膜醭毕赤酵母对草莓采后灰霉病抗病性的诱导

摘要/Abstract

摘要：

研究拮抗菌膜醭毕赤酵母(Pichia membranaefaciens)对草莓采后灰霉病的控病效果，同时从诱导抗病性的角度探讨膜醭毕赤酵母对草莓灰霉病的生物防治机理。结果表明：酵母的浓度与控病效果有关，浓度越高，控病效果越好，1×107CFU/mL酵母悬浮液对草莓采后灰霉病有显著的控制效果，且不会对果实表皮造成损伤。拮抗酵母菌通过诱导草莓果实β-1,3-葡聚糖酶、过氧化物酶、苯丙氨酸解氨酶等抗性相关酶活性的增强，提高了果实对灰霉病的抵抗能力。结果说明了膜醭毕赤酵母能够用于控制草莓果实采后灰霉病，诱导果实抗病性是其作用机制之一。

关键词: 膜醭毕赤酵母, 草莓, 诱导抗病性, 灰霉病

Abstract:

Strawberry is highly susceptible to pathogen infection during storage, and gray mold caused by Botrytis cinerea
is one of the common diseases of strawberry fruit. The ability of the yeast Pichia membranaefaciens to control gray mold
of strawberry fruit during storage were examined in this study. The possible biocontrol mechanism of P. membranaefaciens
was also discussed. It was showed that there was a positive correlation between yeast concentration and the effect of disease
control. Treatment with 1 × 107 CFU/mL P. membranaefaciens suspension resulted in lower disease incidence of gray mold, and
had no harm to the epidermis. The fruit treated with P. membranaefaciens demonstrated accumulated activities of defense-related
enzymes such as β-1,3-glucanase (GLU), phenylalanine ammonia-lyase (PAL) and peroxidase (POD) in strawberry fruit. These
results indicated that P. membranaefaciens could effectively reduce the incidence of gray mold in strawberry. The induction of
resistance was one of the most important mechanisms of P. membranaefaciens control of strawberry gray mold.

Key words: Pichia membranaefaciens, strawberry, induced disease resistance, gray mold

中图分类号:

S609.3

张璐，张瑶，刘丽丹，曾凯芳. 膜醭毕赤酵母对草莓采后灰霉病抗病性的诱导[J]. 食品科学, 2013, 34(22): 286-291.

ZHANG Lu，ZHANG Yao，LIU Li-dan，ZENG Kai-fang. Induction of Disease Resistance to Gray Mold by Pichia membranaefaciens in Postharvest Strawberry Fruit[J]. FOOD SCIENCE, 2013, 34(22): 286-291.

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