关键词: 转基因玉米59122, 定量检测方法, 方法学评价

Abstract:

Genetically modified maize (Event 59122) used as a feedstock has been approved to be imported to China
since 2012. But, there has so far been no quantitative assay available to detect the genetically modified corn 59122 and
its derivatives. In this study we established a quantitative detection method for this modified maize corn using real-time
fluorescent quantitative PCR in order to accurately examine whether it is spread unintentionally, ensure the safety of corn
production in China and reduce the ecological risk. Meanwhile, the method was evaluated by several methodological
indicators such as specificity, sensitivity, accuracy and measurement uncertainty. Our experimental results showed that this
method was specific for modified maize corn 59122. The average of 16 replicate detections for 1% modified maize corn
59122 was 0.9% with a relative deviation of 10%. The recovery was 90%, and the measurement uncertainty was 0.002. The
lowest detectable level was 2 copies of DNA fragments from the modified maize crop 59122. The variation coefficient for
the remaining 13 replications was 0.05 when the 3 replicate detections deviated largely from the average value (0.9%) were
removed. Therefore, the quantitative detection method described in this paper for modified maize maize 59122 has fairly
high specificity, accuracy, sensitivity and precision as well as low measurement uncertainty.

Key words: genetically modified maize 59122, quantitative detection method, methodological assessment