分散固相萃取-HPLC检测绿茶饮料中吡虫啉和啶虫脒残留

食品科学 ›› 0, Vol. ›› Issue (): 0-0.

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分散固相萃取-HPLC检测绿茶饮料中吡虫啉和啶虫脒残留

路海燕,张媛媛,李斌,陈忠正,高雄,林晓蓉,魏超田

华南农业大学食品学院

收稿日期:2012-09-27 修回日期:2013-09-26 出版日期:2013-10-25 发布日期:2013-09-28
通讯作者: 张媛媛 E-mail:zhangyy@scau.edu.cn
基金资助:
农业部现代农业产业技术体系专项资金

Determination of Imidacloprid and Acetamiprid in Green Tea Infusion by High-performance Liquid Chromatography with Dispersive Solid Phase Extraction

Received:2012-09-27 Revised:2013-09-26 Online:2013-10-25 Published:2013-09-28
Contact: Yuan-yuan Zhang E-mail:zhangyy@scau.edu.cn

摘要/Abstract

摘要： 建立了分散固相萃取-高效液相色谱-二极管阵列检测器分析茶饮料中吡虫啉和啶虫脒残留的方法。样品经乙酸乙酯/环己烷(5/5，V/V)萃取，25mg N-丙基乙二胺(PSA)+25mg石墨化炭黑(GCB)分散固相萃取净化。采用Agilent ZORBAX-XDB C18色谱柱分离，乙腈和水洗脱。在0.05、0.10和0.50mg/L加标水平上，吡虫啉和啶虫脒的回收率分别为93.3～102.5%、95.0～112.7%，相对标准偏差(RSD)为2.2～2.9%、3.0～5.1%。吡虫啉和啶虫脒的检测限分别为0.004、0.001mg/L，定量限分别为0.010、0.006mg/L。方法精密度较高，重现性较好。

关键词: 吡虫啉, 啶虫脒, 茶饮料, 分散固相萃取, 高效液相色谱(HPLC)

Abstract: A rapid analytical method based on high-performance liquid chromatography-diode array detector was developed for the simultaneous determination of imidacloprid and acetamiprid in green tea infusion. The target analytes were extracted with ethyl acetate + cyclohexane (5/5, V/V), and the extracts were cleaned up by dispersive solid phase extraction with 25 mg primary secondary amine (PSA) +25 mg graphitized carbon black (GCB). The recoveries of three levels spiked at 0.05 mg/L, 0.1 mg/L, 0.5 mg/L were 93.3%～102.5% for imidacloprid and 95.0%～112.7% for acetamiprid, the relative standard deviations were 2.2%～2.9% and 3.0%～5.1%, respectively. The limits of detection (LOD) of imidacloprid and acetamiprid were 0.004,0.001mg/L, and the limits of quantification (LOQ) were 0.010,0.006mg/L, respectively.

Key words: Imidacloprid, Acetamiprid, Green Tea Infusion, Dispersive Solid Phase Extraction, High-performance Liquid Chromatography (HPLC)

中图分类号:

O657

路海燕张媛媛李斌陈忠正高雄林晓蓉魏超田. 分散固相萃取-HPLC检测绿茶饮料中吡虫啉和啶虫脒残留[J]. 食品科学, 0, (): 0-0.

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分散固相萃取-HPLC检测绿茶饮料中吡虫啉和啶虫脒残留

Determination of Imidacloprid and Acetamiprid in Green Tea Infusion by High-performance Liquid Chromatography with Dispersive Solid Phase Extraction

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