普洱茶对膳食诱导肥胖大鼠的降低体质量及调节细胞因子的作用

摘要/Abstract

摘要： 本研究以正常体重的雄性SD（Sprague-Dawley）大鼠作为正常对照组，将膳食诱导的肥胖SD大鼠随机分为5组（每组8只）：肥胖对照组，西布曲明阳性药物对照组和低、中、高浓度的普洱茶处理组，每天灌胃给药，每周测体重，连续给药42天后禁食24 h，眼底取血，测定血清中的葡萄糖，胰岛素，瘦素，脂联素，肿瘤坏死因子-α（TNF-α），血管内皮生长因子（VEGF），白细胞介素1（IL1）α，IL1β和低密度胆固醇（LDL-C）、高密度胆固醇（HDL-C）的含量。结果表明，低、中、高浓度普洱茶处理组与肥胖对照组相比，大鼠体重分别显著下降了24.80％，26.59％和27.62％(P＜0.05)； LDL-C下降7.11%，9.11%和25.74%（P＜0.05），葡萄糖下降57.3%，67.1%，216.7%（P＜0.01），胰岛素下降8.6%，17.2%，41.7%，瘦素下降68.58%，66.81%，64.60%，VEGF下降25.0%，27.8%，33.3%（P <0.01）和TNF-α下降45.3％，44.6%，40.5%（P <0.01）； HDL-C升高20.4％，22.2％，25.0％（P＜0.05）；脂联素浓度显着增加5.9％，7.6％和9.7％（P <0.05），调节IL-1α/IL-1β的比例，因此，普洱茶对高脂饮食诱导的肥胖大鼠具有减轻体重和调节细胞因子的作用。

关键词: 普洱茶, 饮食诱导肥胖, 细胞因子, 体质量

Abstract: Normal weight SD rats were used as (i) normal control group, and Obese SD rats were randomly divided into five groups (8 in each group): (ii) obese model group, (iii) sibutramine positive control group, (iv) three Pu-erh tea groups with low-, middle-, and high-level. The rats were given Pu-erh tea by gavage，and the body weights of the rats were measured once a week. The glucose (Glu), insulin, leptin, adiponectin, tumor necrosis factor-alpha (TNF-α), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), interleukin-1alpha/ beta (IL-1α/β) and vascular endothelial growth factor (VEGF) levels were determined in the serum when each group of rats fasting 24 h were sacrificial after continuous administration over 42 days to observe the influence of Pu-erh tea on weight loss and cytokine profiles. In contrast to the obese control, the weight of the rats decreased by 24.8%, 26.59% and 27.62% (P＜0.05), respectively. The levels of three Pu-erh tea’s groups were obviously decreased respectively of Glu 57.3%, 67.1% and 216.7%（P＜0.01）, LDL-C 7.11%，9.11% and 25.74% (P＜0.05), insulin 8.6%，17.2% and41.7%, leptin 68.58%, 66.81% and 64.60%, VEGF 25.0%, 27.8% and 33.3% (P <0.01), and TNF-α 45.3％，44.6%，40.5% (P <0.01). The concentrations of HDL-C were also significantly increased by 20.4％，22.2％，25.0％（P＜0.05）, adiponectin by 5.9％，7.6％和9.7％（P <0.05）. The ratio of IL-1α and IL-1β were modulated. Therefore, Pu-erh tea obviously reduces weight gain and the levels of LDL-C, Glu, insulin, leptin VEGF and TNF-α, while increasing the concentrations of HDL-C and adiponectin. The levels of anti-inflammatory cytokines were elevated, while the inflammatory cytokines were decreased.

Key words: Pu-erh tea, nutritional adiposity, cytokines, weight mass

中图分类号:

R151.4

王雪青. 普洱茶对膳食诱导肥胖大鼠的降低体质量及调节细胞因子的作用[J]. 食品科学, 0, (): 0-0.

Xue-Qing WANG. Pu-erh tea’s weight loss and cytokine modulation profiles of nutritionally obese Sprague-Dawley rats[J]. FOOD SCIENCE, 0, (): 0-0.

参考文献

[1]苏静静，王雪青，宋文军，等.普洱茶茶色素对小鼠的预防肥胖作用[J].食品工业科技, 2014, 35(19):346-350
[2] SANO M, TAKENAKA Y, KOJIMA R, et al.Effects of Pu-erh tea on lipid metabolism in rats[J]. Chemical & Pharmaceutical Bulletin, 1986, 34:221-228.[J].Chemical & Pharmaceutical Bulletin, 1986, 34(1):221-228
[3] QI Y, HOU I C, FUJITA H, YAZAWA K, et al.Antiobesity effects of Chinese black tea (pu-erh tea) extract and gallic acid[J]. Phytotherapy Research, 2012, 26:475-481. DOI: 10.1002/ptr.3602[J].Phytotherapy Research, 2012, 26(4):475-481
[4]TANG W, LI S, LIU Y, et al.Anti-diabetic activity of chemically profiled green tea and black tea extracts in a type 2 diabetes mice model via different mechanisms[J].Journal of Functional Foods, 2013, 5(4):1784-1793
[5]苏静静，王雪青，宋文军, 等.普洱茶对小鼠血糖的干预作用[J].食品科学, 2014, 35(9):260-263
[6]CAO Z H, GU D H, LIN Q, et al.Effect of Pu-erh tea on body fat and lipid profiles in rats with diet-induced obesity[J].Phytotherapy Research, 2011, 25(2):234-238
[7] 陈亚蓝，王雪青, 王怡雯, 等.普洱茶茶色素对HepG2细胞脂质代谢的影响及作用机理[J]. 2017, 38(17):203-209.DOI: 10.7506/spkx1002-6630-201717033[J].食品科学, 2017, 38(17):203-209
[8]DEKA A, VITA J A.Tea and cardiovascular disease[J].Pharmacological Research, 2011, 64(2):136-145
[9] 希雨.常饮黑茶可以保护心脏健康[J]. 国外医药(植物药分册), 2000, (05):228-229.[J].国外医药(植物药分册), 2000, 15(5):228-229
[10] YANG C S, WANG H, SHERIDAN Z P.Studies on prevention of obesity, metabolic syndrome, diabetes, cardiovascular diseases and cancer by tea[J]. Journal of Food and Drug Analysis, 2018, 26:1-13.DOI: 10.1016/j.jfda.2017.10.010[J].Journal of Food and Drug Analysis, 2018, 26(1):1-13
[11]ZHAO L J, JIA S T, TANG W R, et al.Pu-erh tea inhibits tumor cell growth by down-regulating mutant p53[J].International Journal of Molecular Sciences, 2011, 12(11):7581-7793
[12] LEE L K, FOO K Y.Recent advances on the beneficial use and health implications of Pu-Erh tea[J]. Food Research International, 2013, 53: 619-628. DOI:10.1016/j.foodres.2013.02.036[J].Food Research International, 2013, 53(2):619-628
[13]JUGE-AUBRY C E, HUENRICHOT E, MEIER C A.Adipose tissue: a regulator of inflammation[J].Best Practice & Research Clinical Endocrinology & Metabolism, 2005, 19(4):547-566
[14]LANTHER N, LECLERCQ I A.Adipose tissues as endocrine target organs[J].Best Practice & Research Clinical Gastroenterology, 2014, 28(4):545-558
[15] STOLARCZYK E.Adipose tissue inflammation in obesity: a metabolic or immune response?[J]. Current Opinion in Pharmacology, 2017, 37:35-40. DOI: 10.1016/j.coph.2017.08.006[J].Current Opinion in Pharmacology, 2017, 37(12):35-40
[16]KUBOTA K, SUMI S, TOJO H, et al.Improvements of mean body mass index and body weight in preobese and overweight Japanese adults with black Chinese tea (Pu-Erh) water extract[J].Nutrition Research, 2011, 31(6):421-428
[17]EMANUELE D A, PEI G, LISA P et al.Lipid-related markers and cardiovascular disease prediction[J].Jama the Journal of the American Medical Association, 2012, 307(23):2499-2506
[18]SUPERKO H R, PENDYALA L, WILLIAMS P T, et al.High-density lipoprotein subclasses and their relationship to cardiovascular disease[J].Journal of Clinical Lipidology, 2012, 6(6):496-523
[19]JAGANATHAN R, RAVINDRAN R, DHANASEKARAN S.Emerging role of adipocytokines in type 2 diabetes as mediators of insulin resistance and cardiovascular disease. Canadian Journal of Diabetes, 2017. DOI:10.1016/j.jcjd.2017.10.040[J].Canadian Journal of Diabetes, 2017, :1-12
[20]ZWICK R K, GUERRERO-JUAREZ C F, HORSLEY V, et al.Anatomical,physiological,and functional diversity of adipose tissue[J].Cell Metabolism, 2018, 27(1):68-83
[21]WALSH J M, MCGOWAN C A, BYRNE J A, et al.The association between TNF-α and insulin resistance in euglycemic women[J].Cytokine, 2013, 64(1):208-212
[22]PORTER M H, CUTCHINS A, FINE J B et al.Effects of TNF-α on glucose metabolism and lipolysis in adipose tissue and isolated fat-cell preparations[J].Journal of Laboratory & Clinical Medicine, 2002, 139(3):140-145
[23] NAIKOO N A, DIL-AFROZE, RASOOL R, et al.Upregulation of vascular endothelial growth factor (VEGF), its role in progression and prognosis of non-small cell lung carcinoma[J]. Cancer Genetics, 2017, 216:67-73. DOI: 10.1016/j.cancergen.2017.07.005[J].Cancer Genetics, 2017, 216-217:67-73
[24] ABDEL-RAHMAN O.Targeting vascular endothelial growth factor (VEGF) pathway in gastric cancer: Preclinical and clinical aspects[J]. Critical Reviews in Oncology/hematology. 2015, 93(1):18-27. DOI: 10.1016/j.critrevonc.2014.05.012[J].Critical Reviews in Oncology/hematology, 2015, 93(1):18-27
[25]LOUIE S M, ROBERTS L S, NOMURA D K.Mechanisms linking obesity and cancer[J].Biochimica et Biophysica Acta, 2013, 1831(10):1499-1508
[26]PALOMO J, DIETRICH D, MARTIN P, et al.The interleukin (IL)-1 cytokine family–balance between agonists and antagonists in inflammatory diseases[J].Cytokine, 2015, 76(1):25-37