EGCG对米饭中污染物镉的肠吸收转运影响研究

摘要/Abstract

摘要： 近年随着水体和土壤污染的加剧，我国大米镉污染的形势较为严峻。人体的镉暴露途径主要是膳食，消化后主要在机体内的小肠部位吸收。本研究首先测定大米及蒸煮米饭中污染物镉的含量，利用已建立的体外口腔-胃-肠消化模型来测定米饭消化后镉的生物可给性，最后采用Caco-2/HT-29细胞共培养肠吸收模型来研究表没食子儿茶素没食子酸酯（(-)-epigallocatechin-3-gallate, EGCG）干预对米饭中镉吸收转运率的影响。结果表明：胃消化2 h的生物可给性为73.58±1.92%，肠消化7 h的生物可给性为36.29±1.25%；EGCG干预后米饭消化液中镉的吸收转运率显著降低，与对照组相比，21.82 μmol/L和43.64 μmol/L EGCG使镉的吸收转运率分别降低了5.56%和13.89%，该研究为镉暴露地区和人群的膳食策略提供了新的思路。

关键词: 镉（Cd）, 米饭, 表没食子儿茶素没食子酸酯（EGCG）, Caco-2/HT-29细胞共培养, 吸收转运率

Abstract: In recent years, with the intensification of water and soil pollution, the situation of Chinese cadmium pollution in rice is getting more serious. The main way for cadmium exposure is through diet, and then it is absorbed in the small intestine of the body. In this study, the cadmium content that contaminated in rice and cooked rice were firstly analyzed, and then the bioaccessibility of cadmium after digestion was determined by the established in vitro oral-gastric-intestinal digestion model. Finally, Caco-2/HT-29 cells co-culture model was used to study the effect of (-)-epigallocatechin-3-gallate (EGCG) on absorption and transport rate of cadmium in digestive fluid of cooked rice. The results indicated that the bioaccessibility of the gastric phase was 73.58±1.92% after simulated digestion for 2 h, and the intestinal stage was 36.29±1.25% after simulated digestion for 7 h. Moreover, EGCG significantly reduced the absorption and transport rate of cadmium in digestive fluid of cooked rice, and 21.82 μmol/L and 43.64 μmol/L EGCG reduced the absorption and transport rate of cadmium by 5.56% and 13.89% compared with the control group, respectively. The study may provide a new idea in dietary intervention for cadmium exposure areas and populations.

Key words: cadmium（Cd）, cooked rice, (-)-epigallocatechin-3-gallate (EGCG), Caco-2/HT-29 cells Co-culture, absorption and transport rate

中图分类号:

TS213.3

何嫱吕倩吴跃林亲录贾红玲宁亚丽. EGCG对米饭中污染物镉的肠吸收转运影响研究[J]. 食品科学, 0, (): 0-0.

Qian LV. Effects of EGCG on Intestinal Absorption and Transport of Pollutant-Cadmium in Cooked Rice[J]. FOOD SCIENCE, 0, (): 0-0.

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