辐照对有机弱酸类防腐剂抑菌性的影响

doi:10.7506/spkx1002-6630-201417012

食品科学

辐照对有机弱酸类防腐剂抑菌性的影响

李树锦，高美须，崔承弼，刘超超，牟慧，赵鑫，王志东

1.延边大学农学院，吉林延吉 133002；2.中国农业科学院农产品加工研究所，北京 100193

出版日期:2014-09-15 发布日期:2014-09-12

Effects of Irradiation on Antimicrobial Activity of Weak Organic Acid Preservatives

LI Shu-jin, GAO Mei-xu, CUI Cheng-bi, LIU Chao-chao, MOU Hui, ZHAO Xin, WANG Zhi-dong

1. College of Agriculture, Yanbian University, Yanji 133002, China; 2. Institute of Agro-products Processing Science and
Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Online:2014-09-15 Published:2014-09-12

摘要/Abstract

摘要：

为研究辐照对有机弱酸类防腐剂抑菌性的影响，实验以革兰氏阴性（G－）菌肠炎沙门氏菌、福氏志贺氏菌、大肠杆菌和革兰氏阳性（G+）菌单增李斯特菌、金黄色葡萄球菌、枯草芽孢杆菌、肠膜明串珠菌为目标菌株，采用酶标比浊法测定山梨酸钾、双乙酸钠、丙酸钠、丙酸钙和脱氢醋酸钠在固态粉末和溶液状态下，经0、5、10、15 kGy的60Co γ射线辐照后抑菌性的变化，同时确立酶标比浊法的检测波长和防腐剂质量浓度。结果表明：5 kGy以下的低剂量辐照对防腐剂的抑菌性基本不产生影响，10～15 kGy辐照对防腐剂的抑菌性产生影响，能使多数防腐剂的抑菌性增加，且辐照剂量越高影响越大；辐照对防腐剂溶液抑菌性的影响比固态防腐剂粉末大；G+菌对辐照防腐剂抑菌性的变化比G－菌更敏感。

关键词: 辐照, 防腐剂, 抑菌性影响, 酶标比浊法

Abstract:

This study was intended to investigate the effect of 60Co γ-ray irradiation on the antimicrobial activity of weak
organic acid preservatives, potassium sorbate, sodium diacetate, sodium propionate, calcium propionate and sodium
acetate dehydrogenation. Irradiation dosages were set as 0, 5, 10 and 15 kGy. The target strains were Gram-negative (G–)
bacteria including Salmonella enteritidis, Shigella flexneri, Escherichia coli, and Gram-positive (G+) bacteria including
Listeria monocytogenes, Staphylococcus aureus, Bacillus subtilis and Leuconostoc mesenteroides. Meanwhile, the detection
wavelength and suitable preservative concentrations for microtiter plate assay were established. The results showed that
low dose irradiation under 5 kGy had little effect on the antimicrobial activity of preservatives, but 10–15 kGy irradiation
could increase the antimicrobial activity of most preservatives with a positive dose-effect relationship. Irradiation made the
antimicrobial activity of preservatives more sensitive in solution than in the solid state. It was observed that the change in
antimicrobial activity of preservatives to G+ bacteria after irradiation was more sensitivity than to G – bacteria.

Key words: irradiation, preservatives, antimicrobial activity, microtiter plate assay

中图分类号:

TQ047.6

李树锦，高美须，崔承弼，刘超超，牟慧，赵鑫，王志东. 辐照对有机弱酸类防腐剂抑菌性的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201417012.

LI Shu-jin, GAO Mei-xu, CUI Cheng-bi, LIU Chao-chao, MOU Hui, ZHAO Xin, WANG Zhi-dong. Effects of Irradiation on Antimicrobial Activity of Weak Organic Acid Preservatives[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201417012.

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辐照对有机弱酸类防腐剂抑菌性的影响

Effects of Irradiation on Antimicrobial Activity of Weak Organic Acid Preservatives

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