虫草头孢菌粉提取物对乳鼠心肌细胞博动节律异常的影响

食品科学 ›› 2006, Vol. 27 ›› Issue (12): 745-747.

虫草头孢菌粉提取物对乳鼠心肌细胞博动节律异常的影响

邹峥嵘，王乃利，姚新生

江西师范大学生命科学学院；深圳中药及天然药物研究中心；

出版日期:2006-12-15 发布日期:2011-11-23

Antiarrhythmic Effects on Neonatal Rat Cardiac Myocytes of Extracts from the Mycelia of Cephalosporium sinensis

ZOU Zheng-rong1,2,WANG Nai-li2,YAO Xin-sheng

1.School of Life Science, Jiangxi Normal University, Nanchang 330022, China; 2.Shenzhen Research Center of Tradional Chinese Medicine and Natural Medicine, Shenzhen 518055, China

Online:2006-12-15 Published:2011-11-23

摘要/Abstract

摘要： 目的:研究虫草头孢菌粉提取物对乳鼠心肌细胞博动节律异常的影响。方法:以氯化钙和异丙肾上腺素诱发博动节律异常的心肌细胞为模型组,分别以维拉帕米和普萘洛尔作阳性对照,溶剂为阴性对照,记录给药前后心肌细胞的博动频率和节律。结果:虫草头孢菌粉乙醇提取物(Cs-E)、虫草头孢菌粉乙醇提取物的乙酸乙酯萃取物(Cs-E-Et)和虫草头孢菌粉乙醇提取物的正丁醇萃取物(Cs-E-Bu)对于分别由氯化钙、异丙肾上腺素诱发的乳鼠原代培养心肌细胞正性频率作用和节律失常均有明显的改善效应。结论:虫草头孢菌粉乙醇提取物的乙酸乙酯萃取物和正丁醇萃取物是抑制心肌细胞博动节律异常的有效部位。

关键词: 虫草头孢菌粉, 乳鼠心肌细胞, 博动节律异常

Abstract: Objective: To investigate the antiarrhythmic effects on neonatal rat cardiac myocytes of extracts from the mycelia of Cephalosporium sinensis. Methods: The model arrhythmia of neonatal rat cardiac myocytes induced by CaCl2 and and isoprenaliner espectively in vitro, the antiarrhythmic effects of verapamil, propranolol, fractions of the extracts from the mycelia of Cephalosporium sinensis and the solvent of fractions were tested respectively. Results: EtOH extracts from the mycelia of Cephalosporium sinensis. (Cs-E), the fraction of Cs-E partitioned by EtOAc(Cs-E-Et), and the fraction of Cs-E partitioned by n-BuOH(Cs-E-Bu) had the antiarrhythmic effects on neonatal rat cardiac myocytes induced by the CaCl2 and isoprenaline respectively in vitro. Conclusion: EtOH extracts from the mycelia of Cephalosporium sinensis. (Cs-E), the fraction of Cs-E partitioned by EtOAc(Cs-E-Et), and the fraction of Cs-E partitioned by n-BuOH(Cs-E-Bu) were the bioactive fractions in fermented Mycelia of Cephalosporium sinensis (Ningxinbao).

Key words: Mycelia of Cephalosporium sinensis, neonatal rat cardiac myocytes, arrhythmia

邹峥嵘，王乃利，姚新生. 虫草头孢菌粉提取物对乳鼠心肌细胞博动节律异常的影响[J]. 食品科学, 2006, 27(12): 745-747.

ZOU Zheng-rong1,2,WANG Nai-li2,YAO Xin-sheng. Antiarrhythmic Effects on Neonatal Rat Cardiac Myocytes of Extracts from the Mycelia of Cephalosporium sinensis[J]. FOOD SCIENCE, 2006, 27(12): 745-747.

[1]	邹峥嵘1，陈贝贝1，王如伟2，姚新生3,*. 虫草头孢菌粉抗心律失常活性成分的分离纯化及结构鉴定[J]. 食品科学, 2012, 33(21): 149-152.
[2]	邹峥嵘, 袁宜如, 赖海华. 虫草头孢菌粉粗多糖的理化性质和含量测定研究[J]. 食品科学, 2007, 28(9): 86-88.