酶法提取对玉米皮AX组成及分子量分布的影响

摘要/Abstract

摘要： 摘要：采用玉米皮为原料，利用酶法提取玉米皮阿拉伯木聚糖（AX），对所得到的玉米皮阿拉伯木聚糖提取物，分别进行气相色谱分析和HPSEC分析，研究Grindmyl Powerbake 950、Pentopan mono BG、Viscozyme L、Amano HC 90四种食品工业中常见的木聚糖酶提取得到的玉米皮阿拉伯木聚糖的单糖组成和分子量分布的变化，从而筛选出提取玉米皮AX的最佳酶制剂。结果表明：采用酶法提取玉米皮阿拉伯木聚糖时，其单糖组成主要是阿拉伯糖和木糖。以Amano HC 90提取的玉米皮阿拉伯木聚糖，其纯度为70.51%，提取率为17.37 ± 2.74%，重均分子量为67.87 ± 1.46×104，数均分子量为15.06 ± 0.09×104，相对分子质量分散系数为4.51 ± 0.13。经Amano HC 90提取得到的阿拉伯木聚糖纯度高，提取率适当，相对分子质量高且分散均匀，更适合食品工业中鲜玉米皮中的阿拉伯木聚糖的提取。

关键词: 玉米皮, 酶解, 单糖组成, 分子量分布

Abstract: Abstract: Arabinoxylans are extracted from maize barn by enzymatic extraction. The obtained maize barn arabinan xylan extract was subjected to gas chromatographic analysis and HPSEC analysis. And, the composition and molecular weight distribution of monosaccharides were studied under different enzyme treatments.Screening of suitable enzymes for preparation of maize barn arabinoxylans from four common xylanases in the food industry. The results showed that the composition of monosaccharides was mainly arabinose and xylose.Extracted from Amano HC 90 ways of maize barn arabinoxylan, Purity was 70.51%, the extraction rate was 17.37 ± 2.74%, the weight average molecular weight was 67.87 ± 1.46×104, the number average molecular weight was 15.06 ± 0.09×104, the relative molecular mass dispersibility was 4.51 ± 0.13. Use Amano HC 90 extracts obtained arabinoxylan high purity, the extraction rate is appropriate, and high molecular weight dispersed homogeneously, more suitable for the food industry extraction of fresh corn bran arabinoxylans.

Key words: Maize bran, Hydrolysis, Monosaccharide composition, Molecular mass distribution

中图分类号:

TS209

谷春梅尹佳玉姜雷. 酶法提取对玉米皮AX组成及分子量分布的影响[J]. 食品科学, 0, (): 0-0.

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