文蛤寡肽对高脂饮食诱导小鼠肾损伤的修护作用

doi:10.7506/spkx1002-6630-201815022

食品科学 ›› 2018, Vol. 39 ›› Issue (15): 145-151.doi: 10.7506/spkx1002-6630-201815022

文蛤寡肽对高脂饮食诱导小鼠肾损伤的修护作用

王佳佳，叶蕾，杨最素*，余方苗，丁国芳

浙江海洋大学食品与医药学院，浙江省海洋生物医用制品工程技术研究中心，浙江舟山 316022

出版日期:2018-08-15 发布日期:2018-08-15
基金资助:
浙江省自然科学基金项目（LY15C200016；LQ16H300001）；舟山市级公益类科技项目（2015C31012）

Protective Effect of Meretrix meretrix Oligopeptides on Kidney Injury Induced by High Fat Diet in Mice

WANG Jiajia, YE Lei, YANG Zuisu*, YU Fangmiao, DING Guofang

Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics of Zhejiang Ocean University, Zhoushan 316022, China

Online:2018-08-15 Published:2018-08-15

摘要/Abstract

摘要： 目的：研究文蛤寡肽（Meretrix meretrix oligopeptides，MMO）对高脂饮食诱导小鼠肾损伤的修护作用。方法：建立高脂饲料致小鼠慢性肾损伤模型，通过MMO灌胃30 d后，测定小鼠肾脏指数，检测小鼠血清甘油三酯（triglyceride，TG）、总胆固醇（total cholesterol，TC）、高密度脂蛋白胆固醇（high density lipoprotein cholesterol，HDL-C）、低密度脂蛋白胆固醇（low density lipoprotein cholesterol，LDL-C）浓度，以及小鼠肾组织匀浆中超氧化物歧化酶（superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力和丙二醛（malondialdehyde，MDA）含量，计算小鼠动脉粥样硬化指数（atherosclerosis index，AI），苏木素-伊红（hematoxylin-eosin，HE）染色观察小鼠肾脏病理学改变，免疫组化法测定肾组织α-平滑肌肌动蛋白（α-smooth muscle actin，α-SMA）、转录生长因子-β1（transforming growth factor-β1，TGF-β1）蛋白表达量。结果：MMO组与模型组比较，小鼠肾脏指数，血清中TG、TC、LDL-C浓度及AI显著下降，HDL-C浓度显著升高，肾组织匀浆中SOD、GSH-Px活力显著升高，MDA含量显著降低，HE染色观察肾组织结构明显好转，免疫组化结果显示MMO可降低肾损伤小鼠α-SMA、TGF-β1蛋白表达水平。结论：MMO对高脂饮食诱导的小鼠肾损伤有较为明显的修护作用。

关键词: 文蛤寡肽, 高脂饲料, 肾损伤, 修护作用

Abstract: The protective effect of Meretrix meretrix oligopeptide (MMO) on high fat diet-induced kidney injury in mice was examined. A chronic kidney injury model was established by feeding a high fat diet to mice, and then the mice were lavaged with MMO for 30 days. Kidney index was measured, and serum triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) contents were determined as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) levels in kidney homogenate. In addition, atherosclerosis index (AI) was calculated and renal pathological changes were examined by hematoxylin-eosin (HE) staining. Immunohistochemical staining was used to detect the protein expression of α-SMA and TGF-β1 in kidney tissue. The results showed that treatment with MMO significantly decreased kidney index, serum TG, TC and LDL-C levels and AI and increased serum HDL-C level as compared to the model group. Treatment with MMO significantly elevated SOD and GSH-Px activities and lowered MDA level in liver homogenate. The immunohistochemical assay showed that MMO markedly down-regulated the protein expression level of α-SMA and TGF-β1 in kidney tissue. Therefore, MMO can effectively protects against chronic kidney injury induced by high fat diet in mice.

Key words: Meretrix meretrix oligopeptides, high fat diet, kidney injury, protective effect

中图分类号:

TS201.6

王佳佳，叶蕾，杨最素，余方苗，丁国芳. 文蛤寡肽对高脂饮食诱导小鼠肾损伤的修护作用[J]. 食品科学, 2018, 39(15): 145-151.

WANG Jiajia, YE Lei, YANG Zuisu, YU Fangmiao, DING Guofang. Protective Effect of Meretrix meretrix Oligopeptides on Kidney Injury Induced by High Fat Diet in Mice[J]. FOOD SCIENCE, 2018, 39(15): 145-151.

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文蛤寡肽对高脂饮食诱导小鼠肾损伤的修护作用

Protective Effect of Meretrix meretrix Oligopeptides on Kidney Injury Induced by High Fat Diet in Mice

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