FOOD SCIENCE ›› 0, Vol. ›› Issue (): 161-164.

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Comparison of Different Cell Wall Disruption Methods for Yeast Total RNA Extraction

  

  • Received:2010-09-06 Revised:2011-04-27 Online:2011-06-15 Published:2011-05-13

Abstract: Five methods such as liquid nitrogen grinding, repeated freeze-thawing, ultrasonic treatment, vortex shaking with glass beads and snailase hydrolysis were used to break the cell wall of yeast for the extraction of total RNA by the Trizol method. Based on total RNA concentration and purity, these methods were compared for their effects on total RNA extraction from yeast. The results showed liquid nitrogen grinding and repeated freeze-thawing were the most effective and convenient methods for total RNA extraction from yeast by Trizol.

Key words: Trizol, RNA extraction, yeast, cell wall disruption