Evaluation of Cysteine Dioxygenase Activity in Marine Organisms by High Performance Liquid Chromatography

doi:10.7506/spkx1002-6630-201812027

Abstract

Abstract: An analytical method for cysteine sulfonate by high performance liquid chromatography (HPLC) was established and applied to determine the activity of cysteine dioxygenase in different marine organism tissues. Cysteine sulfinate was subjected to 3 min pre-column derivatization with o-phthalaldehyde (OPA) at a molar ratio of 1:2. The separation was performed on an Agilent Eclipse XDB-C18 using a mobile phase composed of methanol and sodium acetate (8:2, V/V) at a flow rate of 1 mL/min. The analyte was detected at 315 nm. The calibration curve showed a good linearity (R2 > 0.99) in the concentration range of 10–400 μg/mL. The relative standard deviations (RSDs) for parallel samples were 0.9%–6.4% and the retention time was 1.46 min. Using this method, cysteine dioxygenase activity was detected to be present in marine shellfish, crab, shrimp and fish, with the highest activity being observed in Penaeus vannamei. Among different tissues of fish, the highest activity of cysteine dioxygenase was detected in the liver, while the lowest was found in the gill.

Key words: seafood, cysteine dioxygenase, cysteine sulfinate, high performance liquid chromatography (HPLC)

CLC Number:

TS201.2

FAN Zirui, HU Jiafei, JIANG Ruini, YANG Xingling, JIN Huoxi. Evaluation of Cysteine Dioxygenase Activity in Marine Organisms by High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2018, 39(12): 174-178.

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Evaluation of Cysteine Dioxygenase Activity in Marine Organisms by High Performance Liquid Chromatography

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