Abstract: Objective: To explore the optimal extraction conditions of flavonoids from the fruits of Actinidia arguta. Methods: Flavonoids extraction was carried out using ethanol as the extraction solvent with the assistance of ultrasound. Response surface methodology based on Box-Behnken experimental design was used for process optimization. Results: The optimal extraction conditions were ethanol concentration of 70%, ultrasonic treatment time of 6 min and ultrasonic power of 270 W. Under these conditions, the experimental extraction rate of total flavonoids from Actinidia arguta fruits was 0.0297%, which was consistent with the prediction value. Conclusion: Ultrasound assistance is a suitable method for the extraction of total flavonoids from Actinidia arguta fruits.

Key words: Actinidia arguta, flavonoids, ultrasonic-assisted extraction, response surface methodology