应用PCR-DGGE研究玉米储藏过程中的霉菌变化

摘要/Abstract

摘要： 玉米是我国最主要的储粮作物之一，但在玉米储存过程中容易发生霉菌污染问题，造成严重的经济损失。研究储粮中霉菌污染的发生规律，构建储粮中微生物生长预测模型，从而采取相应措施减少霉菌及真菌毒素的污染，是提高我国粮食安全和食品安全的重要途径。本文利用变性梯度凝胶电泳（Denaturing Gradient Gel Electrophoresis，DGGE）研究了粮库中不同贮存时间和不同空间位置的玉米样品中霉菌群落的特点，结果表明，本研究所采样的储粮玉米中霉菌污染以青霉、曲霉和毛霉为主，储粮玉米中霉菌群落的变化与其贮存时间具有较强的相关性，而与其在粮库中的空间位置相关性较小。在贮存时间方面，贮存1年和贮存3年的储粮玉米样品中霉菌群落具有较大的差异，而贮存2年样品的霉菌群落处于过渡期状态。本文初步探究的储粮玉米中霉菌群落的时空分布特征，可以为建立准确可靠霉菌群落模型提供理论支持和数据支持。

关键词: 玉米, 霉菌, 变性梯度凝胶电泳（DGGE）

Abstract: Corn is one of the most important stored grains in China. However, in the process of storage, corn is very common to be contaminated by molds, which could cause serious economic losses. It is an important way to improve food security and food safety in our country by studying the occurrence of molds contamination and constructing prediction model of molds in stored grain to guide the prevention of mold contamination. In this article, the Denaturing Gradient Gel Electrophoresis (DGGE) was used to research the molds community composition in stored grain corn of different years and different spatial location in the grain depot. The results revealed that Penicillium, Aspergillus and Mucor were the main molds found in the stored grain corn. The years of storage were closely related to the molds community composition in stored grain corn, while the spatial location in the grain depot showed much less relationship to it. The corn samples stored for 1 year and 3 years showed great difference in their molds community composition, while the corn samples stored for 2 years were in a period of transition. The results of this study could provide important theory and data for construction of molds prediction model in stored grain.

Key words: Corn, Molds, Denaturing Gradient Gel Electrophoresis (DGGE)

中图分类号:

农业科学

岳晓禹张华陈威风邹建李欣杨娜. 应用PCR-DGGE研究玉米储藏过程中的霉菌变化[J]. 食品科学, 0, (): 0-0.

参考文献

[1] EATON D L, JOHN D. The toxicology of aflatoxins: human health, veterinary, and agricultural significance[M]. Elsevier, 2013.
[2] SORRENTI V, DI GIACOMO C, ACQUAVIVA R, et al. Toxicity of ochratoxin A and its modulation by antioxidants: A review[J]. Toxins, 2013, 5(10): 1742-1766. DOI: 10.3390/toxins5101742
[3] 孙武长, 刘桂华, 杨红, 等. 粮食中真菌及真菌毒素污染调查[J]. 中国公共卫生, 2005, 21(12): 1532-1532.
[4] 周玉庭, 任佳丽, 张紫莺. 粮食中霉菌污染检测方法现状及发展趋势[J]. 食品安全质量检测学报, 2016 (1): 244-250.
[5] 沈飞, 吴启芳, 刘兵, 等. 粮食真菌毒素污染的无损检测方法研究进展[J]. 食品安全质量检测学报, 2014 (8): 2372-2377.
[6] 成博伦, 赵玉濛, 胡锦涛, 等. 几种常见真菌毒素脱毒方法的研究进展[J]. 基因组学与应用生物学, 2015, 34(11): 2338-2344. DOI: 10.13417/j.gab.034.002338
[7] 王长才, 葛万军, 冼子君, 等. 真菌毒素生物脱毒机理的研究进展[J]. 饲料与畜牧: 新饲料, 2011 (8): 41-43.
[8] 岳晓禹, 张恒业, 辛婷, 等. 储粮预测微生物模型的研究进展[J]. 中国粮油学报, 2012, 27(5): 118-123.
[9] 钟雪美, 王建有. 不同储粮方式的霉菌动态变化及空间格局研究[J]. 陕西农业科学, 1994 (4): 7-9.
[10] 李听听, 陈伟, 李广富, 等. 不同储藏条件下玉米中霉菌对黄曲霉毒素 B1的影响[J]. 食品与发酵工业, 2014, 40(6): 211-215. DOI: 10.13995/j.cnki.11-1802/ts.2014.06.035
[11] 吴红萍, 翟世博, 杜晨辉, 等. 海南省储粮稻谷的霉菌多样性分析[J]. 贵州农业科学, 2015, 43(4): 138-141.
[12] 燕平梅, 乔宏萍, 赵文婧, 等. 基于 PCR-DGGE 方法分析榨菜中乳酸菌群落结构[J]. 食品科学, 2016, 37(13): 136-139. DOI: 10.7506/spkx1002-6630-201613024
[13] 李秀秀, 曾维伟, 陆兆新, 等. 鲫鱼贮藏过程中微生物菌相 PCR-DGGE 分析及其防腐保鲜[J]. 食品科学, 2017, 38(5): 274-280. DOI: 10.7506/spkx1002-6630-201705045
[14] 李听听, 陈伟, 李广富, 等. 玉米储藏霉菌类群及玉米赤霉烯酮含量的研究[J]. 中国粮油学报, 2015, 30(12): 92-97.
[15] Melo S C, Pungartnik C, Cascardo J C, et al. Rapid and efficient protocol for DNA extraction and molecular identification of the basidiomycete Crinipellis perniciosa[J]. Genet. Mol. Res, 2006, 5(4): 851-855.
[16] Tapia-Tussell R, Lappe P, Ulloa M, et al. A rapid and simple method for DNA extraction from yeasts and fungi isolated from Agave fourcroydes[J]. Molecular Biotechnology, 2006, 33(1): 67-70.
[17] 吴发红, 黄东益, 黄小龙, 等. 几种真菌DNA提取方法的比较[J]. 中国农学通报, 2009, 25(8): 62-64.
[18] 李听听. 玉米和小麦储藏中真菌多样性及真菌毒素的研究[D]. 山东农业大学, 2015:
[19] HAMMER ?, HARPER D A T, RYAN P D. PAST: Paleontological Statistics Software Package for Education and Data Analysis Palaeontol. Electronica 4: 1–9[J]. 2001.
[20] SEGATA N, IZARD J, WALDRON L, et al. Metagenomic biomarker discovery and explanation[J]. Genome biology, 2011, 12(6): R60. DOI: 10.1186/gb-2011-12-6-r60
[21] 王若宇, 杜丽平, 刘非, 等. PCR-DGGE 分析西藏雪莲菌细菌多样性[J]. 现代食品科技, 2017, 33(2): 83-88. DOI: 10.13982/j.mfst.1673-9078.2017.2.013
[22] 邹欣涛, 万幸, 张韧, 等. 基于 PCR-DGGE 技术分析白木香结香前后内生真菌的变化[J]. 中药新药与临床药理, 2015, 2: 023. DOI：10.19378/j.issn.1003-9783.2017.03.024
[23] 柳尧波, 傅建功, 任素芳, 等. 应用PCR-DGGE分析发酵床养殖仔猪消化道菌群多样性及其演替[J]. 家畜生态学报, 2017, 38(3): 14-18.
[24] TIAN L, CHEN S, LIU H, et al. In Vivo Effects of Pichia Pastoris-Expressed Antimicrobial Peptide Hepcidin on the Community Composition and Metabolism Gut Microbiota of Rats[J]. PloS one, 2016, 11(10): e0164771. DOI: 10.1371/journal.pone.0164771
[25] GUO M, BAO Q, CHEN S, et al. Effects of neutrophils peptide-1 transgenic Chlorella ellipsoidea on the gut microbiota of male Sprague–Dawley rats, as revealed by high-throughput 16S rRNA sequencing[J]. World Journal of Microbiology and Biotechnology, 2016, 32(3): 1-7. DOI: 10.1007/s11274-015-1994-z