亚硒酸钠促进多形汉逊酵母DL-1合成谷胱甘肽的转录组学分析

摘要/Abstract

摘要： 摘要：为解析亚硒酸钠（Na2SeO3）诱导多形汉逊酵母DL-1(Hansenula polymorpha DL-1，HP-DL-1)生物合成谷胱甘肽(glutathione，GSH) 的分子机制。本研究通过DTNB法以及Illumina测序平台对比分析不同浓度Na2SeO3对HP-DL-1合成GSH产量的影响及Na2SeO3诱导下GSH高产菌株与出发菌株转录组差异。结果发现，以60 μmol/L Na2SeO3诱导HP-DL-1发酵48 h，酵母总GSH 产量达530.22 ± 9.6 mg /L；与对照组相比，Na2SeO3诱导组共有1254个显著性差异表达基因（differentially expressed genes，DEGs），其中630个DEGs表达上调，624个DEGs表达下调；依据GO（Gene Ontology）和KEGG（kyoto encyclopedia of genes and genomes）富集，这些差异基因主要集中在细胞周期、有丝分裂、氨基酸生物合成、糖酵解、核糖体组分、甲烷、脂肪、核酸、谷胱甘肽等多条代谢通路。本研究为进一步通过分子改造构建高产GSH的酵母工程菌株提供参考。

关键词: 多形汉逊酵母, 谷胱甘肽, 亚硒酸钠, 转录组, 差异表达基因

Abstract: Abstract:To illustrate the mechanism of glutathione biosynthesis by Hansenula polymorpha DL-1 exposed to sodium selenite (Na2SeO3), differentially expressed genes (DEGs) in the Na2SeO3-treated and untreated conditions were explored using a combination of transcriptomic sequencing and bioinformatic methods．The results showed that 60 μmol/L Na2SeO3 was found to favor the increase in GSH yield by the yeast cells, and the levels of GSH reached 530.22 ± 9.6 mg/L. A total of 1254 distinct differential expression genes (DEGs) was identified in the Na2SeO3 induced group, of which 630 genes were up-regulated, whereas the remaining 624 genes were down-regulated. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment of DEGs indicated that the genes involved in cell cycle, ribosome components and synthesis of biological molecules (amino acid, glycolysis, fat and nucleic acid) were up-regulated. In addition, genes encoding the synthase and kinase involved in the metabolism of glutathione and energy substances were up-regulated. This study provide adequate information for a better understanding of the physiological mechanism of GSH biosynthesis by H polymorpha, which also provided a theoretical support for subsequent molecular improvement of over-production GSH engineering strains.

Key words: Hansenula polymorpha, glutathione, sodium selenite, transcriptome, differential expression gene

中图分类号:

Q939.97

王婷刘婵婵任娟荆蓉蓉钱卫东. 亚硒酸钠促进多形汉逊酵母DL-1合成谷胱甘肽的转录组学分析[J]. 食品科学, 0, (): 0-0.

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