食品科学 ›› 2006, Vol. 27 ›› Issue (12): 594-596.
• 分析检测 • 上一篇 下一篇
詹玲,潘思轶
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ZHAN Ling, PAN Si-Yi
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摘要: 通过DEAE-Cellulose52离子交换层析和SephadexG-200凝胶层析从大豆中提取纯化一种糖蛋白,并对其结构进行了初步分析。用SDS-PAGE检测纯度,并测定其分子质量的大小,测定结果为61000,蛋白质含量为30.55%;β-消除反应说明其为O-连接型糖蛋白,红外光谱法推断其含有α型糖苷键。
关键词: 大豆糖蛋白, 分离纯化, 结构分析
Abstract: A soybean glycoprotein was isolated and purificated from soybean by using DEAE-Cellulose 52 IEC(Ion-exchange Chromatography) and Sephadex G-200 SEC(Size-exchange Chromatography). The soybean glycoprotein was analysed by SDS-PAGE for its purity and molecular mass.The content of the soybean glycoprotein is 30.55% and the molecular mass is 61000.β-elimination reaction indicated the linkage type of the soybean glycoprotein is O-glycosidic linkage and IR Spectra is α-glycosidel inkage.
Key words: soybean glycoprotein, isolation, purification, structure analysis
詹玲, 潘思轶. 大豆糖蛋白的分离纯化及结构分析[J]. 食品科学, 2006, 27(12): 594-596.
ZHAN Ling, PAN Si-Yi. Isolation,Purification and Analysis of Glycoprotein from Soybean[J]. FOOD SCIENCE, 2006, 27(12): 594-596.
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