关键词: 番茄，SINAC4，代谢组学，代谢产物

Abstract: tomato fruit has important economic and nutritional value. According to previous studies, transcription factors play a key role in gene expression in plants, however, its function on tomato fruit metabolites is still unclear. In this study, the SINAC4 gene silencing tomato fruit and wild type tomato fruit were used to investigate metabolites of tomato fruit by metabonomics in breaking stage by using high performance liquid chromatography (HPLC) and high resolution mass spectrometer (MS) to study the effect of transcription factor SINAC4 on metabolites at the start-up of tomato fruit ripening. The compounds were qualitatively analyzed by using the XCMS software and the database of inhouse standard, HMDB, KEGG, and so on. The quantitative analysis and differential metabolite screening were carried out by metaX software. The results showed that there are 67 different metabolites that can match the first order ion m / z of the database material and the second fragment ion of the database material，including mainly 19 amino acids and their derivatives,dipeptides,7 vitamins,4 carbohydrates and their derivatives, 11 nucleic acids and their derivatives, 4 alkaloids,4 flavors and 9 organic acids. 29 of which are up-regulated, 38 compounds are significantly decreased, indicating that SINAC4 transcription factors affected the metabolism of tomato fruit at the beginning of ripping.

Key words: tomato，transcription factor NAC4， metabolomics， metabolites