Abstract: Ten kinds of commonly used macroporous resin were compared for their effectiveness in purifying crude red pigment obtained from Parthenocissus tricuspidate fruits by fractional solvent extraction. It was found that LSA-7 resin had the best purification performance. Further, operating conditions for purifying red pigment from Parthenocissus tricuspidate fruits by dynamic adsorption onto LSA-7 resin column were optimized and UV spectrophotometry and HPLC were used to analyze red pigment samples. The adsorption of LSA-7 resin almost reached its saturation under the following conditions: temperature of 30 ℃, sample concentration of 30 mg/mL and sample loading amount of 2 BV. Elution at a flow rate of 3 BV/h resulted in a red pigment purity of more than 96.7%. Moreover, the resulting pigment composition was the same as that obtained by thin layer silica gel H separation. Therefore, LSA-7 resin is suitable for industrial dynamic production of red pigment from Parthenocissus tricuspidate fruits.

Key words: Parthenocissus tricuspidate, red pigment, LSA-7 resin, dynamic adsorption