桑叶中α-葡萄糖苷酶抑制剂产生菌的分离鉴定及诱变选育

摘要/Abstract

摘要： 从桑叶中筛选出一株糖尿病潜在治疗药物（α-葡萄糖苷酶抑制剂）产生菌，为进一步提高抑制剂量，采用常压室温等离子体（atmospheric and room temperature plasma，ARTP）技术进行了诱变育种，并初步研究其理化性质及稳定性。在分离得到的188株桑叶内生菌中，以4-硝基苯-α-D-呋喃葡糖苷（4-Nitroph-enylα-D-glucopyranoside，PNPG）法筛选α-葡萄糖苷酶抑制剂，筛选到一株细菌XuW-LB-188，其发酵上清液对α-葡萄糖苷酶抑制率达52.67%。根据菌株形态特性及16S rDNA序列，初步鉴定为萎缩芽孢杆菌。对此菌株进行ARTP诱变，高通量筛选880株突变株，其中突变株T-690抑制活性较出发菌株提高了40.61%，抑制率高达73.25%。实验结果表明，该α-葡萄糖苷酶抑制剂主要存在于发酵液中，是一种极性较大的水溶性胞外产物，且具有良好的热稳定性。

关键词: α-葡萄糖苷酶抑制剂, 桑叶, 内生菌, 筛选, 常压室温等离子体（ARTP）诱变, 萎缩芽孢杆菌

Abstract: A strain capable of producing α-glucosidase inhibitor from mulberry leaf was screened. Subsequently, atmospheric and room temperature plasma (ARTP) mutation breeding technique was taken to raise the production of inhibitors. At the same time, the physicochemical properties and stability were investigated preliminarily. Among the 188 isolated strains, a bacteria named XuW-LB-188 was selected using PNPG as substrate to screen α-glucosidase inhibitors, with inhibition ratio of 52.67% in the fermentation centrifugal supernatant. On the basis of morphological characters and 16S rDNA gene sequence, the strain was preliminarily identified as Bacillus atrophaeus. Combining ARTP mutation breeding with high-throughput screening, a mutant strain named T-690 was obtained from 880 mutant strains, whose inhibition ratio (73.25%) was increased by 40.61% compared with the original strain. The experiment results indicated that the α-glucosidase inhibitor mainly existed in broth. The inhibitor was an extracellular product with high polarity and good thermostability.

Key words: α-glucosidase inhibitor, mulberry leaf, endophytes, screening, atmospheric and room temperature plasma(ARTP), Bacillus atrophaeus

中图分类号:

Q939.97

朱孟峰许伟邵荣韦萍. 桑叶中α-葡萄糖苷酶抑制剂产生菌的分离鉴定及诱变选育[J]. 食品科学, 0, (): 0-0.

XU Wei. Isolation, Identification and Mutation Breeding of Strain Producing α-Glucosidase Inhibitor from Mulberry Leaf[J]. FOOD SCIENCE, 0, (): 0-0.

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