Separation, Purification and Chemical Characterization of a Polysaccharide Fraction from Polygonatum cyrtonema Hua in Jiuhua Mountain

Abstract

Abstract: The conventional method, enzymolysis, ultrasonic extraction and ultrasonic-assisted enzymolysis were compared for their effectiveness in extracting polysaccharides from Polygonatum cyrtonema Hua in Jiuhua Mountain. Ultrasonic-assisted enzymolysis was evaluated as the best method due to the highest polysaccharide yield. The polysaccharide extract obtained by the selected method was a single polysaccharide component according to DEAE-cellulose column chromatographic analysis. Infrared spectral analysis revealed that the polysaccharide fraction was a hetero polysaccharide. Its relative molecular weight was determined by dextran gel filtration method to be 8912. Based on gas chromatographic analysis, its monosaccharide composition was composed of fructose and glucose at a ratio of 8.7:1.

Key words: polysaccharides from Polygonatum cyrtonema Hua, separation and purification, chemical characterization

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Separation, Purification and Chemical Characterization of a Polysaccharide Fraction from Polygonatum cyrtonema Hua in Jiuhua Mountain

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