关键词: 菌种改良, 重组工程技术, 基因打靶

Abstract: The technique of improving industrial microbe strain experienced three development stages: mutagenesis, genetic recombinant and genetic engineering. As the latest genetic engineering technique, gene targeting technique has the characteristics of precise positioning and stable heredity of new gene. With gene targeting technique, people can have destination to reform the hereditary material of microorganism, and create the new species microorganism in favor of the production. Recombineering is the technique which promotes homologous recombination between the targeting DNA using an independent phage recombina-tion system with a 40～60bp homologous sequence, and accurately modifies the target gene in chromosome DNA or the vectors in E.coli. By using of the recombineering technique, the length of homologous sequence of gene targeting vector can reach 10kb, even exceed 100kb, hence the efficiency of homologous recombination can increase hundreds times. The technique of recombineering has trend to replace the traditional recombinant DNA technique, providing the new and efficient means for the gene targeting technique, and would be widely used in the fields of the foundation theories research and practical application in improvement ofi ndustrial microbe strain.

Key words: train improvement, recombineering, gene targeting