陆长梅, 吴国荣. 铜绿微囊藻(Microcystic aeruginosa)胞外和胞内酸性多糖分离、纯化方法的比较及产物的鉴定[J]. 食品科学, 2007, 28(12): 244-248.

ZHANG Ling, ZHOU Bin-Wei, MEI Qiu-Hong, WANG Xi-Da, LU Chang-Mei, WU Guo-Rong. Comparison of Separation and Purification Methods between Intra-and Extra-cellular Acidic Polysaccharides from Microcystic aeruginosa and Identification of Their Products[J]. FOOD SCIENCE, 2007, 28(12): 244-248.

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