食品科学 ›› 0, Vol. ›› Issue (): 0-0.
• 工艺技术 • 下一篇
祝海娟1,2,赵晨霞1,张翌楠1
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摘要: 摘 要:采用碱提法提取大麦虫蛹中的蛋白质。以蛋白质提取率为评价指标,通过单因素试验研究了碱液浓度、料液比(W/V)、提取温度、提取时间对大麦虫蛹中的蛋白质提取率的影响。在单因素试验分析的基础上,通过正交试验优化提取工艺。结果表明:大麦虫蛹中蛋白质的最佳提取工艺条件为碱液浓度1.0 g/100mL、料液比1∶10 (g/mL)、提取温度60℃、提取时间2h。在此条件下,通过三次验证试验所得蛋白质产品的粗蛋白含量为82.13%,蛋白质提取率为81.54%。
关键词: 关键词:大麦虫, 蛹, 蛋白质, 提取率, 优化
Abstract: Abstract: Protein was extracted from pupae of zophobas morio with alkali extraction method. Took the protein extraction rate as an evaluation index, the influences of aqueous alkali concentration, solid-liquid ratio(W/V), extracting temperature, extracting time on the protein extraction rate from pupae of zophobas morio by single factor test were investigated. On the basis of single factor test, optimized the extraction process using orthogonal test. The results indicated that the optimum extraction conditions of protein in zophobas morio pupae are: aqueous alkali concentration 1.0 g/100mL, solid-liquid ratio 1:10 (g/mL), extracting temperature 60℃, extracting time 2h. The protein content of the product obtained at the optimal condition by three times verification test was 82.13%, and the protein extraction rate was 81.54%.
Key words: Key words: zophobas morio, pupae, protein, extraction rate, optimization
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中图分类号:TS201.2
祝海娟 赵晨霞 张翌楠. 大麦虫蛋白质提取工艺的优化[J]. 食品科学, 0, (): 0-0.
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